EDTA Scientific Study Data -- 100 Studies

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Table Of Contents

EDTA Scientific Study Data -- 100 Studies

Compiled by
Karl Loren

Results for your Medlines query:: Words in title only: EDTA; Published in 1977 through 1999; Only select references with abstracts available; Show references published in English only; Show references pertaining to humans

Documents: 1 to 100 of 309

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Number	Title	Comments
...1...	Sodium iron EDTA [NaFe(III)EDTA] as a food fortificant does not influence absorption and urinary excretion of manganese in healthy adults.
...2...	Sodium iron EDTA [NaFe(III)EDTA] as a food fortificant: the effect on the absorption and retention of zinc and calcium in women.
...3...	Platelet release reaction during EDTA-induced platelet agglutinations and inhibition of EDTA-induced platelet agglutination by anti-glycoprotein II b/III a complex monoclonal antibody.
...4...	A scanning electron microscopical study of normal and fluorotic enamel demineralized by EDTA.
...5...	A scanning electron microscopical study of normal and fluorotic enamel demineralized by EDTA.
...6...	Hydrogen cyanide poisoning: treatment with cobalt EDTA.
...7...	Effects of hyaluronidase, trypsin, and EDTA on surface composition and topography during detachment of cells in culture.
...8...	EDTA soluble protein of human mature normal enamel.
...9...	Complement components detected on normal red blood cells taken into EDTA and CPD.
...10...	A convenient method of DNA extraction from blood anticoagulated with EDTA.
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...11...	Fe(III)-EDTA complex as iron fortification. Further studies.
...12...	Morphological and cytochemical study of Chlamydia with EDTA regressive technique and Gautier staining in ultrathin frozen sections of infected cell cultures: a comparison with embedded material.
...13...	[51Cr]EDTA plasma clearance and endogenous creatinine clearance in advanced renal insufficiency.
...14...	Effects of Ca, Mg, and EDTA on creatine kinase activity in cerebrospinal fluid.
...15...	Compensation of dietary induced reduction of tetracycline absorption by simultaneous administration of EDTA.
...16...	Isolation and characterization of outer and inner membranes from Pseudomonas aeruginosa and effect of EDTA on the membranes.
...17...	Single injection (51Cr)EDTA plasma clearance determination in children using capillary blood samples.
...18...	Precision of single injection (51Cr)EDTA plasma clearance and endogenous creatinine clearance determinations in children.
...19...	IgG platelet antibodies in EDTA-dependent pseudothrombocytopenia bind to platelet membrane glycoprotein IIb.
...20...	Metrizamide in urography. II. A comparison of 51Cr-EDTA clearance and metrizamide clearance in man.
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...21...	Fe(III)-EDTA complex as iron fortification.
...22...	Restoration of hemoglobin function in stored EDTA blood. Application in identification of hemoglobin variants with abnormal oxygen affinity.
...23...	GFR measurement with iohexol and 51Cr-EDTA. A comparison of the two favoured GFR markers in Europe.
...24...	Tissue inhibitor of metalloproteinase-2 protection of matrix metalloproteinase-2 from degradation by plasmin is reversed by divalent cation chelator EDTA and the bisphosphonate alendronate.
...25...	Effects of smoking on the urine excretion of oral 51Cr EDTA in ulcerative colitis.
...26...	Ultrasonic subgingival root planing and EDTA etching in a one-step procedure.
...27...	Lead exposure and accumulation in healthy Thais: assessed by lead levels, EDTA mobilization and heme synthesis-related parameters.
...28...	Defining the orientation of the human U1A RBD1 on its UTR by tethered-EDTA(Fe) cleavage.
...29...	Essential monoclonal gammopathy with an IgM paraprotein that is a cryoglobulin with cold agglutinin and EDTA-dependent platelet antibody properties.
...30...	The role of EDTA in provoking allergic reactions to subcutaneous infusion of apomorphine in patients with Parkinson's disease: a histologic study.
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...31...	EDTA chelation therapy should be more commonly used in the treatment of vascular disease.	EDTA chelation therapy is safe, effective, and more economical than commonly used surgical treatments for vascular disease. This article includes evidence of effectiveness, mechanisms of action of EDTA, a discussion of studies that have been done regarding the therapy, and some brief case reports. The conclusion is that EDTA chelation therapy should be a therapeutic option for vascular disease, either by itself or in conjunction with standard protocols.
...32...	Fibrinogen present in EDTA--anticoagulated plasma stimulates the tissue-type plasminogen activator-catalysed conversion of plasminogen to plasmin.
...33...	Freeze-dried fibrinogen or fibrinogen in EDTA stimulate the tissue-type plasminogen activator-catalysed conversion of plasminogen to plasmin.
...34...	An EDTA-associated anti-B agglutinin: the role of ionized calcium.
...35...	Aminoglycosides prevent and dissociate the aggregation of platelets in patients with EDTA-dependent pseudothrombocytopenia.
...36...	Iron release from recombinant N-lobe and single point Asp63 mutants of human transferrin by EDTA.
...37...	Assessment of glomerular filtration rate utilizing subcutaneously injected 51Cr-EDTA.
...38...	The analysis of EDTA in dried bloodstains by electrospray LC-MS-MS and ion chromatography.
...39...	Human nasal absorption of 51Cr-EDTA in smokers and control subjects.
...40...	Screening for EDTA-dependent deviations in platelet counts and abnormalities in platelet distribution histograms in pseudothrombocytopenia.
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...41...	Comparison of manual and automated cell counts in EDTA preserved synovial fluids. Storage has little influence on the results.
...42...	Comparison of EDTA and acid-citrate-dextrose collection tubes for detection of cytomegalovirus antigenemia and infectivity in leukocytes before and after storage.
...43...	Synergistic effect of gramicidin and EDTA in inhibiting sperm motility and cervical mucus penetration in vitro.
...44...	End-stage renal failure due to analgesic nephropathy, its changing pattern and cardiovascular mortality. EDTA-ERA Registry Committee.
...45...	Comparison of heparin and EDTA transport tubes for detection of cytomegalovirus in leukocytes by shell vial assay, pp65 antigenemia assay, and PCR.
...46...	A new type of pseudothrombocytopenia: EDTA-mediated agglutination of platelets bearing Fab fragments of a chimaeric antibody.
...47...	EDTA-plasma vs serum differences in cholesterol, high-density-lipoprotein cholesterol, and triglyceride as measured by several methods.	We conclude that P/S ratios vary by analytical methods, and that HDLC ratios tend to be larger in magnitude and in the opposite direction from TC and TG. Both effects lead to significant biases in computed disease risk.
...48...	Random locomotion and chemotaxis of human blood polymorphonuclear leukocytes (PMN) in the presence of EDTA: PMN in close quarters require neither leukocyte integrins nor external divalent cations.
...49...	Long-term precision of glomerular filtration rate measurements using 51Cr-EDTA plasma clearance.
...50...	Technetium (99mTc)-labelled white cell scanning, 51Cr-EDTA and 14C-mannitol-labelled intestinal permeability studies: non-invasive methods of diagnosing acute intestinal graft-versus-host disease [see comments]
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...51...	EDTA dependent pseudothrombocytopenia caused by antibodies against the cytoadhesive receptor of platelet gpIIB-IIIA.
...52...	Dynamic positron emission tomography for study of cerebral hemodynamics in a cross section of the head using positron-emitting 68Ga-EDTA and 77Kr.
...53...	Sequential preparation of highly purified microvillous and basal syncytiotrophoblast membranes in substantial yield from a single term human placenta: inhibition of microvillous alkaline phosphatase activity by EDTA.
...54...	Simultaneous urography and determination of glomerular filtration rate. A comparison of total plasma clearances of iohexol and 51Cr-EDTA in plegic patients.
...55...	Oxidation of human insulin-like growth factor I in formulation studies. 3. Factorial experiments of the effects of ferric ions, EDTA, and visible light on methionine oxidation and covalent aggregation in aqueous solution.
...56...	In vivo inhibition of serum dopamine-beta-hydroxylase by CaNa2 EDTA injection.
...57...	Effect of different concentrations of EDTA on smear removal and collagen exposure in periodontitis-affected root surfaces.
...58...	Inhibition of paraoxonase activity in human liver microsomes by exposure to EDTA, metals and mercurials.
...59...	90Y-labeled antibody uptake by human tumor xenografts and the effect of systemic administration of EDTA.
...60...	Quantitative capillary electrophoresis/ion spray tandem mass spectrometry determination of EDTA in human plasma and urine.
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...61...	EDTA and the absorption of iron from food.
...62...	Back pain following epidural anesthesia with 2-chloroprocaine (EDTA-free) or lidocaine [see comments]
...63...	Clinical evaluation of the efficacy of EDTA solution as an endodontic irrigant.	These results suggest that 15% EDTA solution is more effective than saline solution as a root canal irrigant.
...64...	Renal and extrarenal clearance of 99mTc-MAG3: a comparison with 125I-OIH and 51Cr-EDTA in patients representing all levels of glomerular filtration rate.
...65...	Analysis of 15 cases with platelet EDTA-dependent antibodies.
...66...	A novel method for isolation of Chlamydia pneumoniae by treatment with trypsin or EDTA.
...67...	Reproducibility of simplified techniques for the measurement of 51Cr-EDTA clearance.
...68...	How good is the slope of the second exponential for estimating 51Cr-EDTA renal clearance?
...69...	The cytotoxic interaction of inorganic trace elements with EDTA and cisplatin in sensitive and resistant human ovarian cancer cells.
...70...	Removing IgG antibodies from intact red cells: comparison of acid and EDTA, heat, and chloroquine elution methods.
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...71...	Flow cytometric evaluation of platelet activation in blood collected into EDTA vs. Diatube-H, a sodium citrate solution supplemented with theophylline, adenosine, and dipyridamole [see comments]
...72...	Report on management of renale failure in Europe, XXVI, 1995. Rare diseases in renal replacement therapy in the ERA-EDTA Registry.
...73...	Metal excretion and magnesium retention in patients with intermittent claudication treated with intravenous disodium EDTA.	Sixty patients with intermittent claudication participated in a double-blind placebo-controlled trial of 20 courses of intravenous chelation therapy with 3 g of disodium EDTA vs placebo during 5-9 weeks. After the first infusion, the 24-h urinary excretion of lead and zinc was approximately 25-fold higher in the EDTA-treated group; relative differences for copper and calcium were smaller. Urinary magnesium excretion in the EDTA-treated group was one-third less than in the control group. After the treatment period, the blood lead concentration had decreased by approximately 73% and the serum zinc concentration by approximately 34%; other changes in blood concentrations were negligible. The loss of essential minerals and the possible redistribution of lead in the body may constitute a disadvantage that should be taken into account in repeated intravenous EDTA treatment.
...74...	Smear removal and collagen exposure after non-surgical root planing followed by etching with an EDTA gel preparation.
...75...	EDTA-dependent pseudothrombocytopenia: a clinical and epidemiological study of 112 cases, with 10-year follow-up.
...76...	Measurement of glomerular filtration rate by single-injection, single-sample techniques, using 51Cr-EDTA or iohexol.
...77...	More on false thrombocytopenias: EDTA-dependent pseudothrombocytopenia associated with a congenital platelet release defect.
...78...	C-reactive protein: the difference between quantitation is serum and EDTA plasma.
...79...	Production and characterization of monoclonal antibodies to the EDTA extract of Leptospira interrogans, serovar icterohaemorrhagiae.
...80...	EDTA chelation therapy in the treatment of vascular disease [see comments]	Ethylenediamine tetraacetic acid (EDTA) chelation therapy has been used for decades for the treatment of vascular disease, alone or in combination with other treatments. This article includes a historic review of the research literature, current evidence of effectiveness, potential mechanisms of action of EDTA, and some brief case reports. The authors conclude that EDTA chelation therapy is a valuable therapeutic option for vascular disease, either alone or in conjunction with standard treatment protocols.
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...81...	Plasma clearance rate of 51Cr-EDTA provides a precise and convenient technique for measurement of glomerular filtration rate in diabetic humans.
...82...	Survival modelling in kidney transplantation: hazard rates of graft loss. Transplant Working Group of the Registry Committee of the European Dialysis and Transplantation Association-European Renal Association (EDTA-ERA).
...83...	Malignancies after renal transplantation: the EDTA-ERA registry experience. European Dialysis and Transplantation Association-European Renal Association.
...84...	Transplantation Report. 2: Pre-emptive renal transplantation in adults aged over 15 years. The EDTA-ERA Registry. European Dialysis and Transplant Association-European Renal Association.
...85...	Absorption-enhancing mechanism of EDTA, caprate, and decanoylcarnitine in Caco-2 cells.
...86...	Repeated successful pregnancies after kidney transplantation in 102 women (Report by the EDTA Registry).
...87...	LPS induced release of IL-1 beta, IL-6, IL-8 and TNF-alpha in EDTA or heparin anticoagulated whole blood from persons with high or low levels of serum HDL.
...88...	Incidence and diagnosis of EDTA-dependent pseudothrombocytopenia in a consecutive outpatient population referred for isolated thrombocytopenia.
...89...	Provocative chelation with DMSA and EDTA: evidence for differential access to lead storage sites.	Both intravenously administered EDTA and an orally administered alternative proved to be effective in removing lead from the body.
...90...	EDTA-dependent pseudothrombocytopenia. Association with antiplatelet and antiphospholipid antibodies.
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...91...	Transverse relaxation of saline and plasma using Mn(II), HSA-EDTA-Mn, and HSA-EDTA-Gd: application to erythrocyte water exchange.
...92...	Toxicological profile, current use, and regulatory issues on EDTA compounds for assessing use of sodium iron EDTA for food fortification.
...93...	Agglutination of an EDTA blood sample caused by an EDTA-dependent panagglutinin.
...94...	Combined treatment of medullary sponge kidney by EDTA potassium citrate and extracorporeal shock wave lithotripsy.
...95...	Evaluation of the EDTA-washed diet for use in the experimental production of zinc deficiency in human subjects.	Ethylenediaminetetraacetate (EDTA) is known to bind zinc (Zn) and other metals. [It will bind these metals IN THE GUT as well as in the blood. Thus, when the EDTA is taken orally, it WILL bind metals, thus its use here. There is no inference that this EDTA was to bind metals in the blood.] EDTA-washed soy protein-based diet has been extensively used as a dietary model for the production of Zn-deficiency in human subjects as well as in experimental animals.
...96...	Injurious effect of EDTA contamination on colorimetry of serum iron.
...97...	EDTA clearance in monitoring cisplatin dose escalation in patients with bulky metastatic germ cell tumors of the testis.
...98...	Studies on EDTA extracts and collagenase digests from osteoporotic cancellous bone of the femoral head.
...99...	Determination of cyclic 3'-5'-adenosine monophosphate in plasma by RIA methods in the presence of EDTA.
...100..	The blood tumour barrier in intracranial tumours studied with X-ray computed tomography and positron emission tomography using 68-Ga-EDTA.

HealthGate Documents

Record 1 from database: MEDLINE
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Title: Sodium iron EDTA [NaFe(III)EDTA] as a food fortificant does not influence absorption and urinary excretion of manganese in healthy adults.
Author: Davidsson L; Almgren A; Hurrell RF
Address: Laboratory for Human Nutrition, Swiss Federal Institute of Technology, ETH ZÂurich, CH-8803 RÂuschlikon, Switzerland.
Source: J Nutr, 1998 Jul, 128:7, 1139-43
Abstract: NaFe(III)EDTA is a promising iron (Fe) compound for food fortification programs because of its high Fe bioavailability from meals containing dietary inhibitors of Fe absorption such as phytic acid. However, this Fe compound is not currently used in any large-scale fortification program because of concern over its possible negative influence on the metabolism of other essential minerals or its possible influence on the absorption of potentially toxic elements, such as manganese (Mn). In this study, Mn absorption and urinary excretion were studied in adults after intake of an Fe-fortified weaning cereal labeled with 54Mn. In a crossover design, the fortification of the weaning cereal with Fe as NaFeEDTA was compared with ferrous sulfate. Manganese absorption was measured by extrapolation from whole-body retention data 10-30 d after intake, and urinary excretion of 54Mn was measured over 7 d. No significant differences in 54Mn absorption or urinary excretion were found; 1.1 +/- 0.15 and 0.91 +/- 0.35% of the ingested dose was absorbed from the cereal fortified with NaFe(III)EDTA and FeSO4, respectively. Urinary excretion of 54Mn was very low; the total radioactivity in urine represented 1.1 +/- 0.55% of the absorbed dose with NaFe(III)EDTA and 0.72 +/- 0.53% of the absorbed dose with FeSO4. Until now, Fe-fortification programs have met with only limited success. The introduction of NaFeEDTA as a food fortificant could be a useful tool to provide bioavailable Fe to vulnerable groups in the population and thus aid in combating Fe deficiency.
Language of Publication: English
Unique Identifier: 98315238

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MeSH Heading (Major): Ferric Compounds|AD/*AE; Food, Fortified|*; Iron Chelating Agents|*; Manganese|AN/*PK/UR
MeSH Heading: Absorption; Adult; Cereals|CH; Cross-Over Studies; Edetic Acid|AD/AE; Female; Ferrous Compounds|AD; Human; Infant Food|AN; Iron|AN; Male; Middle Age; Nutritional Status; Phytic Acid|AN; Radioisotopes; Weaning

Publication Type: JOURNAL ARTICLE
ISSN: 0022-3166
Country of Publication: UNITED STATES

Record 2 from database: MEDLINE
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Title: Sodium iron EDTA [NaFe(III)EDTA] as a food fortificant: the effect on the absorption and retention of zinc and calcium in women.
Author: Davidsson L; Kastenmayer P; Hurrell RF
Address: NestlÆe Research Centre, Nestec Ltd, Lausanne, Switzerland.
Source: Am J Clin Nutr, 1994 Aug, 60:2, 231-7
Abstract: The iron fortificant NaFeEDTA could have a potential negative effect on the metabolism of other minerals. We have used stable isotopes to monitor zinc and calcium metabolism in 10 women consuming a single meal of high-extraction wheat rolls (100 g flour) fortified with 5 mg Fe as either FeSO4 or NaFeEDTA. Six-day chemical balances were made simultaneously to study apparent zinc and calcium retention from the complete diet containing the differently iron-fortified breads (200 g flour; 10 mg added Fe/d). Mean 70Zn absorption from the bread meal increased from 20.9% with FeSO4 to 33.5% with NaFeEDTA (P < 0.05) whereas mean 44Ca absorption was 53.3% from both breads. When NaFeEDTA-fortified bread was consumed, there was a small but significant increase in urinary excretion of 70Zn and 44Ca. There was a similar small increase in urinary zinc excretion during the 6-d balance, although the apparent retention of zinc and calcium was not different. Thus, we found no negative overall effect of NaFeEDTA consumption on the metabolism of zinc and calcium. In contrast, the results suggest that NaFeEDTA added to low-bioavailability diets might increase zinc absorption as well as provide iron with high bioavailability.
Language of Publication: English
Unique Identifier: 94303631

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MeSH Heading (Major): Calcium, Dietary|AD/*PK; Edetic Acid|*AD; Ferric Compounds|*AD; Food, Fortified|*; Iron Chelating Agents|*AD; Zinc|AD/*PK
MeSH Heading: Absorption; Adult; Biological Availability; Bread; Comparative Study; Diet; Female; Ferrous Compounds|AD; Human; Iron|BL; Middle Age

Publication Type: CLINICAL TRIAL; JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
ISSN: 0002-9165
Country of Publication: UNITED STATES

Record 3 from database: MEDLINE
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Title: Platelet release reaction during EDTA-induced platelet agglutinations and inhibition of EDTA-induced platelet agglutination by anti-glycoprotein II b/III a complex monoclonal antibody.
Author: Ryo R; Sugano W; Goto M; Takada M; Saigo K; Hashimoto M; Yamaguchi N
Address: Blood Transfusion Service, Kobe University Hospital, Japan.
Source: Thromb Res, 1994 May, 74:3, 265-72
Abstract: To characterize the nature of EDTA-induced platelet agglutination, the spontaneous release of beta-thromboglobulin (beta-TG) and platelet factor 4 (PF4) was examined during EDTA-induced platelet agglutinations. A slight release of beta-TG and PF4 was observed when EDTA-anticoagulated whole blood from cases with EDTA-induced platelet agglutination was kept for 60 minutes, whereas a high spontaneous release of these proteins was found from normal blood anticoagulated with EDTA. These findings imply that EDTA-dependent platelet agglutinin may stabilize the platelet membrane surfaces. Secondly, we found that pretreatment of fresh blood with anti-glycoprotein (GP) II b/III a complex monoclonal antibody dramatically reduced EDTA-induced platelet agglutinations. This study indicated that the binding sites of EDTA-dependent antibody might be GP II b/III a complex. The use of an anti-GP II b/III a complex monoclonal antibody may be useful in avoiding analytical errors in some cases with EDTA-induced pseudothrombocytopenia.
Language of Publication: English
Unique Identifier: 94317160

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MeSH Heading (Major): beta-Thromboglobulin|*ME; Blood Platelets|*ME; Platelet Factor 4|*ME; Platelet Membrane Glycoproteins|*IM
MeSH Heading: Adenosine Diphosphate; Antibodies, Monoclonal; Edetic Acid; Hemagglutination|DE; Human

Publication Type: JOURNAL ARTICLE
ISSN: 0049-3848
Country of Publication: UNITED STATES

Record 4 from database: MEDLINE
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Title: A scanning electron microscopical study of normal and fluorotic enamel demineralized by EDTA.
Author: Thylstrup A
Address
Source: Acta Odontol Scand, 1979, 37:3, 127-35
Abstract: Normal and fluorotic primary and permanent teeth were demineralized in 10% EDTA at pH 7 for varying periods of time up to 4 weeks. The fluorotic teeth initially dissolved at a much slower rate than non-fluorotic specimens and appeared to contain more organic material. After almost complete removal of the outer enamel a soft organic layer remained on the dentin surface. This consisted of a fine fibrillar mesh and some more dense material. After four weeks much of the organic material had disappeared leaving only a thin membrane on the dentin surface.
Language of Publication: English
Unique Identifier: 80016719

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MeSH Heading (Major): Dental Enamel|ME/*PA/UL; Dental Enamel Proteins|*ME; Edetic Acid|*PD; Fluorosis, Dental|ME/*PA
MeSH Heading: Human; Microscopy, Electron, Scanning; Minerals|ME

Publication Type: JOURNAL ARTICLE
ISSN: 0001-6357
Country of Publication: FINLAND

Record 5 from database: MEDLINE
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Title: A scanning electron microscopical study of normal and fluorotic enamel demineralized by EDTA.
Author: Thylstrup A
Address
Source: Acta Odontol Scand, 1979, 37:2, 127-35
Abstract: Normal and fluorotic primary and permanent teeth were demineralized in 10% EDTA at pH 7 for varying periods of time up to 4 weeks. The fluorotic teeth initially dissolved at a much slower rate than non-fluorotic specimens and appeared to contain more organic material. After almost complete removal of the outer enamel a soft organic layer remained on the dentin surface. This consisted of a fine fibrillar mesh and some more dense material. After four weeks much of the organic material had disappeared leaving only a thin membrane on the dentin surface.
Language of Publication: English
Unique Identifier: 79184364

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MeSH Heading (Major): Dental Enamel|DE/*UL; Dental Enamel Proteins|*ME; Edetic Acid|*PD; Fluorosis, Dental|ME/*PA
MeSH Heading: Human; Microscopy, Electron, Scanning; Minerals|ME

Publication Type: JOURNAL ARTICLE
ISSN: 0001-6357
Country of Publication: FINLAND

Record 6 from database: MEDLINE
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Title: Hydrogen cyanide poisoning: treatment with cobalt EDTA.
Author: Nagler J; Provoost RA; Parizel G
Address
Source: J Occup Med, 1978 Jun, 20:6, 414-6
Abstract: Three case reports are presented of employees who suffered varying degrees of exposure to hydrogen cyanide and their subsequent clinical courses following treatment with cobalt EDTA. A review of treatment modalities for CN- toxicity is given. It is concluded that, because of the degree of patient symptomatology associated from the use of cobalt EDTA, this therapy be reserved only for patients with the most severe degress of exposure to CN(-), and that in all other cases combined sodium nitrite and sodium thiosulphate therapy should be employed.
Language of Publication: English
Unique Identifier: 78219996

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MeSH Heading (Major): Cobalt|*TU; Edetic Acid|AE/*TU; Hydrogen Cyanide|*PO; Occupational Diseases|*CI/DT
MeSH Heading: Accidents, Occupational; Adult; Case Report; Environmental Exposure; Human; Male; Middle Age; Nitrites|TU; Sodium|TU; Thiosulfates|TU

Publication Type: JOURNAL ARTICLE
ISSN: 0096-1736
Country of Publication: UNITED STATES

Record 7 from database: MEDLINE
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Title: Effects of hyaluronidase, trypsin, and EDTA on surface composition and topography during detachment of cells in culture.
Author: Vogel KG
Address: Department of Anatomy, University of New Mexico, School of Medicine, Albuquerque 87131.
Source: Exp Cell Res, 1978 May, 113:2, 345-57
Abstract: Cultured human embryo fibroblasts (HLM18) were labeled with [3H]glucosamine and Na35SO4, and then treated with testicular hyaluronidase, trypsin, or EDTA. Macromolecular material from the surface of these cells was characterized by DEAE-cellulose chromatography and cetylpyridinium chloride precipitation while the associated morphology of cell detachment was studied by phase contrast and scanning electron microscopy. Release of surface glycosaminoglycans by testicular hyaluronidase did not cause cell rounding or detachment. EDTA did not release cell-surface components, but caused cell contraction and detachment morphologically similar to that caused by trypsin. Large amounts of cell-surface glycoproteins and glycosaminoglycans were released by trypsin. From these observations it is concluded that hyaluronic acid is not a principal adhesive agent in the attachment of cells to a substrate. It is suggested that both EDTA and trypsin may have their primary effect upon the cytoskeleton.
Language of Publication: English
Unique Identifier: 88329276

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MeSH Heading (Major): Cell Membrane|*DE/PH/UL; Edetic Acid|*PD; Hyaluronidase|*PD; Trypsin|*PD
MeSH Heading: Cell Adhesion|DE; Cell Line; Cytoskeleton|DE/PH; Glycosaminoglycans|PH; Human; Support, U.S. Gov't, P.H.S.

Publication Type: JOURNAL ARTICLE
ISSN: 0014-4827
Country of Publication: UNITED STATES
CAS Registry/EC Number: EC 3.2.1.35 (Hyaluronidase); EC 3.4.21.4 (Trypsin); 0 (Glycosaminoglycans); 60-00-4 (Edetic Acid)

Record 8 from database: MEDLINE
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Title: EDTA soluble protein of human mature normal enamel.
Author: Belcourt A; Gillmeth S
Address
Source: Calcif Tissue Int, 1979 Nov 6, 28:3, 227-31
Abstract: Pure human mature enamel was prepared using a careful microdissection technique. After EDTA dissolution, the soluble proteins were recovered representing a concentration of 0.035% in the initial enamel. When the samples were analyzed with polyacrylamide gel electrophoresis, Coomassie Brilliant Blue staining revealed only one sharp fast migrating band, whereas o-toluidine blue, methylene blue, Amido Black 10B, and pyronine red G showed a thin double band at the same migration distance. Ultracentrifugation studies suggested that the proteins were of low molecular weight or of weak density. Absorption spectra showed a strong absorbance at 260 nm. After hydrolysis, amino acid analyses yielded a composition of 25% Gly, 13.5% Glu, 11% Ser, and 11% Pro. Cysteine measured as cysteic acid was present at 2%, and 2% hydroxyproline was found. A carbohydrate content of 15% was estimated by the anthrone method. Glucose, galactose, mannose, and fucose, identified through gas chromatography, were in a molar ratio of 9:4:3:1. Thus the organic matrix of adult human enamel consists of one or possibly two acidic glycoproteins.
Language of Publication: English
Unique Identifier: 80066760

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MeSH Heading (Major): Dental Enamel Proteins|*AN
MeSH Heading: Amino Acids|AN; Carbohydrates|AN; Edetic Acid; Electrophoresis, Polyacrylamide Gel; Glycoproteins|AN; Human; Molecular Weight; Solubility

Publication Type: JOURNAL ARTICLE
ISSN: 0171-967X
Country of Publication: UNITED STATES

Record 9 from database: MEDLINE
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Title: Complement components detected on normal red blood cells taken into EDTA and CPD.
Author: Freedman J; Massey A
Address
Source: Vox Sang, 1979, 37:1, 1-8
Abstract: Normal red blood cells (RBC) from fresh EDTA and CPD blood and from stored CPD blood were examined for the presence of bound subcomponents of C3 and C4. By serologic agglutination tests, only C3d was detectable on the cells. Incubation in compatible fresh normal serum (FNS) at 37 degrees C appeared to increase the amount of 3Cd on the RBC. C3b was serologically detectable only on stored CPD cells and only after incubation in compatible FNS. No. C4 components were detected on the cell surfaces in agglutination tests. Using an indirect labeling technique, small, but significant, amounts of C3d and C4d were found on all three types of untreated cells. C3b was present on stored CPD cells only. The indirect labeling technique showed a significant increase in C3d and C4d on all cells following incubation i- compatible FNS, whereas bound C3b was significantly increased only with stored CPD cells. There was no increase in bound C4b following serum incubation. The average number of C3d molecules per cell on normal EDTA cells was 557 and average Ko was 3.6 x 10(7) l/mol.
Language of Publication: English
Unique Identifier: 80037735

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MeSH Heading (Major): Citrates|*PD; Complement 3|*IM; Complement 4|*IM; Edetic Acid|*PD; Erythrocytes|*IM
MeSH Heading: Animal; Binding, Competitive; Coombs' Test; Haplorhini; Hemagglutination Tests; Human; Immune Sera|PD; Iodine Radioisotopes; Rabbits

Publication Type: JOURNAL ARTICLE
ISSN: 0042-9007
Country of Publication: SWITZERLAND

Record 10 from database: MEDLINE
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Title: A convenient method of DNA extraction from blood anticoagulated with EDTA.
Author: Yokota M; Sindo K; Hiyoshi M; Tsuda I; Tatsumi N
Address: Department of Clinical and Laboratory Medicine, Osaka City University Medical School, Japan.
Source: Biochem Mol Biol Int, 1998 Jul, 45:3, 617-22
Abstract: Surplus blood often remains after routine clinical tests using EDTA-anticoagulated samples. To use this blood for DNA analysis, we isolated white cells by adherence to polyethylene terephthalate fibers, which could be stored for several weeks transported by mail after methanol fixation. DNA yield was sufficient and correlated with white cell count. Extracted DNA was free of hemoglobin contamination and durable to polymerase chain reaction and enzyme digestion, which yielded products visualized as well-separated bands on electrophoresis. We found our method to be practical for the routine clinical laboratory.
Language of Publication: English
Unique Identifier: 98344606

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MeSH Heading (Major): Cell Separation|*MT; DNA|*BL/IP; Leukocytes|*
MeSH Heading: Adult; Anticoagulants|PD; Edetic Acid|PD; Filtration|MT; Gene Amplification; Genotype; Human; HLA-DQ Antigens|CL/GE; Methanol; Polyethylene Terephthalates; Polymerase Chain Reaction

Publication Type: JOURNAL ARTICLE
ISSN: 1039-9712
Country of Publication: AUSTRALIA
CAS Registry/EC Number: 0 (Anticoagulants); 0 (HLA-DQ Antigens); 0 (HLA-DQA1); 0 (Polyethylene Terephthalates); 60-00-4 (Edetic Acid); 67-56-1 (Methanol); 9007-49-2 (DNA)

Record 11 from database: MEDLINE
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Title: Fe(III)-EDTA complex as iron fortification. Further studies.
Author: Martínez-Torres C; Romano EL; Renzi M; Layrisse M
Address
Source: Am J Clin Nutr, 1979 Apr, 32:4, 809-16
Abstract: The data presented confirm the advantages of Fe(III)-EDTA as a salt for iron fortification. This iron compound exchanges completely with intrinsic wheat iron in the lumen of the gut. The iron absorption data from this salt tested with six different food vehicles compared with the absorption of ferrous sulfate administered with the same vehicles indicate that while the mean absorption from ferrous sulfate varies from 2 to 30% according to the food vehicle mixed with the salt, the absorption from Fe(III)-EDTA remains practically the same. Apparently, the iron absorption from Fe(III)-EDTA complex is slightly or not affected by the presence of vegetable foods or milk. All these data suggest that only a small amount of iron from this salt, about 10 mg/day, would be necessary to prevent iron deficiency anemia even in those populations relying for their subsistence on vegetable food only.
Language of Publication: English
Unique Identifier: 79162501

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MeSH Heading (Major): Edetic Acid|*; Food, Fortified|*; Iron Chelates|*ME
MeSH Heading: Absorption; Anemia, Hypochromic|PC; Comparative Study; Dietary Carbohydrates; Female; Ferric Compounds|ME; Ferritin|BL; Ferrous Compounds|ME; Hemoglobins|ME; Human; Male; Structure-Activity Relationship; Sulfates; Transferrin|ME; Wheat

Publication Type: JOURNAL ARTICLE
ISSN: 0002-9165
Country of Publication: UNITED STATES

Record 12 from database: MEDLINE
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Title: Morphological and cytochemical study of Chlamydia with EDTA regressive technique and Gautier staining in ultrathin frozen sections of infected cell cultures: a comparison with embedded material.
Author: Popov V; Eb F; Lefebvre JF; Orfila J; Viron A
Address
Source: Ann Microbiol (Paris), 1978 Oct, 129 B:3, 313-37
Abstract: The cryo-ultramicrotomy technique was applied to study the ultrastructure of Chlamydia using two strains: one of C. psittaci and one of C. trachomatis. It clearly appeared that in both strains reticulate bodies show a high degree of plasticity, contrasting with the rigid spherical appearance of elementary bodies. Ultrastructural cytochemical study shows DNA fibrils dispersed throughout the cytoplasm in reticulate bodies whereas DNA is condensed in a nucleoid in elementary and intermediate bodies. The EDTA regressive technique reveals ribonucleoproteins in reticulate and elementary bodies of both studied strains.
Language of Publication: English
Unique Identifier: 79143304

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MeSH Heading (Major): Chlamydia psittaci|*UL; Chlamydia trachomatis|*UL; Edetic Acid|*; Frozen Sections|*; Microtomy|*
MeSH Heading: Animal; Bacteriological Techniques; Cattle; Cell Wall|AN/UL; Chick Embryo; Comparative Study; Culture Media; DNA, Bacterial|IP; Female; Histocytochemistry; Human; Microscopy, Electron; Ribonucleoproteins|IP

Publication Type: JOURNAL ARTICLE
ISSN: 0300-5410
Country of Publication: FRANCE

Record 13 from database: MEDLINE
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Title: [51Cr]EDTA plasma clearance and endogenous creatinine clearance in advanced renal insufficiency.
Author: Svendsen UG; Munck O; Czartoryski A; Stafanger G
Address
Source: Scand J Clin Lab Invest, 1978 Dec, 38:8, 777-9
Abstract: Comparison of [51Cr]EDTA plasma clearance corrected for extrarenal elimination with 24 h endogenous creatinine clearance in patients with advanced renal failure showed that the corrected [51Cr]EDTA clearance was lower than creatinine clearance, and thus might be a better approximation to the glomerular filtration rate in uraemic patients. The corrections cannot be used on [51cr]EDTA clearance values below the mean extrarenal clearance, averaging 3.7 ml/min.
Language of Publication: English
Unique Identifier: 79117847

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MeSH Heading (Major): Creatinine|*ME; Edetic Acid|*BL; Kidney Failure, Chronic|*ME
MeSH Heading: Adult; Aged; Chromium Radioisotopes; Comparative Study; Female; Human; Male; Middle Age

Publication Type: JOURNAL ARTICLE
ISSN: 0036-5513
Country of Publication: NORWAY

Record 14 from database: MEDLINE
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Title: Effects of Ca, Mg, and EDTA on creatine kinase activity in cerebrospinal fluid.
Author: Urdal P; Str‡mme JH
Address
Source: Clin Chem, 1979 Jan, 25:1, 147-50
Abstract: For one to obtain a precise estimate of creatine kinase (CK) activity in cerebrospinal fluid, the sample fraction is increased by about 10-fold over that used for serum. This increases the concentration of interfering substances, Ca being especially important. Therefore, the relationship between Ca, Mg, and EDTA was examined. Enzyme activity was maximal with 15 mmol of Mg per liter in the presence of 3 mmol of EDTA per liter, otherwise according to the (Scandinavian) recommended conditions for determination of CK activity in serum. These modifications increased the activity of CK by 35% for CK-MM and by 60% for CK-BB. Counteraction of Ca-induced inhibition was the main reason to this increase. We describe a practical and sensitive method for determining CK in cerebrospinal fluid.
Language of Publication: English
Unique Identifier: 79105833

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MeSH Heading (Major): Calcium|*PD; Creatine Kinase|*CF; Edetic Acid|*PD; Magnesium|*PD
MeSH Heading: Buffers; Enzyme Activation; Human; Kinetics

Publication Type: JOURNAL ARTICLE
ISSN: 0009-9147
Country of Publication: UNITED STATES

Record 15 from database: MEDLINE
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Title: Compensation of dietary induced reduction of tetracycline absorption by simultaneous administration of EDTA.
Author: Poiger H; Schlatter C
Address
Source: Eur J Clin Pharmacol, 1978 Nov 16, 14:2, 129-31
Abstract: The absorption of tetracycline in man under the influence of concomitantly administered EDTA, milk and a combination of EDTA and milk has been investigated. Urinary excretion of the drug was measured for 30 h. The inhibitory effect of milk could be counteracted by simultaneous ingestion of EDTA, which resulted in almost equivalent urinary excretion of tetracycline compared to experiments done in the fasting state. Administration of EDTA alone, in a neutral dosage form, did not significantly change absorption of the drug, which contradicted previous findings. The possible use of EDTA during tetracycline therapy is discussed.
Language of Publication: English
Unique Identifier: 79065107

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MeSH Heading (Major): Edetic Acid|*PD; Food|*; Intestinal Absorption|*DE; Tetracycline|*ME/UR
MeSH Heading: Adult; Female; Human; Male; Time Factors

Publication Type: JOURNAL ARTICLE
ISSN: 0031-6970
Country of Publication: GERMANY, WEST

Record 16 from database: MEDLINE
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Title: Isolation and characterization of outer and inner membranes from Pseudomonas aeruginosa and effect of EDTA on the membranes.
Author: Matsushita K; Adachi O; Shinagawa E; Ameyama M
Address
Source: J Biochem (Tokyo), 1978 Jan, 83:1, 171-81
Abstract: The outer and inner cytoplasmic membranes of Pseudomonas aeruginosa were separated as small and large membranes, respectively, from the cell envelope of this organism treated with lysozyme in Tris-chloride buffer containing sucrose and MgCl2 by differential centrifugation. The small membrane fraction contained predominantly 2-keto-3-deoxyoctonate (KDO), and little cytochromes or oxidase activities. The small membrane was composed of only 9 polypeptides and showed homogeneous small vesicles electron-microscopically. On the other hand, the large membrane fraction had high cytochrome contents and oxidase activities, and little KDO. The large membrane was composed of a number of polypeptides and showed large fragments or vesicles electron-microscopically. These results indicate that the small and large membranes are the outer and inner cytoplasmic membranes of P. aeruginosa, respectively. The isolated outer membrane showed a symmetrical protein peak with a density of 1.23 on sucrose density gradient centrifugation and the isolated inner membrane showed an unusually high density, probably due to association with ribosomes and extrinsic or loosely bound proteins. EDTA lowered the density of both membranes and caused lethal damage to the outer membrane, causing disintegration with the release of lipopolysaccharide (LPS), proteins and phospholipid.
Language of Publication: English
Unique Identifier: 78109407

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MeSH Heading (Major): Cell Membrane|*/AN/UL; Edetic Acid|*PD; Pseudomonas aeruginosa|*
MeSH Heading: Carbohydrate Dehydrogenases|AN; Cytochromes|AN; Female; Gluconates; Human; Ketoses|AN; Membrane Proteins|AN; NADH, NADPH Oxidoreductases|AN; Phospholipids|AN; Sugar Acids|AN

Publication Type: JOURNAL ARTICLE
ISSN: 0021-924X
Country of Publication: JAPAN

Record 17 from database: MEDLINE
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Title: Single injection (51Cr)EDTA plasma clearance determination in children using capillary blood samples.
Author: Brochner-Mortensen J; Christoffersen J
Address
Source: Scand J Clin Lab Invest, 1977 Nov, 37:7, 631-3
Abstract: The reliability of a determination of the total [51Cr]EDTA plasma clearance (E) (and with it the glomerular filtration rate), by a simplified single injection method (injected dose: 4.5 muCi per kg b.w.) using capillary blood samples (0.2 ml), was investigated in twenty children. Clearance values determined from capillary blood samples did not differ significantly from those measured simultaneously from venous blood samples, the mean ratio +/-SD being 1.02 +/- 0.06 (n = 10). The reproducibility (total day-to-day variation) of E determined from capillary blood samples was 6.7% in children with decreased renal function (n = 3) and 6.9% in children with normal renal function (n = 7). The present data indicate that the use of capillary blood samples is an accurate and very precise approach for determination of E in children.
Language of Publication: English
Unique Identifier: 78074717

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MeSH Heading (Major): Edetic Acid|*DU; Glomerular Filtration Rate|*
MeSH Heading: Child, Preschool; Chromium Radioisotopes|DU; Human; Infant; Methods

Publication Type: JOURNAL ARTICLE
ISSN: 0036-5513
Country of Publication: NORWAY

Record 18 from database: MEDLINE
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Title: Precision of single injection (51Cr)EDTA plasma clearance and endogenous creatinine clearance determinations in children.
Author: Brochner-Mortensen J; Rohbrandt K; Lauritzen RB
Address
Source: Scand J Clin Lab Invest, 1977 Nov, 37:7, 625-9
Abstract: The precision of two different clearance methods as used for routine assessment of the glomerular filtration rate (GFR) was investigated in thirty-one children aged 0.6-14 years: total [51Cr]EDTA plasma clearance (E) determined by a simplified single injection method; and 24 h endogenous creatinine clearance (C). Determination of C twice only succeeded in twenty children because of problems in collecting 24 h urine accurately. The precision (determined from the total day-to-day variation) for single determinations in patients with E greater than or equal to 80 ml/min/1.73 m2 was 5.5% for E and 13.8% for C. The corresponding figures for E less than 80 ml/min/1.73 m2 was 7.8% for E and 20.8% for C. Data in the literature on the inaccuracies of C and E versus GFR suggest that the degree of inaccuracy in predicting GFR from C is much higher than that from E, a feature which together with the present findings on precision indicates that E is much more reliable than C for routine determination of GFR in children.
Language of Publication: English
Unique Identifier: 78074716

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MeSH Heading (Major): Creatinine|*ME; Edetic Acid|*DU; Glomerular Filtration Rate|*
MeSH Heading: Adolescence; Child; Child, Preschool; Chromium Radioisotopes|DU; Comparative Study; Human; Infant; Methods

Publication Type: JOURNAL ARTICLE
ISSN: 0036-5513
Country of Publication: NORWAY

Record 19 from database: MEDLINE
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Title: IgG platelet antibodies in EDTA-dependent pseudothrombocytopenia bind to platelet membrane glycoprotein IIb.
Author: Fiorin F; Steffan A; Pradella P; Bizzaro N; Potenza R; De Angelis V
Address: Servizio Immunotrasfusionale, Ospedale Civile, San Donà di Piave, Italy.
Source: Am J Clin Pathol, 1998 Aug, 110:2, 178-83
Abstract: EDTA-dependent pseudothrombocytopenia (PTCP) consists of an inappropriate low platelet count caused by autoantibodies present in the serum samples reacting with platelets only in EDTA-anticoagulated blood. By using immunoprecipitation and Western blot techniques, we studied the immunochemical specificity of platelet agglutinating autoantibodies in the serum samples of 10 patients with PTCP. Furthermore, to evaluate a possible role of PTCP-associated IgG autoantibodies in increased platelet turnover, we assayed the plasma glycocalicin (GC) level and calculated the GC index for every patient. Our results provide direct evidence that an epitope located on platelet membrane glycoprotein IIb is recognized by PTCP-associated IgG antibodies; moreover GC levels in patients with EDTA-dependent PTCP were similar to control levels, thus excluding an increased platelet turnover. We conclude that antiplatelet antibodies directed against platelet cryptantigens are unlikely to have a major role in the increased removal of cells from circulation.
Language of Publication: English
Unique Identifier: 98368314

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MeSH Heading (Major): Autoantibodies|*IM; Blood Platelets|*IM; Edetic Acid|*; IgG|*IM; Platelet Glycoprotein GPIIb-IIIa Complex|*ME; Thrombocytopenia|*BL/*CI
MeSH Heading: Antibodies, Monoclonal|IM; Blotting, Western; Fluorescent Antibody Technique; Human; Platelet Count; Platelet Glycoprotein GPIb-IX Complex|AN; Precipitin Tests

Publication Type: JOURNAL ARTICLE
ISSN: 0002-9173
Country of Publication: UNITED STATES
CAS Registry/EC Number: 0 (glycocalicin); 0 (Antibodies, Monoclonal); 0 (Autoantibodies); 0 (IgG); 0 (Platelet Glycoprotein GPIb-IX Complex); 0 (Platelet Glycoprotein GPIIb-IIIa Complex); 60-00-4 (Edetic Acid)

Record 20 from database: MEDLINE
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Title: Metrizamide in urography. II. A comparison of 51Cr-EDTA clearance and metrizamide clearance in man.
Author: Golman K; Almen T; Denneberg T; Nosslin B
Address
Source: Invest Radiol, 1977 Jul-Aug, 12:4, 353-6
Abstract: In nine subjects undergoing urography with metrizamide measurements of total serum clearance of 51Cr-EDTA have been made before, during, and after the urography. During the urography both total serum clearances and renal clearances were determined for 51Cr-EDTA and metrizamide. The present study in man confirms the previous results from investigations in rabbits, that most of the intravenously injected metrizamide is excreted through the kidneys, that tubular reabsorption of metrizamide occurs and suggests that metrizamide might be used with advantage for urography.
Language of Publication: English
Unique Identifier: 77227155

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MeSH Heading (Major): Contrast Media|*ME; Edetic Acid|*ME; Iodobenzoates|*ME; Metrizamide|*ME; Urography|*
MeSH Heading: Chromium Radioisotopes; Comparative Study; Human

Publication Type: JOURNAL ARTICLE
ISSN: 0020-9996
Country of Publication: UNITED STATES

Record 21 from database: MEDLINE
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Title: Fe(III)-EDTA complex as iron fortification.
Author: Layrisse M; Martínez-Torres C
Address
Source: Am J Clin Nutr, 1977 Jul, 30:7, 1166-74
Abstract: Fe(III)-EDTA as iron fortification presents several advantages over the other iron salts previously used including ferrous sulfate. This iron compound exchange completely with vegetable food iron in the lumen of the gut but with the characteristics that the absorption from both, extrinsic and intrinsic food iron, is higher than that expected from other iron salfs. The comparison between the iron absorption from Fe(III)-EDTA and ferrous sulfate as iron fortification indicates that the absorption form EDTA is about twice as high than that observed from ferrous sulfate. The data indicates that only 10 to 15 mg of iron as Fe(III)-EDTA as iron fortification would be necessary to prevent iron deficiency anemia in population relying their subsistence of vegetable food only and free of parastic infection producing blood loss.
Language of Publication: English
Unique Identifier: 77219060

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MeSH Heading (Major): Edetic Acid|*AA/ME; Iron|*/ME
MeSH Heading: Absorption; Animal; Ascorbic Acid; Comparative Study; Corn; Female; Ferric Compounds; Ferrous Compounds; Food, Fortified; Hemoglobins|ME; Human; Male; Milk; Transferrin|ME

Publication Type: JOURNAL ARTICLE
ISSN: 0002-9165
Country of Publication: UNITED STATES

Record 22 from database: MEDLINE
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Title: Restoration of hemoglobin function in stored EDTA blood. Application in identification of hemoglobin variants with abnormal oxygen affinity.
Author: Sumoza A; Fairbanks VF; Pineda AA
Address
Source: Am J Clin Pathol, 1977 Jul, 68:1, 53-6
Abstract: The incubation of stored blood in a mixture of inosine, pyruvate, glucose, and phosphate restores the O2 affinity of hemoglobin to physiologic levels, as measured by the configuration of the dissociation curve and the P50. This regeneration of normal hemoglobin function not only is consistent for samples anticoagulated with EDTA and stored eight days at 4 C but also is demonstrable for at least 19 days of 4 C storage of EDTA-anticoagulated whole blood. This regeneration procedure is simple to perform and makes it possible to measure reliably O2 affinity in blood samples transmitted by mail.
Language of Publication: English
Unique Identifier: 77199151

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MeSH Heading (Major): Blood Preservation|*; Edetic Acid|*; Hemoglobins|*PH
MeSH Heading: Diphosphoglyceric Acids|BL; Female; Hemoglobins, Abnormal; Human; Male; Oxygen|BL; Oxygen Consumption

Publication Type: JOURNAL ARTICLE
ISSN: 0002-9173
Country of Publication: UNITED STATES

Record 23 from database: MEDLINE
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Title: GFR measurement with iohexol and 51Cr-EDTA. A comparison of the two favoured GFR markers in Europe.
Author: Brändström E; Grzegorczyk A; Jacobsson L; Friberg P; Lindahl A; Aurell M
Address: Department of Clinical Physiology, Sahlgrenska University Hospital, Göteborg, Sweden.
Source: Nephrol Dial Transplant, 1998 May, 13:5, 1176-82
Abstract: BACKGROUND: The aim of the present study was to compare the most commonly used GFR markers for clearance measurements, 51Cr-EDTA and iohexol, using two different methods for iohexol analysis, HPLC and X-ray fluorescence, referring both to the multiple-sample and single-sample calculations, using 51Cr-EDTA as the reference method. METHODS: Forty-nine patients with an estimated GFR >40 ml/min were included. 51Cr-EDTA and iohexol were injected simultaneously and blood samples were taken 150, 195 and 240 min after injection of the respective marker. RESULTS: The multiple-point clearances, determined from HPLC and X-ray fluorescence, compared to 51Cr-EDTA correlated highly (r=0.92 and 0.95 respectively). The results from single-point clearance comparison, iohexol measured by HPLC vs 51Cr-EDTA, yielded a correlation of r=0.91, while single-point clearance from iohexol, analysed by X-ray fluorescence, obtained a correlation of 0.93 and an intercept statistically different from origo. CONCLUSIONS: Iohexol and 51Cr-EDTA are comparable as GFR markers for multiple-point clearance measurements. The single-sample method for GFR >40 ml/min can be used with a high accuracy. The precision and accuracy of X-ray fluorescence analysis of low concentrations of iohexol were less than those of HPLC. Care should therefore be taken when using X-ray fluorescence that the injected dose of iohexol should result in a plasma concentration level of iodine of at least 0.06 mg/ml at the time of blood sampling.
Language of Publication: English
Unique Identifier: 98285008

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MeSH Heading (Major): Edetic Acid|*DU; Glomerular Filtration Rate|*/PH; Iohexol|*DU
MeSH Heading: Adult; Aged; Aged, 80 and over; Biological Markers|AN; Chromatography, High Pressure Liquid; Chromium Radioisotopes|DU; Comparative Study; Europe; Evaluation Studies; Female; Human; Male; Middle Age; Spectrometry, X-Ray Emission

Publication Type: JOURNAL ARTICLE
ISSN: 0931-0509
Country of Publication: ENGLAND
CAS Registry/EC Number: 0 (Biological Markers); 0 (Chromium Radioisotopes); 60-00-4 (Edetic Acid); 66108-95-0 (Iohexol)

Record 24 from database: MEDLINE
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Title: Tissue inhibitor of metalloproteinase-2 protection of matrix metalloproteinase-2 from degradation by plasmin is reversed by divalent cation chelator EDTA and the bisphosphonate alendronate.
Author: Farina AR; Tacconelli A; Teti A; Gulino A; Mackay AR
Address: Department of Experimental Medicine, University of L'Aquila, Italy. farina@aquila.infn.it
Source: Cancer Res, 1998 Jul 15, 58:14, 2957-60
Abstract: The degradation of tissue inhibitor of metalloproteinase (TIMP)-free matrix metalloproteinase (MMP)-2 to proteolytically inactive fragments by plasmin was inhibited in equimolar mixtures of purified TIMP-2 and TIMP-free MMP-2 and was not observed in purified MMP-2-TIMP-2 complexes. Divalent cation chelators EDTA and sodium Alendronate did not inhibit plasmin degradation of TIMP-free MMP-2 but reversed the ability of TIMP-2 to protect MMP-2 from degradation by plasmin. Our data confirm a role for plasmin in the clearance of TIMP-free MMP-2, identify a pivotal role for TIMP-2 in regulating MMP-2 longevity in plasmin-containing environments, and highlight a novel therapeutic use for chelators of divalent cations, including the bisphosphonate Alendronate, in the reversal of TIMP-2 protection of MMP-2 from degradation by plasmin. We propose that these observations are relevant to pathologies that are dependent upon plasmin and MMP-2 activity (e.g., tumor invasion and metastasis).
Language of Publication: English
Unique Identifier: 98343547

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MeSH Heading (Major): Gelatinases|*DE/ME; Metalloproteinases|*DE/ME; Plasmin|*AI/ME; Protease Inhibitors|*PD; Tissue Inhibitor-of Metalloproteinase-2|*PD
MeSH Heading: Alendronate|PD; Cations; Chelating Agents|PD; Diphosphonates|PD; Edetic Acid|PD; Human; Support, Non-U.S. Gov't; Tumor Cells, Cultured|DE

Publication Type: JOURNAL ARTICLE
ISSN: 0008-5472
Country of Publication: UNITED STATES
CAS Registry/EC Number: EC 3.4.21.7 (Plasmin); EC 3.4.24 (Metalloproteinases); EC 3.4.24.24 (gelatinase A); EC 3.4.99.- (Gelatinases); 0 (Cations); 0 (Chelating Agents); 0 (Diphosphonates); 0 (Protease Inhibitors); 127497-59-0 (Tissue Inhibitor-of Metalloproteinase-2); 60-00-4 (Edetic Acid); 66376-36-1 (Alendronate)

Record 25 from database: MEDLINE
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Title: Effects of smoking on the urine excretion of oral 51Cr EDTA in ulcerative colitis.
Author: Benoni C; Prytz H
Address: Department of Medicine, University Hospital of MalmÂo, Sweden.
Source: Gut, 1998 May, 42:5, 656-8
Abstract: BACKGROUND: Smokers have a reduced risk and ex-smokers an increased risk of ulcerative colitis (UC). Stopping smoking often precedes onset and relapses. Smoking reduces the 24 hour urine excretion of oral chromium-51 labelled EDTA in healthy individuals. AIMS: To estimate the effects of smoking on the urine excretion of oral 51Cr EDTA in well characterised patients with UC. SUBJECTS: Sixteen smoking and 16 non-smoking patients with UC in remission were studied. The non-smokers had never smoked. Most were taking 5-aminosalicylic acid. No patient took steroids or immunosuppressants. The control group comprised 25 smoking healthy volunteers and 25 who had never smoked. The median cigarette consumption was equal in the patients and volunteers. METHODS: The 24 hour urine excretion of oral 51Cr EDTA was measured and the results were correlated with smoking habits, number of cigarettes, and disease extent. RESULTS: Patients with UC had significantly higher 24 hour urine recoveries than healthy controls (p = 0.04). This difference was more pronounced when patients who smoked were compared with healthy smokers (p = 0.005) No significant differences were found when comparing non-smoking patients with non-smoking controls or when comparing smoking and non-smoking patients. Urine recoveries did not correlate with number of cigarettes or disease extent. Smoking was more prevalent in patients with a more limited disease extent (p = 0.033). CONCLUSIONS: Effects of smoking on the urine excretion of 51Cr EDTA in health were abolished by the presence of UC. The protective effects of smoking in established UC are not due to a moderating effect of smoking on intestinal permeability.
Language of Publication: English
Unique Identifier: 98323295

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MeSH Heading (Major): Colitis, Ulcerative|*UR; Edetic Acid|*ME; Smoking|*UR
MeSH Heading: Adult; Aged; Chromium Radioisotopes|ME; Female; Human; Intestinal Absorption; Male; Middle Age; Support, Non-U.S. Gov't

Publication Type: JOURNAL ARTICLE
ISSN: 0017-5749
Country of Publication: ENGLAND

Record 26 from database: MEDLINE
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Title: Ultrasonic subgingival root planing and EDTA etching in a one-step procedure.
Author: Blomlöf J; Blomlöf L; Lindskog S
Address: Department of Basic Oral Sciences, School of Dentistry, Karolinska Institutet, Huddinge, Stockholm, Sweden.
Source: Swed Dent J, 1997, 21:6, 213-9
Abstract: The purpose of the present study was to investigate if ultrasonic debridement and EDTA etching could be combined in a ore-step procedure. Tap water was exchanged for a 24% EDTA solution as irrigation agent during root planing with a piezo scaler. 24 human teeth extracted due to severe periodontal disease were used and the results were evaluated with scanning electron microscopy. Results indicate that synergistic effects (smear removal and exposure of collagen fibers) were negligible with conventional application of the irrigation fluid to the working area of the scaling tip while a marked effect was evident when the EDTA solution was applied directly to the working area of the piezoelectric scaler through a customized tip.
Language of Publication: English
Unique Identifier: 98166232

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MeSH Heading (Major): Acid Etching, Dental|*MT; Chelating Agents|AD/*TU; Edetic Acid|AD/*TU; Root Planing|*/IS/MT; Subgingival Curettage|*/IS/MT; Ultrasonic Therapy|*/IS/MT
MeSH Heading: Collagen|UL; Dental Cementum|UL; Dental Scaling|IS/MT; Dentin|UL; Human; Irrigation; Microscopy, Electron, Scanning; Periodontitis|TH; Water

Publication Type: CLINICAL TRIAL; JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
ISSN: 0347-9994
Country of Publication: SWEDEN

Record 27 from database: MEDLINE
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Title: Lead exposure and accumulation in healthy Thais: assessed by lead levels, EDTA mobilization and heme synthesis-related parameters.
Author: Wananukul W; Sirivarasai J; Sriapha C; Chanatara V; Chunvimaluang N; Keanpoompuang A; Boriboon W; Pumala K; Kaojarern S
Address: Department of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok Thailand.
Source: J Med Assoc Thai, 1998 Feb, 81:2, 110-6
Abstract: Lead is one of the pollutants which is of public concern. The magnitude of lead contamination in Thai people is of interest. The objective of this study was to evaluate the lead status in normal healthy volunteers. Normal volunteers were included. The blood for lead level, Zinc protoporphyrin (ZPP), delta-aminolevulinic acid dehydratase (ALA-D) activity, and baseline urine for lead, delta-aminolevulinic acid (ALA) and coproporphyrinogen III (CP3) were collected. The EDTA mobilization test was done. 24 hour urine after administration of the drug was collected for lead analysis. Thirty volunteers were included in the study. All were men whose average age was 32.5 +/- 6.9 years. The mean lead level was 5.95 +/- 2.01 micrograms/dL and 5.83 +/- 2.32 micrograms/L in urine. The 24 hour urine lead contents before and after EDTA administration were significantly different (11.11 +/- 6.72 and 16.05 +/- 9.51 micrograms respectively). Blood ALA-D activity was 251.6 +/- 80.4 unit/ml of RBC. Urine ALA and CP3 were 0.56 +/- 1.2 mg/L and 22.17 +/- 23.9 micrograms/L respectively. All were in the normal ranges. All parameters suggested that the healthy Thai volunteers had an acceptable magnitude of lead exposure and accumulation.
Language of Publication: English
Unique Identifier: 98190745

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MeSH Heading (Major): Edetic Acid|DU/*ME; Environmental Exposure|AE/*AN; Environmental Monitoring|*; Heme|*BI/ME; Lead|AN/*BL/*UR
MeSH Heading: Adult; Creatinine|AN/UR; Human; Male; Middle Age; Reference Values; Support, Non-U.S. Gov't; Thailand; Urinalysis

Publication Type: CLINICAL TRIAL; JOURNAL ARTICLE
ISSN: 0125-2208
Country of Publication: THAILAND

Record 28 from database: MEDLINE
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Title: Defining the orientation of the human U1A RBD1 on its UTR by tethered-EDTA(Fe) cleavage.
Author: Beck DL; Stump WT; Hall KB
Address: Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Source: RNA, 1998 Mar, 4:3, 331-9
Abstract: The N-terminal RNA binding domain of the human U1A protein (RBD1) specifically binds an RNA hairpin of U1 snRNA as well as two internal loops in the 3' UTR of its own mRNA. Here, a single cysteine has been introduced into Loop 1 of RBD1, which is subsequently used to attach (EDTA-2-aminoethyl) 2-pyridyl disulfide-Fe3+ (EPD-Fe). This EDTA-Fe derivative is used to generate hydroxyl radicals to cleave the proximal RNA sugar-phosphate backbone in the RNA-RBD complexes. RBD1(K20C)-EPD-Fe cleaves the 5' strand of the RNA hairpin stem, centered four base pairs away from the base of the loop, and cleaves the UTR in two places, again centered on the 5' side of the fourth base pair from each internal loop. These data, extrapolated to the position of Lys 20 in RBD1, orient the two proteins bound to the UTR, and provide direct biochemical evidence for the proposed model of the RBD1:UTR complex.
Language of Publication: English
Unique Identifier: 98169154

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MeSH Heading (Major): Ribonucleoproteins, Small, U1|CH/*GE/*ME; RNA|CH/*ME; Translation, Genetic|*
MeSH Heading: Base Sequence; Binding Sites; Edetic Acid|AA/CH/ME; Human; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Nucleic Acid Conformation; Protein Conformation; RNA-Binding Proteins|CH/GE/ME; RNA, Small Nuclear|ME; Support, Non-U.S. Gov't; Support, U.S. Gov't, P.H.S.

Publication Type: JOURNAL ARTICLE
ISSN: 1355-8382
Country of Publication: UNITED STATES

Record 29 from database: MEDLINE
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Title: Essential monoclonal gammopathy with an IgM paraprotein that is a cryoglobulin with cold agglutinin and EDTA-dependent platelet antibody properties.
Author: Pujol M; Ribera JM; Jimenez C; Ribera A; Abad E; Feliu E
Address: Servicio de HematologÆia y Hemoterapia del Hospital Universitario Germans Trias y Pujol, Badalona, Spain.
Source: Br J Haematol, 1998 Mar, 100:3, 603-4
Abstract: A patient with apparent anaemia and thrombocytopenia caused by a monoclonal paraprotein is described. The patient's serum contained a monoclonal IgM kappa, a cryoglobulin and a cold agglutinin. The cryoglobulin, similar to the serum paraprotein, was a monoclonal IgM kappa. Serum was studied to determine the relationship of the cryoglobulin with the cold agglutinin. The cryoglobulin and cold agglutinin were found to be the same paraprotein. Moreover, with absorption and elution techniques the reactivity of the autoantibody with both erythrocytes and platelets was demonstrated. Reports of cryoprecipitable cold agglutinins are rare and therefore this case is exceptional given that the IgM kappa paraprotein was found to be a cold agglutinin which was also reactive with platelets.
Language of Publication: English
Unique Identifier: 98163178

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MeSH Heading (Major): Cryoglobulins|*IM; IgM|*BL; Paraproteinemias|*IM
MeSH Heading: Aged; Anemia, Hemolytic, Autoimmune|IM; Autoantibodies|IM; Blood Platelets|IM; Case Report; Edetic Acid; Erythrocytes|IM; Female; Human; Thrombocytopenia|IM

Publication Type: JOURNAL ARTICLE
ISSN: 0007-1048
Country of Publication: ENGLAND

Record 30 from database: MEDLINE
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Title: The role of EDTA in provoking allergic reactions to subcutaneous infusion of apomorphine in patients with Parkinson's disease: a histologic study.
Author: van Laar T; van Hilten B; Neef C; Rutgers AW; Pavel S; Bruijn JA
Address: Department of Neurology, University Hospital Leiden.
Source: Mov Disord, 1998 Jan, 13:1, 52-5
Abstract: One of the formulations of apomorphine, used in clinical practice, contains sodium edetate (EDTA). EDTA is a chelator which indirectly prevents oxidation of apomorphine. A clinical and histologic study in four patients revealed that apomorphine with EDTA caused severe subcutaneous nodules, histologically characterized by an inflammatory infiltrate with a large amount of eosinophils, indicating a cell-mediated allergic reaction. After withdrawal of EDTA, this allergic component completely disappeared, which was accompanied clinically by less extensive nodule formation with a softer consistency. It is therefore recommended that EDTA be excluded from apomorphine formulations.
Language of Publication: English
Unique Identifier: 98112705

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MeSH Heading (Major): Abdominal Muscles|*PA; Antiparkinson Agents|*AE; Apomorphine|*AE; Chelating Agents|*AE; Drug Eruptions|*PA; Edetic Acid|*AE
MeSH Heading: Drug Combinations; Female; Human; Injections, Subcutaneous; Intervention Studies; Longitudinal Studies; Male; Middle Age; Vasculitis, Allergic Cutaneous|CI/PA

Publication Type: JOURNAL ARTICLE
ISSN: 0885-3185
Country of Publication: UNITED STATES

Record 31 from database: MEDLINE
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Title: EDTA chelation therapy should be more commonly used in the treatment of vascular disease.
Author: Chappell LT
Address: Wright State School of Medicine, USA.
Source: Altern Ther Health Med, 1995 May, 1:2, 53-7
Abstract: EDTA chelation therapy is safe, effective, and more economical than commonly used surgical treatments for vascular disease. This article includes evidence of effectiveness, mechanisms of action of EDTA, a discussion of studies that have been done regarding the therapy, and some brief case reports. The conclusion is that EDTA chelation therapy should be a therapeutic option for vascular disease, either by itself or in conjunction with standard protocols.
Language of Publication: English
Unique Identifier: 98081084

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MeSH Heading (Major): Chelation Therapy|*; Peripheral Vascular Diseases|*TH
MeSH Heading: Human; Treatment Outcome

Publication Type: JOURNAL ARTICLE
ISSN: 1078-6791
Country of Publication: UNITED STATES

Record 32 from database: MEDLINE
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Title: Fibrinogen present in EDTA--anticoagulated plasma stimulates the tissue-type plasminogen activator-catalysed conversion of plasminogen to plasmin.
Author: Haddeland U; Bennick A; Brosstad F
Address: Research Institute for Internal Medicine, University of Oslo, Rikshospitalet, Norway.
Source: Blood Coagul Fibrinolysis, 1994 Oct, 5:5, 767-72
Abstract: The presence of soluble fibrin in plasma is an early and sensitive indicator of activation of the coagulation system. Quantitative spectrophotometric assays for soluble fibrin can be based on the principle that soluble fibrin stimulates the tissue-type plasminogen activator-catalysed conversion of plasminogen to plasmin. It was previously shown that treatment of purified fibrinogen by EDTA, which removes the three tightly bound Ca2+ ions, results in exposure of tissue-type plasminogen activator-catalytic sites similar to those unveiled by thrombin. Since EDTA is a common anticoagulant, it was of interest to study the effect of EDTA on a test based on plasminogen activation. It is concluded that the determination of soluble fibrin in EDTA-anticoagulated plasma from healthy individuals gives a false positive indication of the presence of soluble fibrin. This was true irrespective of whether the test was performed at pH 7.4, 7.8 or 8.5. The most probable explanation is that tissue-type plasminogen activator-stimulating sites are exposed in fibrinogen by EDTA. Therefore, EDTA-plasma is unsuitable for assaying soluble fibrin with tests based on the tissue-type plasminogen activator-mediated conversion of plasminogen to plasmin.
Language of Publication: English
Unique Identifier: 95169887

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MeSH Heading (Major): Edetic Acid|*; Fibrinogen|*PD; Plasmin|*ME; Plasminogen|*ME; Tissue Plasminogen Activator|*ME
MeSH Heading: Amino Acid Sequence; Anticoagulants; Human; Hydrogen-Ion Concentration; Molecular Sequence Data; Support, Non-U.S. Gov't

Publication Type: JOURNAL ARTICLE
ISSN: 0957-5235
Country of Publication: ENGLAND

Record 33 from database: MEDLINE
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Title: Freeze-dried fibrinogen or fibrinogen in EDTA stimulate the tissue-type plasminogen activator-catalysed conversion of plasminogen to plasmin.
Author: Haddeland U; Sletten K; Bennick A; Brosstad F
Address: Research Institute for Internal Medicine, University of Oslo, Rikshospitalet, Norway.
Source: Blood Coagul Fibrinolysis, 1994 Aug, 5:4, 575-81
Abstract: Both soluble and insoluble fibrin stimulate the tissue-type plasminogen activator-catalysed conversion of plasminogen to plasmin. Whether fibrinogen can exert a similar effect has been a controversial issue. The present investigation shows that while fibrinogen purified by beta-alanine precipitation does not stimulate the tissue-type plasminogen activator-catalysed plasminogen activation, fibrinogen which has been either lyophilized or stripped of bound Ca2+ ions by EDTA chelation, stimulates this reaction. The data indicate that such procedures alter the molecular conformation of fibrinogen, and expose stimulatory sites which are hidden in the native fibrinogen molecule. These results may explain previous findings concerning the capacity of fibrinogen as a stimulator of the tissue-type plasminogen activator-catalysed plasminogen activation. Since even slight alteration of the molecular structure of fibrinogen leads to an increase in the tissue-type plasminogen activator stimulation, the authors suggest that this can be used to test if the fibrinogen is in a native state.
Language of Publication: English
Unique Identifier: 95143398

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MeSH Heading (Major): Fibrinogen|*CH/PH; Plasmin|*BI; Plasminogen|*ME; Tissue Plasminogen Activator|*ME
MeSH Heading: beta-Alanine; Amino Acid Sequence; Comparative Study; Edetic Acid; Freeze Drying; Human; Molecular Sequence Data; Precipitation; Protein Conformation; Solubility; Support, Non-U.S. Gov't; Thrombin|ME

Publication Type: JOURNAL ARTICLE
ISSN: 0957-5235
Country of Publication: ENGLAND

Record 34 from database: MEDLINE
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Title: An EDTA-associated anti-B agglutinin: the role of ionized calcium.
Author: Yasuda H; Ohto H; Motoki R; Uchikawa M
Address: Blood Transfusion Service, Fukushima Medical College, Japan.
Source: Transfusion, 1997 Nov, 37:11-12, 1131-6
Abstract: BACKGROUND: It is believed that EDTA-dependent panagglutination is associated with free carboxylic acids that support reactions of rare autoagglutinins. CASE REPORT: An ABO typing discrepancy occurred in an 88-year-old patient. The specificity of his autoagglutinin was demonstrated by panel cell study and absorption tests using normal donors' red cells or immunoadsorbents coated with A, B, or O substances. Inhibition assays were performed to determine whether the autoagglutinin was inhibited by ionized calcium or carboxylic acids. The autoagglutinin had anti-B specificity when tested in the presence of EDTA. It was neutralized by group B secretor saliva and adsorbed by crystalline silica coated with simple B substances with or without EDTA, although it was absorbed by group B red cells only in the presence of EDTA. The agglutinating activity was stronger at 25 degrees C (titer 64) than at 37 degrees C (titer 16) and was destroyed by treatment of the serum with dithiothreitol, which suggests that the autoagglutinin is IgM. This activity also appeared in the patient's serum after dialysis and in an eluate obtained after adsorption with simple B substances, and it was inhibited by the addition of CaCl2 at 0.5 mM or higher concentrations. This suggests that the agglutination is not dependent on EDTA but, rather, on the concentration of ionized calcium. The autoagglutinin failed to react with group B red cells treated with glutaraldehyde for 10 minutes. CONCLUSION: An anti-B autoagglutinin was shown to have caused an ABO typing discrepancy in the presence of EDTA. These results suggest that autoagglutination requires an environment with low levels of ionized calcium, but not the presence of carboxyl groups.
Language of Publication: English
Unique Identifier: 98088041

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MeSH Heading (Major): Agglutinins|*DE/IM; ABO Blood-Group System|*IM; Edetic Acid|*PD
MeSH Heading: Aged; Aged, 80 and over; Agglutination Tests; Calcium|IM/PH; Case Report; Cations|IM/PD; Cell-Free System|DE; Disaccharides|PK; Erythrocytes|DE/IM/ME; Human; Hydrogen-Ion Concentration; Immunosorbent Techniques; Isoantibodies|BL/IM; Male; Neutralization Tests; Trisaccharides|PK

Publication Type: JOURNAL ARTICLE
ISSN: 0041-1132
Country of Publication: UNITED STATES

Record 35 from database: MEDLINE
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Title: Aminoglycosides prevent and dissociate the aggregation of platelets in patients with EDTA-dependent pseudothrombocytopenia.
Author: Sakurai S; Shiojima I; Tanigawa T; Nakahara K
Address: Department of Central Laboratory, Tokyo University Hospital, Japan.
Source: Br J Haematol, 1997 Dec, 99:4, 817-23
Abstract: Although EDTA-dependent pseudothrombocytopenia (EDTA-PTCP) is of practical importance because failure to recognize this clinical entity may result in misdiagnosis and subsequent mismanagement of the patients, the pathophysiological nature of EDTA-PTCP remains unknown. To develop an effective way to evaluate the platelet counts in patients with EDTA-PTCP, we introduced aminoglycosides-supplemented anticoagulating agents. When kanamycin was pre-supplemented with EDTA for anticoagulating blood samples from EDTA-PTCP patients there was no significant change in the platelet counts and the morphology of blood cells after 150 min of incubation at room temperature. Furthermore, when kanamycin was added to EDTA-anticoagulated blood samples from EDTA-PTCP patients within 30 min after blood withdrawal, rapid dissociation of platelets without apparent morphological changes of blood cells was observed, and complete blood cell counts as well as the histogram patterns were almost the same as those examined immediately after blood sampling. The dissociation of aggregated platelets was also detected when other antibiotics were used, although it was associated with some extent of morphological changes of blood cells. These findings indicate that the supplementation of aminoglycosides either before or after blood sampling is a useful method for the diagnosis EDTA-PTCP and for the evaluation of platelet counts in patients with EDTA-PTCP.
Language of Publication: English
Unique Identifier: 98092324

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MeSH Heading (Major): Antibiotics, Aminoglycoside|*TU; Edetic Acid|*AE; Kanamycin|*TU; Platelet Aggregation|*DE; Thrombocytopenia|*BL/CI
MeSH Heading: Adolescence; Adult; Aged; Aged, 80 and over; Female; Human; Male; Middle Age; Platelet Count

Publication Type: JOURNAL ARTICLE
ISSN: 0007-1048
Country of Publication: ENGLAND

Record 36 from database: MEDLINE
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Title: Iron release from recombinant N-lobe and single point Asp63 mutants of human transferrin by EDTA.
Author: He QY; Mason AB; Woodworth RC
Address: Department of Biochemistry, College of Medicine, University of Vermont, Burlington, VT 05405, USA.
Source: Biochem J, 1997 Dec, 328 ( Pt 2):, 439-45
Abstract: Transferrins bind ferric ion and deliver the iron to cells. The mechanism of the iron release has been studied kinetically, in vitro, with the aid of single point mutants in which the iron-binding ligand, Asp63 (aspartic acid-63, D63), has been changed to Ser, Asn, Glu and Ala. Iron release from the unmutated N-lobe of human serum transferrin (hTF/2N) by EDTA is influenced by a variety of factors. The rate-determining conformational-change mechanism may be a major pathway for iron release from hTF/2N's having a 'closed' conformation, which leads to a saturation kinetic mode with respect to ligand concentration. The effect of chloride depends on the protein conformation, showing a negative action in the case of tight binding and a positive action when the protein has an 'open' or 'loose' conformation. The negative effect of chloride could originate from the binding competition between chloride and the chelate to the active site for iron release, and the positive effect could derive from the synergistic participation of chloride in iron removal. The 'open' conformation may be induced by decreasing pH: the transitional point appears to be at about pH 6.3 for the wild-type hTF/2N; the 'loose' conformation may be facilitated by mutations at D63, which result in the loss of a key linking component in interdomain interactions of the protein. In the latter case, structural factors dominate over other potential negative effects because the weak interdomain contacts derived from the mutation of D63 cause the binding site to open easily, even at pH 7.4. Therefore chloride exhibits an accelerating action on iron release by EDTA from all the D63 mutants.
Language of Publication: English
Unique Identifier: 98041883

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MeSH Heading (Major): Aspartic Acid|*GE; Iron|*ME; Point Mutation|*; Transferrin|CH/GE/*ME
MeSH Heading: Binding, Competitive; Comparative Study; Edetic Acid|PD; Human; Hydrogen-Ion Concentration; Peptide Fragments|GE/ME; Potassium Chloride|PD; Protein Conformation; Recombinant Proteins|CH/ME; Support, Non-U.S. Gov't; Support, U.S. Gov't, P.H.S.

Publication Type: JOURNAL ARTICLE
ISSN: 0264-6021
Country of Publication: ENGLAND

Record 37 from database: MEDLINE
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Title: Assessment of glomerular filtration rate utilizing subcutaneously injected 51Cr-EDTA.
Author: Monteiro MC; Alonso G; Ajzen H; Pereira AB
Address: Disciplinas de Nefrologia e Medicina Nuclear, Escola Paulista de Medicina, SÃao Paulo, Brasil.
Source: Braz J Med Biol Res, 1994 Nov, 27:11, 2557-64
Abstract: 1. 51Cr-EDTA injected with lidocaine and epinephrine, as a subcutaneous button, is slowly absorbed, and a plasma level that is relatively stable can be maintained for a time sufficient to permit measurement of the renal clearance of EDTA, which is a measure of glomerular filtration rate (GFR). We studied this procedure in 32 normal volunteers and 24 patients with different glomerulopathies, comparing EDTA and creatinine clearances. In 20 patients these measurements were also compared with inulin clearance. 2. Creatinine clearance overestimates GFR due to tubular secretion of creatinine. This secretion is present even in patients with significantly reduced glomerular filtration rates. As a consequence, the lower the GFR the higher the overestimation will be. 3. A good correlation was obtained between the 51Cr-EDTA and inulin clearance: y(EDTA) = 4.21 + 0.88 x (inulin), r = 0.98. The procedure is simple to perform, and the radiotracer utilized is significantly less expensive than iothalamate.
Language of Publication: English
Unique Identifier: 96002402

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MeSH Heading (Major): Chromium Radioisotopes|AD/BL/*DU; Edetic Acid|AD/AN/*DU; Glomerular Filtration Rate|*
MeSH Heading: Adolescence; Adult; Analysis of Variance; Comparative Study; Creatinine|BL; Epinephrine|AD/PD; Female; Human; Injections, Subcutaneous; Inulin|AD/BL; Lidocaine|AD/PD; Male; Middle Age; Support, Non-U.S. Gov't; Time Factors

Publication Type: CLINICAL TRIAL; JOURNAL ARTICLE
ISSN: 0100-879X
Country of Publication: BRAZIL

Record 38 from database: MEDLINE
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Title: The analysis of EDTA in dried bloodstains by electrospray LC-MS-MS and ion chromatography.
Author: Miller ML; McCord BR; Martz R; Budowle B
Address: Forensic Science Research and Training Center, FBI Laboratory, Quantico, Virginia 22135, USA.
Source: J Anal Toxicol, 1997 Nov, 21:7, 521-8
Abstract: Analytical methods were developed to determine the presence of ethylenediaminetetraacetic acid (EDTA) in dried bloodstains to provide probative information when allegations of evidence tampering have been made in criminal cases. A simple screening method using ion chromatography to analyze stains was found to be quantitative to the 5 ppm level. The presence of EDTA was then confirmed using negative and positive ion mode liquid chromatography-tandem mass spectrometry (LC-MS-MS) methods. A blind trial of these methods on 42 samples correctly determined the bloodstains that did and did not contain the preservative EDTA. One interesting observation in these results was the adsorption and postanalysis release of EDTA in the chromatographic system. In order to avoid cross contamination of samples resulting from this phenomena, it was found to be necessary to use EDTA-free blood extracts as blanks in the LC-MS analysis of bloodstains.
Language of Publication: English
Unique Identifier: 98061488

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MeSH Heading (Major): Blood Stains|*; Edetic Acid|*AN/CH
MeSH Heading: Chromatography, Ion Exchange; Chromatography, Liquid; Electrochemistry; Forensic Medicine; Human; Molecular Weight; Spectrum Analysis, Mass

Publication Type: JOURNAL ARTICLE
ISSN: 0146-4760
Country of Publication: UNITED STATES

Record 39 from database: MEDLINE
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Title: Human nasal absorption of 51Cr-EDTA in smokers and control subjects.
Author: Greiff L; Wollmer P; Andersson M; Persson CG
Address: Department of Otorhinolaryngology, University Hospital, Lund, Sweden.
Source: Clin Exp Allergy, 1994 Nov, 24:11, 1036-40
Abstract: Passive exposure to cigarette smoke has emerged as a significant risk factor in the development of asthma and allergic airways disease. The pathogenetic mechanisms are not known, but increased absorption across the airway epithelial lining has been suggested as one possible mechanism of this effect of cigarette smoke. This study examines the absorption-permeability of the nasal epithelial lining in cigarette smokers and non-smokers. For comparison, the effect of a detergent, dioctylsodium sulfosuccinate (DS), is also examined. A solution containing 51Cr-EDTA (51-chromium labeled ethylene diamine tetraacetic acid) (mol. wt. 372 Da) was instilled and maintained in the nasal cavity in six smokers and 12 non-smokers for 15 min. Urine was collected for 24 h after the instillation. The accumulated amount of excreted 51Cr-EDTA was measured and expressed as millilitre nasal instillate. In six non-smokers the procedure was repeated when DS has been added to the instillate. The median recovered amount of 51Cr-EDTA in smokers 0.07 ml (range 0.04-0.32) was not significantly different from that in non-smokers 0.16 ml (0.01-1.22). The recovered amount of 51Cr-EDTA increased from a median of 0.18 ml (0.01-1.22) to 1.13 ml (0.53-1.80) after addition of the detergent (P = 0.028). We conclude that the nasal airway absorption-permeability is not increased in smokers. Hence, passive exposure to cigarette smoke may not produce an impairment of airway barrier functions.
Language of Publication: English
Unique Identifier: 95179649

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MeSH Heading (Major): Edetic Acid|*PK; Nasal Mucosa|DE/*ME; Smoking|*ME
MeSH Heading: Absorption|DE; Adult; Cathartics|PD; Cell Membrane Permeability|PH; Chromium Radioisotopes; Dioctyl Sulfosuccinic Acid|PD; Human; Male; Nasal Cavity|ME; Support, Non-U.S. Gov't; Tissue Distribution; Tobacco Smoke Pollution

Publication Type: JOURNAL ARTICLE
ISSN: 0954-7894
Country of Publication: ENGLAND

Record 40 from database: MEDLINE
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Title: Screening for EDTA-dependent deviations in platelet counts and abnormalities in platelet distribution histograms in pseudothrombocytopenia.
Author: Bartels PC; Schoorl M; Lombarts AJ
Address: Department of Clinical Chemistry, Haematology and Immunology, Medical Centre Alkmaar, The Netherlands.
Source: Scand J Clin Lab Invest, 1997 Nov, 57:7, 629-36
Abstract: Screening for pseudothrombocytopenia caused by in vitro platelet clumping has been performed in 45,000 subjects attending a general hospital. In our region, the observed prevalence of EDTA-induced pseudothrombocytopenia in blood samples with an initial platelet count below 150 x 10(9)/l was estimated to amount to 0.1%. EDTA-induced pseudothrombocytopenia was confirmed by detection of platelet aggregates by means of microscopic evaluation from the blood smear. In routine investigations, pseudothrombocytopenia could be highly suspected when the Sysmex NE 8000 showed characteristic peculiarities in the white blood cell (WBC) scattergram and histogram. Platelet aggregation is avoided in such cases by the use of citrate as an anticoagulant instead of EDTA. Pseudothrombocytopenia was detected in 46 subjects. As a screening test for pseudothrombocytopenia, increased cut-off values derived from the WBC histogram demonstrated 90% sensitivity and 100% specificity. Automated flagging for platelet clumps, deviations reflecting MPV, or PDW abnormalities revealed lower scores with respect to sensitivity.
Language of Publication: English
Unique Identifier: 98059884

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MeSH Heading (Major): Chelating Agents|*; Diagnostic Errors|*; Edetic Acid|*; Platelet Count|*MT; Thrombocytopenia|*DI
MeSH Heading: Anticoagulants; Blood Platelets|CY; Cell Size; Citric Acid; Comparative Study; Human; Leukocyte Count; Platelet Aggregation

Publication Type: JOURNAL ARTICLE
ISSN: 0036-5513
Country of Publication: NORWAY

Record 41 from database: MEDLINE
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Title: Comparison of manual and automated cell counts in EDTA preserved synovial fluids. Storage has little influence on the results.
Author: Salinas M; Rosas J; Iborra J; Manero H; Pascual E
Address: Unit of Clinical Biochemistry, Hospital General Universitario de Alicante, Spain.
Source: Ann Rheum Dis, 1997 Oct, 56:10, 622-6
Abstract: OBJECTIVE: To determine the precision and agreement of synovial fluid (SF) cell counts done manually and with automated counters, and to determine the degree of variability of the counts in SF samples, kept in the tubes used for routine white blood cell (WBC) counts--which use liquid EDTA as anticoagulant--at 24 and 48 hours at 4 degrees C, and at room temperature. METHODS: To determine precision, cell counts were repeated 10 times--both manually and by an automated counter--in a SF sample of low, medium, and high cellularity. The variances were calculated to determine the interobserver variation in two manual (M1,M2) and two automated cell counts (C1,C2). The agreement between a manual (M1) and automated counter (C1) results, was analysed by the Bland and Altman method and the difference against the mean of the two methods was plotted. Then, the mean difference between the two methods was estimated and the standard deviation of the difference. To determine the effects of storage, SF samples were kept in a refrigerator at 4 degrees C, and at room temperature; cell counts were done manually (M1) and automatically (C1) at 24 and 48 hours and the changes analysed by the Bland and Altman method. The variances were compared using an F test. RESULTS: (1) Precision. With the manual technique, the coefficients of variation were 27.9%, 14%, and 10.7% when used for counting the SF with low (270), medium (6200), and high cellularities (25,000). With the automated technique the coefficients of variation were 20%, 3.4%, and 2.9% in the same SF samples. In the fluids of medium and high cellularity, the variances of the automated cell counts were significatively lower (F test, p < 0.002) than those of the manual counts. (2) Interobserver variation. The variance between C1 and C2 (25 SF) was significatively lower (F test, p < 0.002) than that of the manual counts (41 SF). (3) Agreement between the two techniques (100 SF). For cellularities above 2000 cells/mm3, the manual method gave results between +10% to -34% of the results obtained by the coulter. For cellularities below 2000 cells/mm3, manual cell counts were between +60 to -1280 cells/mm3 of those obtained by the automated counter. (4) Influence of storage. The coulter counts of SF samples preserved at 4 degrees C showed less variance (F test, p < 0.05) than the manual counts. The worst results were obtained in manual counts of SF samples kept at room temperature; these samples at 48 hours showed a variation between -47% to 42% of the initial results. CONCLUSIONS: Automated cell count of the SF offers advantages: it gives higher precision and consumes less time. The stability of the samples preserved in the EDTA tubes used for routine WBC counts is of additional interest, because if delay cannot be avoided, the results of the WBC counts are still accurate at 24 and even at 48 hours, at least for clinical purposes.
Language of Publication: English
Unique Identifier: 98050768

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MeSH Heading (Major): Synovial Fluid|*IM
MeSH Heading: Automatic Data Processing; Blood Preservation; Comparative Study; Edetic Acid; Human; Lymphocyte Count|MT; Observer Variation; Reproducibility of Results; Sensitivity and Specificity; Support, Non-U.S. Gov't; Temperature

Publication Type: JOURNAL ARTICLE
ISSN: 0003-4967
Country of Publication: ENGLAND

Record 42 from database: MEDLINE
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Title: Comparison of EDTA and acid-citrate-dextrose collection tubes for detection of cytomegalovirus antigenemia and infectivity in leukocytes before and after storage.
Author: Landry ML; Cohen S; Huber K
Address: Clinical Virology Laboratory, Yale New Haven Hospital, Connecticut 06504, USA.
Source: J Clin Microbiol, 1997 Jan, 35:1, 305-6
Abstract: Duplicate blood samples collected in EDTA and acid-citrate-dextrose (ACD) were compared by cytomegalovirus (CMV) pp65 antigenemia and CMV infectivity on the day of sample collection and after 1 and 2 days of storage at 4 degrees C. No significant difference was detected between EDTA and ACD. However, CMV antigenemia was more sensitive than culture at all time points tested.
Language of Publication: English
Unique Identifier: 97123687

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MeSH Heading (Major): Blood Preservation|*IS; Cytomegalovirus|*IP; Leukocytes|*VI
MeSH Heading: Citric Acid; Edetic Acid; Glucose|AA; Human; Support, Non-U.S. Gov't; Support, U.S. Gov't, P.H.S.; Viral Load

Publication Type: JOURNAL ARTICLE
ISSN: 0095-1137
Country of Publication: UNITED STATES

Record 43 from database: MEDLINE
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Title: Synergistic effect of gramicidin and EDTA in inhibiting sperm motility and cervical mucus penetration in vitro.
Author: Bourinbaiar AS; Lee CH
Address: Metatron, Inc, New York, NY 10003 USA. emballon@usa.pipeline.com
Source: Contraception, 1996 Dec, 54:6, 367-72
Abstract: Gramicidin, a linear polypeptide with antiviral and antimicrobial properties, was compared in vitro with a commonly used spermicidal detergent-nonoxynol-9 (N9). The inhibition of sperm functions was evaluated by computer-assisted semen analysis (CASA) for sperm motility, in cervical mucus penetration assay, and by colorimetric tetrazolium salt and lactate dehydrogenase release assays routinely employed for testing the toxicity of drugs. The effective 100% inhibitory concentration (IC100) of gramicidin in a 2-min sperm immobilization assay by CASA was equal to 4 micrograms/ml, whereas IC100 of N9 was equal to 200 micrograms/ml. The presence of 0.1% of chelating agent, EDTA, reduced IC100 of gramicidin to 10 ng/ml, while less than a twofold enhancement in N9 activity was observed upon combination with EDTA. Likewise, the gramicidin/EDTA combination was 100,000 times more potent than N9/EDTA in the sperm penetration assay. Quantitative toxicity tests confirmed that gramicidin is a potent spermostatic rather than spermicidal agent. Further development of a gramicidin/EDTA formulation is warranted as a nontoxic topical contraceptive with activity against viral and microbial sexually transmitted diseases (STDs).
Language of Publication: English
Unique Identifier: 97123418

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MeSH Heading (Major): Cervix Mucus|*; Edetic Acid|AD/*PD; Gramicidin|AD/*PD; Sperm Motility|*DE; Sperm-Ovum Interactions|*DE
MeSH Heading: Drug Synergism; Female; Human; Lactate Dehydrogenase|ME; Male; Nitroblue Tetrazolium|ME; Oxidation-Reduction; Spermatozoa|DE/ME; Support, U.S. Gov't, P.H.S.

Publication Type: JOURNAL ARTICLE
ISSN: 0010-7824
Country of Publication: UNITED STATES

Record 44 from database: MEDLINE
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Title: End-stage renal failure due to analgesic nephropathy, its changing pattern and cardiovascular mortality. EDTA-ERA Registry Committee.
Author: Brunner FP; Selwood NH
Address: Department fÂur Innere Medizin, UniversitÂat Basel, Switzerland.
Source: Nephrol Dial Transplant, 1994, 9:10, 1371-6
Abstract: The changing pattern of prevalence and age distribution of analgesic nephropathy as a cause of end-stage renal failure (ESRF) in patients on RRT was analysed using the EDTA-ERA Registry's files. Comparing 1990 to 1981, the percentage of patients with analgesic nephropathy decreased in many European countries and the Registry's average came down from 3 to 2%. The highest prevalence was noted for Switzerland, which showed a decrease from 28 in 1981 to 12% in 1990. During the same interval the age distribution shifted to the right with an increase in median age from 57 to 63 at start of RRT for analgesic nephropathy. In Switzerland the age-specific acceptance rate to RRT for patients with analgesic nephropathy decreased to less than 1/3 in the age cohorts below 55 but increased in those aged 65 or older. This increase in the elderly cohorts appeared to be related to the growing acceptance rate to RRT of elderly patients in general rather than to an increasing incidence of ESRF due to analgesic nephropathy. Mortality in general and death rates due to cardiovascular causes were found not to differ in RRT patients with analgesic nephropathy from that of other standard primary renal diseases (excluding diabetic nephropathy and systemic diseases). Some 20 years after withdrawal of phenacetin from the analgesic market, analgesic nephropathy all but disappeared as a cause of ESRF in Sweden and Denmark, and the same may be expected to occur in countries like Switzerland, Belgium, and others in the not too far distant future.
Language of Publication: English
Unique Identifier: 95115920

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MeSH Heading (Major): Cardiovascular Diseases|CO/*MO; Kidney Failure, Chronic|*CI/CO/*EP/TH; Phenacetin|*AE/TU; Renal Replacement Therapy|*
MeSH Heading: Age Distribution; Aged; Europe|EP; Female; Human; Incidence; Male; Middle Age; Pharmacoepidemiology|TD; Prevalence; Registries; Sex Distribution; Support, Non-U.S. Gov't

Publication Type: JOURNAL ARTICLE
ISSN: 0931-0509
Country of Publication: ENGLAND

Record 45 from database: MEDLINE
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Title: Comparison of heparin and EDTA transport tubes for detection of cytomegalovirus in leukocytes by shell vial assay, pp65 antigenemia assay, and PCR.
Author: Storch GA; Gaudreault Keener M; Welby PC
Address: Edward Mallinckrodt Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri 63110.
Source: J Clin Microbiol, 1994 Oct, 32:10, 2581-3
Abstract: The anticoagulants heparin and EDTA were compared for inhibitory effects on the detection of cytomegalovirus from washed leukocytes in specimen transport tubes. Evaluation was made by the centrifugation/shell vial culture technique, the pp65 antigenemia assay, and PCR. For each assay, the results with heparin and EDTA were equivalent.
Language of Publication: English
Unique Identifier: 95113979

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MeSH Heading (Major): Antigens, Viral|*BL; Cytomegalovirus|DE/*IP; Edetic Acid|*PD; Heparin|*PD; Leukocytes|*VI; Phosphoproteins|*BL; Polymerase Chain Reaction|*; Viral Matrix Proteins|*BL
MeSH Heading: Blood Specimen Collection; Comparative Study; Human; Support, Non-U.S. Gov't

Publication Type: JOURNAL ARTICLE
ISSN: 0095-1137
Country of Publication: UNITED STATES

Record 46 from database: MEDLINE
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Title: A new type of pseudothrombocytopenia: EDTA-mediated agglutination of platelets bearing Fab fragments of a chimaeric antibody.
Author: Christopoulos CG; Machin SJ
Address: Department of Haematology, University College London.
Source: Br J Haematol, 1994 Jul, 87:3, 650-2
Abstract: In vitro agglutination of platelets leading to low automated platelet counts was observed in EDTA-anticoagulated blood from human volunteers receiving infusions of Fab fragments of a chimaeric monoclonal antibody to platelet glycoprotein IIb-IIIa. This pseudothrombocytopenia depended on the presence of chimaeric Fab on the platelet surface and was not seen when sodium citrate was used as anticoagulent. Preliminary evidence suggests that this phenomenon might be mediated by immunoglobulin G reactive with the human component of the chimaeric Fab. It is important to exclude pseudothrombocytopenia when low automated platelet counts are reported in association with the administration of chimaeric anti-platelet antibodies.
Language of Publication: English
Unique Identifier: 95085973

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MeSH Heading (Major): Blood Platelets|*IM; Edetic Acid|*PD; Platelet Membrane Glycoproteins|*IM; Thrombocytopenia|*BL/DI/IM
MeSH Heading: Chimeric Proteins|IM; Flow Cytometry; Hemagglutination|DE; Human; IgG|IM; Immunoglobulins, Fab|IM; Platelet Count

Publication Type: JOURNAL ARTICLE
ISSN: 0007-1048
Country of Publication: ENGLAND

Record 47 from database: MEDLINE
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Title: EDTA-plasma vs serum differences in cholesterol, high-density-lipoprotein cholesterol, and triglyceride as measured by several methods.
Author: Beheshti I; Wessels LM; Eckfeldt JH
Address: Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis 55455-0392.
Source: Clin Chem, 1994 Nov, 40:11 Pt 1, 2088-92
Abstract: To investigate EDTA-plasma/serum (P/S) differences, we collected paired samples from 25 volunteers and measured total cholesterol (TC), triglyceride (TG) and high-density-lipoprotein cholesterol (HDLC), using the Cobas FARA, Ektachem 700, DuPont Dimension, and Baxter Paramax Analyzers. The mean (SD) P/S ratios for TC, HDLC, and TG concentrations were, respectively: 0.980 (0.0171), 1.063 (0.0704), and 0.961 (0.363) for Paramax; 0.976 (0.0189), 1.034 (0.1091), and 0.950 (0.557) for Dimension; 1.003 (0.0221), 1.059 (0.0304), and 0.988 (0.0179) for Ektachem; and 0.993 (0.0162), 1.063 (0.0830), and 1.013 (0.0410) for Cobas. We conclude that P/S ratios vary by analytical methods, and that HDLC ratios tend to be larger in magnitude and in the opposite direction from TC and TG. Both effects lead to significant biases in computed disease risk.
Language of Publication: English
Unique Identifier: 95043391

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MeSH Heading (Major): Chemistry, Clinical|MT/SN/*ST; Cholesterol|*BL; Edetic Acid|*; Lipoproteins, HDL Cholesterol|*BL; Plasma|*; Triglycerides|*BL
MeSH Heading: Adult; Bias (Epidemiology); Female; Human; Male; Middle Age; Reference Values

Publication Type: JOURNAL ARTICLE
ISSN: 0009-9147
Country of Publication: UNITED STATES

Record 48 from database: MEDLINE
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Title: Random locomotion and chemotaxis of human blood polymorphonuclear leukocytes (PMN) in the presence of EDTA: PMN in close quarters require neither leukocyte integrins nor external divalent cations.
Author: Malawista SE; de Boisfleury Chevance A
Address: Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06510, USA. stephen.malawista@yale.edu
Source: Proc Natl Acad Sci U S A, 1997 Oct, 94:21, 11577-82
Abstract: Divalent cations are thought essential for motile function of leukocytes in general, and for the function of critical adhesion molecules in particular. In the current study, under direct microscopic observation with concomitant time-lapse video recording, we examined the effects of 10 mM EDTA on locomotion of human blood polymorphonuclear leukocytes (PMN). In very thin slide preparations, EDTA did not impair either random locomotion or chemotaxis; motile behavior appeared to benefit from the close approximation of slide and coverslip ("chimneying"). In preparations twice as thick, PMN in EDTA first exhibited active deformability with little or no displacement, then rounded up and became motionless. However, on creation of a chemotactic gradient, the same cells were able to orient and make their way to the target, often, however, losing momentarily their purchase on the substrate. In either of these preparations without EDTA, specific antibodies to beta2 integrins did not prevent random locomotion or chemotaxis, even when we added antibodies to beta1 and alphavbeta3 integrins and to integrin-associated protein, and none of these antibodies added anything to the effects of EDTA. In the more turbulent environment of even more media, effects of anti-beta2 integrins became evident: PMN still could locomote but adhered to substrate largely by their uropods and by uropod-associated filaments. We relate these findings to the reported independence from integrins of PMN in certain experimental and disease states. Moreover, we suggest that PMN locomotion in close quarters is not only integrin-independent, but independent of external divalent cations as well.
Language of Publication: English
Unique Identifier: 97471009

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MeSH Heading (Major): Calcium|*PD; Chemotaxis, Leukocyte|*DE/PH; Edetic Acid|*PD; Integrins|IM/*PH; Magnesium|*PD; Neutrophils|DE/*PH
MeSH Heading: Antibodies, Monoclonal|PD; Antigens, CD18|IM/PH; Antigens, CD29|IM/PH; Cations, Divalent|PD; Cell Movement|DE/PH; Human; In Vitro; Receptors, Vitronectin|IM/PH; Support, Non-U.S. Gov't; Support, U.S. Gov't, P.H.S.

Publication Type: JOURNAL ARTICLE
ISSN: 0027-8424
Country of Publication: UNITED STATES

Record 49 from database: MEDLINE
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Title: Long-term precision of glomerular filtration rate measurements using 51Cr-EDTA plasma clearance.
Author: Blake GM; Roe D; Lazarus CR
Address: Department of Nuclear Medicine, Guy's Hospital, London, UK.
Source: Nucl Med Commun, 1997 Aug, 18:8, 776-84
Abstract: The long-term precision of chromium-51 ethylenediamine tetraacetic acid (51Cr-EDTA) measurements of glomerular filtration rate (GFR) was evaluated in a retrospective study of data obtained over a 12 year period. Each GFR measurement was derived from plasma samples taken at 2, 3 and 4 h following injection of 3 MBq 51Cr-EDTA. The records of 7507 patients were reviewed, from which 55 subjects were identified as having had studies on 10 or more occasions. The mean number of studies per patient was 12.9 (range 10-23) over a mean period of 9.4 years (range 4.3-11.8 years). Plots of GFR, clearance half-life, (T1/2) and volume of distribution (VD) were drawn for each patient and used to identify subjects showing linear changes with time that could be fitted using linear regression. Each residual was expressed as a percentage of the expected value calculated from the regression line and all the residuals combined to give histograms for GFR, T1/2 and VD. Each histogram was fitted with a normal distribution between the -3 S.D. and +3 S.D. limits using weighted least squares. Final results for the coefficient of variation were: GFR 9.8%, T1/2 6.7%, VD 9.4%. The precision errors were used to calculate the smallest statistically significant change measurable by the 51Cr-EDTA technique. With 10% significance and 80% power, the smallest measureable change was 30% for GFR and 20% for T1/2. Unless there are clinical grounds for thinking that a patient's volume of distribution has changed, T1/2 is the optimal parameter for identifying real changes in renal function.
Language of Publication: English
Unique Identifier: 97439057

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MeSH Heading (Major): Chromium Radioisotopes|BL/*DU/*PK; Glomerular Filtration Rate|*; Kidney Diseases|PP/*RI
MeSH Heading: Edetic Acid; Half-Life; Human; Least-Squares Analysis; Normal Distribution; Regression Analysis; Reproducibility of Results; Retrospective Studies; Sensitivity and Specificity

Publication Type: JOURNAL ARTICLE
ISSN: 0143-3636
Country of Publication: ENGLAND

Record 50 from database: MEDLINE
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Title: Technetium (99mTc)-labelled white cell scanning, 51Cr-EDTA and 14C-mannitol-labelled intestinal permeability studies: non-invasive methods of diagnosing acute intestinal graft-versus-host disease [see comments]
Author: Mahendra P; Bedlow AJ; Ager S; Ancliff PJ; Wraight EP; Marcus RE
Address: Department of Haematology, Addenbrookes NHS Trust, Cambridge, UK.
Source: Bone Marrow Transplant, 1994 Jun, 13:6, 835-7
Abstract: We describe a case of a 38-year-old female who presented with diarrhoea and abdominal pain 27 days after a second 'top-up' allogeneic marrow infusion for acute myeloid leukaemia (AML) in first remission. A clinical diagnosis of gut graft-versus-host disease (GVHD) was made. Technetium (99mTc)-labelled white cell scanning and intestinal permeability studies using 51Cr-EDTA and 14C-mannitol were undertaken to confirm the diagnosis. The 99mTc white cell scan showed extensive uptake in the small bowel and the urinary excretion of 51Cr-EDTA was increased, the results being consistent with intestinal inflammation and gut GVHD. 99mTc white cell scanning and intestinal permeability studies may assist in the diagnosis of gut GVHD and in assessing its extent and response to treatment.
Language of Publication: English
Unique Identifier: 95004114

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MeSH Heading (Major): Edetic Acid|*PK; Graft vs Host Disease|*DI/ET/PP; Intestinal Absorption|*PH; Intestinal Diseases|*DI/ET/PP; Leukocyte Count|*; Mannitol|*PK
MeSH Heading: Acute Disease; Adult; Bone Marrow Transplantation|AE; Carbon Radioisotopes; Case Report; Chromium Radioisotopes; Female; Human; Intestines|PH; Leukemia, Myeloid|TH; Methods; Technetium Compounds

Publication Type: JOURNAL ARTICLE
ISSN: 0268-3369
Country of Publication: ENGLAND

Record 51 from database: MEDLINE
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Title: EDTA dependent pseudothrombocytopenia caused by antibodies against the cytoadhesive receptor of platelet gpIIB-IIIA.
Author: Casonato A; Bertomoro A; Pontara E; Dannhauser D; Lazzaro AR; Girolami A
Address: University of Padua Medical School, Institute of Medical Semeiotics, Italy.
Source: J Clin Pathol, 1994 Jul, 47:7, 625-30
Abstract: AIMS--To clarify the mechanisms involved in the development of EDTA dependent pseudothrombocytopenia, particularly the platelet receptors. METHODS--Platelets were measured in 33 patients with pseudothrombocytopenia, using different anticoagulants to collect blood samples (direct test). The results were compared with the counts obtained by adding patients' serum or immunoglobulins to normal blood samples (indirect test). The role of platelet function was explored using ASA, PGE1, and apyrase as platelet inhibitors. The contribution of platelet receptor/s was investigated using antigens to gpIb-IX and gpIIb-IIIa monoclonal antibodies. Immunoglobulin class was estimated by the ability of IgG, IgA, and IgM antibodies to prevent platelet clumping. RESULTS--Agglutinating antibodies were IgA in 40%, IgG in 30%, and IgM in 10% of patients studied. Both patients' serum and immunoglobulins induced platelet clumping in normal samples anticoagulated with EDTA (indirect test). This was prevented by incubation of blood samples at 37 degrees C and almost completely inhibited by the platelet inhibitors ASA, PGE1, and apyrase. Pseudothrombocytopenia was also entirely prevented by an antigen to gpIIb-IIIa monoclonal antibody that recognises fibrinogen and the von Willebrand factor binding site. Pseudothrombocytopenia was almost completely abolished after the addition of RGD peptide, the recognition sequence of cytoadhesive proteins. CONCLUSIONS--These findings suggest that EDTA dependent pseudothrombocytopenia is caused by agglutinating antibodies that recognise cytoadhesive receptors on platelet gpIIb-IIIa and that an efficient platelet metabolism is required.
Language of Publication: English
Unique Identifier: 94375569

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MeSH Heading (Major): Antibodies, Monoclonal|*IM; Edetic Acid|*; Platelet Membrane Glycoproteins|*IM; Thrombocytopenia|*IM
MeSH Heading: Adult; Comparative Study; Female; Human; IgA|AN; IgG|AN; IgM|AN; Male; Oligopeptides|IM; Platelet Function Tests; Support, Non-U.S. Gov't

Publication Type: JOURNAL ARTICLE
ISSN: 0021-9746
Country of Publication: ENGLAND

Record 52 from database: MEDLINE
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Title: Dynamic positron emission tomography for study of cerebral hemodynamics in a cross section of the head using positron-emitting 68Ga-EDTA and 77Kr.
Author: Yamamoto YL; Thompson CJ; Meyer E; Robertson JS; Feindel W
Address
Source: J Comput Assist Tomogr, 1977 Jan, 1:1, 43-56
Abstract: Dynamic positron emission tomographic studies were performed on over 120 patients with occlusive cerebrovascular disease, arteriovenous malformations, and brain tumors, using the positron section scanner, consisting of a ring of 32 scintillation detectors. The radiopharmaceuticals were nondiffusible 68Ga-EDTA for transit time and uptake studies and the diffusible tracer, 77Kr, for quantitative regional cerebral blood flow studies in every square centimeter of the cross section of the head. The results of dynamic positron emission tomography in correlation with the results from the gamma scintillation camera dynamic studies and computed tomography (CT) scans are discussed.
Language of Publication: English
Unique Identifier: 78242680

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MeSH Heading (Major): Brain Neoplasms|*RA; Cerebrovascular Circulation|*; Cerebrovascular Disorders|*RA; Gallium Radioisotopes|*DU; Krypton|*DU; Radioisotopes|*DU; Tomography, X-Ray|*
MeSH Heading: Adult; Case Report; Female; Human; Male; Middle Age

Publication Type: JOURNAL ARTICLE
ISSN: 0363-8715
Country of Publication: UNITED STATES

Record 53 from database: MEDLINE
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Title: Sequential preparation of highly purified microvillous and basal syncytiotrophoblast membranes in substantial yield from a single term human placenta: inhibition of microvillous alkaline phosphatase activity by EDTA.
Author: Eaton BM; Oakey MP
Address: Academic Department of Obstetrics and Gynaecology, Charing Cross Medical School, Chelsea Hospital, London, UK.
Source: Biochim Biophys Acta, 1994 Jul, 1193:1, 85-92
Abstract: The human placental syncytiotrophoblast is a highly polarised epithelial layer responsible for regulating materno-fetal exchange. We here describe a novel procedure for isolating paired fractions of the maternal-facing and fetal-facing plasma membranes from this syncytium, from a single placenta, without the need for homogenisation procedures. This reduces the potential for contamination of these membrane fractions by intracellular membranes, or from plasma membranes from other cell types within the placenta. Microvillous membrane vesicles (MVM) were obtained by gentle stirring of dispersed villous tissue. The tissue sedimented at the end of this procedure was subjected to sequential ultrasonication to release the basal membrane (BM). Crude MVM was subsequently purified on a discontinuous sucrose gradient. Crude BM was further purified using either discontinuous Ficoll or sucrose gradients. The Ficoll procedure, while producing a BM fraction extremely enriched in marker enzyme, resulted in unacceptably low protein recoveries and hence the sucrose gradient procedure was also adopted for BM. Yields for MVM and BM produced on sucrose density gradients approached 30 mg/100 g tissue. The MVM fraction was composed of vesicles of 232 +/- 9 (S.E.) nm diameter of which nearly 90% were 'right side out'. These membranes were 37-fold enriched in the marker enzyme alkaline phosphatase. Purified BM vesicles were 317 +/- 14 nm in diameter, also approximately 90% 'right side out' and over 40-fold enriched in dihydroalprenolol binding. Cross-contamination or contamination from intracellular membranes was negligible. MVM alkaline phosphatase activity was shown to be inhibitable in a dose- and time-dependent manner by EDTA present in the storage buffer.
Language of Publication: English
Unique Identifier: 94312424

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MeSH Heading (Major): Alkaline Phosphatase|*AI; Edetic Acid|*PD; Giant Cells|*EN/UL; Placenta|*EN/UL; Trophoblast|*EN/UL
MeSH Heading: Cell Fractionation; Cell Membrane|EN; Human; Microvilli|EN

Publication Type: JOURNAL ARTICLE
ISSN: 0006-3002
Country of Publication: NETHERLANDS

Record 54 from database: MEDLINE
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Title: Simultaneous urography and determination of glomerular filtration rate. A comparison of total plasma clearances of iohexol and 51Cr-EDTA in plegic patients.
Author: Lundqvist S; Hietala SO; Berglund C; Karp K
Address: Department of Diagnostic Radiology, University Hospital, UmeÁa, Sweden.
Source: Acta Radiol, 1994 Jul, 35:4, 391-5
Abstract: The total plasma clearance of iohexol at urography and 51Cr-EDTA was compared in 31 patients with di- or tetraparesis. A reference 51Cr-EDTA clearance was also performed 24 hours prior to the urography. The GFR was calculated from one, 2 or 4 plasma samples collected 180, 210, 240 and 270 min after the injection. An X-ray fluorescence analyzer was used for the analysis of iohexol in plasma as well as the contrast medium clearance calculations. It was shown that single or multiple sample clearance of iohexol and 51Cr-EDTA were equivalent methods for measurement of the GFR. The GFR was not affected by iohexol in a dose routinely used for urography. It was concluded that the patient comfort is improved if 51Cr-EDTA clearance is replaced by contrast medium clearance in association with urography.
Language of Publication: English
Unique Identifier: 94280927

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MeSH Heading (Major): Chromium Radioisotopes|*BL; Edetic Acid|PD/*PK; Glomerular Filtration Rate|DE/*PH; Iohexol|PD/*PK; Paraplegia|BL/PP/*RA; Quadriplegia|BL/PP/*RA; Urography|*
MeSH Heading: Adult; Aged; Body Surface Area; Comparative Study; Female; Fluorescence; Human; Male; Metabolic Clearance Rate; Middle Age; Time Factors

Publication Type: JOURNAL ARTICLE
ISSN: 0284-1851
Country of Publication: DENMARK

Record 55 from database: MEDLINE
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Title: Oxidation of human insulin-like growth factor I in formulation studies. 3. Factorial experiments of the effects of ferric ions, EDTA, and visible light on methionine oxidation and covalent aggregation in aqueous solution.
Author: Fransson JR
Address: Department of Pharmaceutical Technology, Pharmacia & Upjohn, Stockholm, Sweden.
Source: J Pharm Sci, 1997 Sep, 86:9, 1046-50
Abstract: The influence of ferric ions, EDTA, and visible light on the oxidation of methionine and the covalent reducible and nonreducible dimerization in human Insulin-like Growth Factor I (hIGF-I) in aqueous (1 mM) phosphate buffer solution were studied. A reduced factorial experiment with two levels of each factor was used. Regression models for the three responses were constructed with partial least square (PLS) analysis. The hIGF-I variants were quantified by reversed-phase high-performance liquid chromatography (RP-HPLC), gel filtration, and reduced sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The formation of the hIGF-I variants in aqueous solution at pH 6.1 exhibited different dependencies on the variables studied. The oxidation of methionine was affected mainly by visible light and the combination of 10 ppm ferric ions and 20 ppm EDTA, whereas ferric ions alone had no significant effect. The covalent dimerization of hIGF-I was correlated to visible light and ferric ions. The interaction effects of ferric ions with either visible light or EDTA were also significant on the dimerization rates. Both reducible and nonreducible soluble covalent dimers were formed, with the reducible dimer being the most prominent. The oxidation of methionine 59 in hIGF-I is catalyzed by light and by ferric ions in combination with EDTA. The covalent dimerization of hIGF-I is mainly affected by light and by ferric ions. Both reducible and nonreducible dimerization increased by oxidative conditions. Human IGF-I appears to dimerize covalently by both disulfide scrambling and by a radical-promoted nondisulfide pathway. EDTA is necessary for ferric ions to be active in the oxidation of methionine in hIGF-I but not for the covalent dimerization.
Language of Publication: English
Unique Identifier: 97440602

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MeSH Heading (Major): Insulin-Like Growth Factor I|*CH/RE; Methionine|*CH/RE
MeSH Heading: Amino Acid Sequence; Drug Packaging; Edetic Acid|CH; Ferric Compounds|CH; Human; Light; Molecular Sequence Data; Oxidation-Reduction; Regression Analysis; Solutions

Publication Type: JOURNAL ARTICLE
ISSN: 0022-3549
Country of Publication: UNITED STATES

Record 56 from database: MEDLINE
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Title: In vivo inhibition of serum dopamine-beta-hydroxylase by CaNa2 EDTA injection.
Author: De Paris P; Caroldi S
Address: Istituto di Medicina del Lavoro, University of Padova, Italy.
Source: Hum Exp Toxicol, 1994 Apr, 13:4, 253-6
Abstract: 1. In humans CaNa2EDTA increases urinary excretion of lead as well as that of essential metals such as zinc, ferrum and manganese but not that of copper. 2. We studied the effect of CaNa2EDTA injection on serum dopamine-beta-hydroxylase, a copper-dependent enzyme, in three male lead welders hospitalized for suspected lead poisoning. 3. Injection of CaNa2EDTA (1000 mg i.v.) resulted in rapid reduction of serum dopamine-beta-hydroxylase activity which returned to normal level within 24 hours. 4. Copper sulphate (8 microns) but not ethylmaleimide (10 mM) restored in vitro serum dopamine-beta-hydroxylase activity to pre-dosing values which indicates that CaNa2EDTA inhibits dopamine-beta-hydroxylase by removing copper from the enzyme. 5. An inverse dose effect relationship between injected CaNa2EDTA and residual serum dopamine-beta-hydroxylase activity was observed. 6. In spite of the reported lack of changes in copper serum concentration, CaNa2EDTA significantly affects the activity of a copper-dependent enzyme in the serum. 7. As CaNa2EDTA is currently the drug of choice for the treatment of asymptomatic lead poisoning in children, the effect of CaNa2EDTA on copper-enzymes requires further investigation.
Language of Publication: English
Unique Identifier: 94263619

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MeSH Heading (Major): Dopamine beta-Hydroxylase|*AI/*BL; Edetic Acid|*PD
MeSH Heading: Adult; Copper|ME; Human; Lead Poisoning|ME; Male

Publication Type: JOURNAL ARTICLE
ISSN: 0960-3271
Country of Publication: ENGLAND

Record 57 from database: MEDLINE
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Title: Effect of different concentrations of EDTA on smear removal and collagen exposure in periodontitis-affected root surfaces.
Author: Blomlöf J; Blomlöf L; Lindskog S
Address: Department of Oral Histology and Cell Biology, School of Dentistry, Karolinska Institutet, Stockholm, Sweden.
Source: J Clin Periodontol, 1997 Aug, 24:8, 534-7
Abstract: The purpose of the present study was to explore the possibility of obtaining an acceptable smear-removing and collagen-exposing effect following EDTA etching with concentrations lower than supersaturation (24%). A flat dentin surface was created on human teeth extracted due to severe periodontitis. The teeth were etched with the following concentrations of EDTA for 2 min: 1.5% (group I), 5% (group II), 15% (group III) and 24% (group IV), and evaluated with scanning electron microscopy with respect to smear layer removal and exposure of root-associated collagen fibers. It was found that neither 1.5% nor 5% EDTA etching was sufficient to dissolve the smear layer to any higher degree. Etching with 15% EDTA dissolved the smear more effectively than the lower concentrations and collagen fibers were sparsely found. Following etching with 24% EDTA (group IV), significantly less smear was seen on the dentin surfaces than in any of the other groups and collagen fibers were seen covering the entire intertubular dentin surfaces. Based on these findings, the concentration of EDTA should be somewhere between 15 to 24% in order to obtain an acceptable smear removing and collagen-exposing effect within a clinically acceptable time period.
Language of Publication: English
Unique Identifier: 97411361

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MeSH Heading (Major): Chelating Agents|AD/*PD; Collagen|*DE/UL; Dentin|*DE/UL; Edetic Acid|AD/*PD; Periodontitis|*PA/PP; Smear Layer|*; Tooth Root|*DE/UL
MeSH Heading: Acid Etching, Dental; Evaluation Studies; Human; Microscopy, Electron, Scanning

Publication Type: JOURNAL ARTICLE
ISSN: 0303-6979
Country of Publication: DENMARK

Record 58 from database: MEDLINE
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Title: Inhibition of paraoxonase activity in human liver microsomes by exposure to EDTA, metals and mercurials.
Author: Gonzalvo MC; Gil F; Hernández AF; Villanueva E; Pla A
Address: Department of Legal Medicine, Faculty of Medicine, Granada, Spain.
Source: Chem Biol Interact, 1997 Aug, 105:3, 169-79
Abstract: Inhibition of paraoxon hydrolase (paraoxonase) activity by 'in vitro' exposure to EDTA, Mg2+, Co2+, Ba2+, La3+, Zn2+, Cu2+, Hg2+, p-hydroxymercuribenzoate (p-OH-MB) and phenyl mercuric acetate (PMA) was investigated in human liver microsomes. Enzyme activity was totally inhibited by 1 mM EDTA in a time-dependent manner, in contrast to previous data obtained in rat liver where an EDTA-resistant fraction was detected. The possible influence of postmortem changes in these results was checked in a parallel experiment using rat livers with different postmortem intervals. From our results the existence in human liver of an EDTA-resistant fraction cannot be discarded. Ba, La and PMA showed immediate inhibition. By contrast the other compounds tested were time-dependent inhibitors. Ba and Zn showed the highest IC50 values. Cu and mercurials (Hg, p-OH-MB, PMA) were the most potent inhibitors of human liver paraoxonase. Kinetic analysis (Lineweaver-Burk and Dixon plots) indicated that different inhibitors exhibit different inhibition patterns: competitive (EDTA, Ba, La, Cu, p-OH-MB and PMA), non competitive (Zn) and mixed (Hg). The pretreatment of sample with dithiothreitol (DTT) protects against the inhibitory effect of mercurials. Furthermore after inhibition by mercurials the activity was restored by DTT. These results confirmed the essential role of the -SH groups to maintain the catalytic activity of paraoxonase and suggest the existence of two types of -SH groups that could differ in their localization.
Language of Publication: English
Unique Identifier: 97437462

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MeSH Heading (Major): Edetic Acid|*PD; Enzyme Inhibitors|*PD; Esterases|*AI/ME; Mercury Compounds|*PD; Metals|*PD; Microsomes, Liver|DE/*EN
MeSH Heading: Animal; Dithiothreitol|PD; Enzyme Activation; Human; Kinetics; Rats; Sulfhydryl Compounds|ME; Support, Non-U.S. Gov't

Publication Type: JOURNAL ARTICLE
ISSN: 0009-2797
Country of Publication: IRELAND

Record 59 from database: MEDLINE
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Title: 90Y-labeled antibody uptake by human tumor xenografts and the effect of systemic administration of EDTA.
Author: Rowlinson Busza G; Snook D; Epenetos AA
Address: Tumour Targeting Laboratory, Imperial Cancer Research Fund Oncology Unit, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
Source: Int J Radiat Oncol Biol Phys, 1994 Mar, 28:5, 1257-65
Abstract: PURPOSE: A human tumor xenograft model was used to compare the tumor and normal tissue uptake of a tumor-associated monoclonal antibody radiolabeled with 125I or 90Y. METHODS AND MATERIALS: Nude mice bearing SC xenografts of the human colon adenocarcinoma, HT29, were injected with a mixture of 125I- and 90Y-DTPA-labeled AUA1 monoclonal antibody, which recognizes an antigen expressed on the surface of the tumor cells. In addition, the effect of systemic ethylenediaminetetraacetic acid (EDTA) administration on 90Y-labeled antibody clearance, tumor uptake of antibody and bone accumulation of 90Y was studied in a nude mouse model of intraperitoneal cancer. RESULTS: Both the absolute amount (%id.g-1) and the tumor:normal tissue ratios were superior for the 90Y-labeled antibody, compared with the iodinated antibody, with the notable exception of bone. These results suggest that 90Y is a preferable isotope to iodine for radioimmunotherapy of solid masses, but that myelotoxicity, due to bone uptake of released 90Y, will limit the radiation dose which can be given when DTPA is used to chelate the 90Y. The 90Y-labeled antibody showed similar serum stability in vitro in the presence or absence of EDTA after incubation for up to 48 h. In vivo, urine excretion of 90Y was significantly enhanced in mice receiving daily injections of 20 mg EDTA for 3 days, commencing 2 h after intraperitoneal antibody administration, compared with control mice. There was no significant difference in the tumor uptake of 90Y-labeled antibody in EDTA-treated and control mice at any time-point up to 9 days postinjection. However, the bone levels of 90Y were significantly reduced in EDTA-treated mice at all times from 1 to 9 days. CONCLUSION: Based on these results, it should be possible to increase the amount of 90Y-labeled antibody administered, by chelating the released 90Y with systemic EDTA to facilitate its excretion, without compromising tumor uptake of radiolabeled antibody.
Language of Publication: English
Unique Identifier: 94230046

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MeSH Heading (Major): Antibodies, Monoclonal|*ME; Edetic Acid|*PD; Neoplasms, Experimental|*ME; Yttrium Radioisotopes|*DU
MeSH Heading: Animal; Colonic Neoplasms|ME; Female; Human; Iodine Radioisotopes|DU; Mice; Mice, Inbred BALB C; Mice, Nude; Neoplasm Transplantation; Tissue Distribution; Transplantation, Heterologous

Publication Type: JOURNAL ARTICLE
ISSN: 0360-3016
Country of Publication: UNITED STATES

Record 60 from database: MEDLINE
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Title: Quantitative capillary electrophoresis/ion spray tandem mass spectrometry determination of EDTA in human plasma and urine.
Author: Sheppard RL; Henion J
Address: Cornell University, Ithaca, New York 14850, USA.
Source: Anal Chem, 1997 Aug, 69:15, 2901-7
Abstract: A quantitative method has been developed for the determination of EDTA in human plasma and urine. The samples are prepared with automated anion-exchange solid-phase extraction using 100 microL of human plasma. The extracts are analyzed by capillary electrophoresis/mass spectrometry using selected reaction monitoring in the negative ion mode. Large-volume injections (10% of the CE capillary volume) are used to improve the concentration level of detection via field-amplified sample injection. The first reported validation of a CE/MS/MS technique was carried out for this method. Using a 13C stable-label isotope for the internal standard, the lower level of detection and lower level of quantitation were determined to be 7.3 and 14.6 ng/mL EDTA in human plasma, respectively. The injection precision had a relative standard deviation (RSD) of 6.1%. The intra-assay precision was less than 15% RSD. The intra-assay accuracy was less than +/- 12% bias from the nominal concentration. The interassay precision was less than 18% RSD and the interassay accuracy was less than +/- 9% bias from the nominal concentration.
Language of Publication: English
Unique Identifier: 97397182

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MeSH Heading (Major): Blood Chemical Analysis|*; Edetic Acid|*AN; Electrophoresis, Capillary|*MT; Spectrum Analysis, Mass|*MT; Urinalysis|*
MeSH Heading: Human; Reproducibility of Results; Sensitivity and Specificity; Support, Non-U.S. Gov't

Publication Type: JOURNAL ARTICLE
ISSN: 0003-2700
Country of Publication: UNITED STATES

Record 61 from database: MEDLINE
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Title: EDTA and the absorption of iron from food.
Author: MacPhail AP; Patel RC; Bothwell TH; Lamparelli RD
Address: Department of Medicine, University of the Witwatersrand, Johannesburg, South Africa.
Source: Am J Clin Nutr, 1994 Mar, 59:3, 644-8
Abstract: Iron EDTA is an effective fortificant in meals of low iron bioavailability. Na2EDTA, added to food to prevent oxidation, enhances iron bioavailability by chelating added iron. This study examines the optimal ratio of EDTA to iron causing enhanced iron absorption. Iron absorption from a rice-based meal of low iron bioavailability containing increasing molar ratios of EDTA to iron, was compared in 127 women volunteers by using standard double isotope techniques. Iron deficiency was present in 38% of the women. Mean standardized absorptions, at EDTA-iron ratios of 0.25, 0.5, and 1, were 11.3%, 13.5%, and 8.8%, respectively, compared with 3.8% when no Na2EDTA was present. In meals of high iron bioavailability, Na2EDTA (EDTA:Fe, 1.0) produced little enhancement (potato-based meal) nor inhibited iron absorption (apple-based meal). Na2EDTA added to meals with molar ratios of EDTA to iron between 1.0 and 0.25 significantly increases iron absorption provided the meal is of low iron bioavailability.
Language of Publication: English
Unique Identifier: 94160914

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MeSH Heading (Major): Edetic Acid|*ME/*PD; Ferrous Compounds|*ME; Intestinal Absorption|DE/*PH; Iron|*ME
MeSH Heading: Diet; Female; Ferritin|BL; Hemoglobins|ME; Human; Iron Radioisotopes; Radioisotope Dilution Technique; Support, Non-U.S. Gov't; Transferrin|ME

Publication Type: JOURNAL ARTICLE
ISSN: 0002-9165
Country of Publication: UNITED STATES

Record 62 from database: MEDLINE
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Title: Back pain following epidural anesthesia with 2-chloroprocaine (EDTA-free) or lidocaine [see comments]
Author: Drolet P; Veillette Y
Address: Department of Anesthesia, Maisonneuve-Rosemont Hospital, University of Montreal, Quebec.
Source: Reg Anesth, 1997 Jul, 22:4, 303-7
Abstract: BACKGROUND AND OBJECTIVES: Severe lumbar pain following epidural injection of 2-chloroprocaine is usually associated with the Nesacaine-MPF solution available in the United States. The purpose of this study was to determine if the solution distributed in Canada (Nesacaine-CE), which contains calcium disodium edetate (0.1 mg/mL) and sodium bisulfite (0.7 mg/mL) but no disodium ethylenediaminetetraacetic acid, is associated with back pain or spasm when compared with epidural lidocaine. METHODS: With use of a prospective, double-blind, randomized design, 30 patients scheduled to undergo outpatient knee arthroscopy under epidural anesthesia were divided into two groups to received 30 mL of either Nesacaine-CE 3% (group A) or lidocaine 1.33% (group B). Postoperative pain in the lumbar area was assessed twice by a 10-cm visual analog scale (VAS) before patients left the hospital and 24 hours later by phone. The lumbar area was palpated to search for muscle spasm before discharge from hospital. RESULTS: More patients receiving Nesacaine-CE than receiving lidocaine suffered from back pain in the recovery room (four vs none P = .03) and before leaving the hospital (nine vs one P = .001). Higher VAS scores (mean +/- SE) were obtained after Nesacaine CE then after lidocaine in the recovery room (0.5 +/- 0.24 vs 0.0 +/- 0.0, p = .049) and before leaving the hospital (1.8 +/- 0.5 vs 0.1 +/- 0.1, P = .001). No difference existed 24 hours later between the two groups with regard to the prevalence of back pain or VAS scores. No muscle spasm was detected. CONCLUSION: No cases of severe backache were observed. However, epidural Nesacaine-CE 3% was associated with mild back pain, generally confined to the area of needle insertion, when compared with lidocaine 1.33%.
Language of Publication: English
Unique Identifier: 97366399

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MeSH Heading (Major): Anesthesia, Epidural|*AE; Anesthetics, Local|*AE; Back Pain|*ET; Lidocaine|*AE; Procaine|*AA/AE
MeSH Heading: Adult; Double-Blind Method; Female; Human; Male; Prospective Studies

Publication Type: CLINICAL TRIAL; JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
ISSN: 0146-521X
Country of Publication: UNITED STATES

Record 63 from database: MEDLINE
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Title: Clinical evaluation of the efficacy of EDTA solution as an endodontic irrigant.
Author: Yoshida T; Shibata T; Shinohara T; Gomyo S; Sekine I
Address: Department of Endodontics, School of Dentistry, Asahi University, Gifu, Japan.
Source: J Endod, 1995 Dec, 21:12, 592-3
Abstract: The effect of eliminating the smear layer by means of 15% EDTA solution as a root canal irrigant was studied in 189 single-rooted infected teeth. Each tooth was treated at two appointments, and the root canal bacteriological examination was studied on the first (pretreatment, and after enlargement and irrigation) and second (pretreatment) visits. The root canals were irrigated with 15% EDTA solution with ultrasonics agitation. No antibacterial intracanal medications were used between the appointment. When 15% EDTA solution was used, no bacteria could be recovered from 93 of 129 root canals at the sampling stage on second visit. No bacteria were found in 21 of 60 root canals when saline solution was used as an irrigant. These results suggest that 15% EDTA solution is more effective than saline solution as a root canal irrigant.
Language of Publication: English
Unique Identifier: 96177051

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MeSH Heading (Major): Anti-Infective Agents, Local|*PD; Dental Pulp Cavity|*MI; Disinfection|*MT; Edetic Acid|*PD; Root Canal Irrigants|*PD; Root Canal Preparation|*MT; Smear Layer|*
MeSH Heading: Bacteria, Anaerobic|DE/IP; Chi-Square Distribution; Colony Count, Microbial; Evaluation Studies; Follow-Up Studies; Human; Sodium Chloride|PD; Ultrasonic Therapy

Publication Type: JOURNAL ARTICLE
ISSN: 0099-2399
Country of Publication: UNITED STATES

Record 64 from database: MEDLINE
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Title: Renal and extrarenal clearance of 99mTc-MAG3: a comparison with 125I-OIH and 51Cr-EDTA in patients representing all levels of glomerular filtration rate.
Author: Rehling M; Nielsen BV; Pedersen EB; Nielsen LE; Hansen HE; Bacher T
Address: Department of Clinical Physiology and Nuclear Medicine, Skejby University Hospital, Aarhus, Denmark.
Source: Eur J Nucl Med, 1995 Dec, 22:12, 1379-84
Abstract: .This investigation was undertaken in order to determine whether the clearance of technetium-99m mercaptoacetyltriglycine (99mTc-MAG3) is more closely correlated to the clearance of iodine-125 orthoiodohippurate (125I-OIH) than to the clearance of chromium-51 ethylene diamine tetra-acetate (51Cr-EDTA) and whether there is a clinically significant extrarenal clearance of 99mTc-MAG3 . Fifty-one patients with a glomerular filtration rate (GFR) of 4-132ml/min were studied. After a simultaneous single injection of the three tracers, plasma clearance was measured from blood samples 0-5h post injection (p.i.) (0-24h in patients with GFR<15ml/min). Renal plasma clearance was measured 0-5h p.i. The ratio between the renal plasma clearance of 99mTc-MAG3 and 125I-OIH was 0.57. The ratio between the renal plasma clearance of 99mTc-MAG3 and 51Cr-EDTA was 2.57. The coefficient of variation (CV) of the 99mTc-MAG3/125I-OIH ratio was significantly smaller than the CV of the 99mTc-MAG3/51Cr-EDTA ratio (13.4% versus 31.2%). The corresponding plasma clearance ratios were 0.59 (CV=14.8%) and 2.48 (CV=27.0%). Plasma clearance overestimated renal plasma clearance by 7.0ml/min (5.4%) for 99mTc-MAG3 and by 4.1ml/min (8.4%) for 51Cr-EDTA. The difference in plasma and renal plasma clearance of 125I-OIH of 5.5ml/min did not reach statistical significance. Red blood cell binding of 99mTc-MAG3, 125I-OIH and 51Cr-EDTA was 2.0%, 14.6% and 0.2%, respectively. Protein binding of 99mTc-MAG3, 125I-OIH and 51Cr-EDTA was 86.3%, 61.1% and 5.9%, respectively. The volume of distribution of 99mTc-MAG3, 125I-OIH and 51Cr-EDTA was 16.3%, 27.0% and 19.4% of body weight. In conclusion, the clearances of 99mTc-MAG3 and 125I-OIH are more closely correlated than is the clearance of 99mTc-MAG3 with GFR. Extrarenal clearance of 99mTc-MAG3 is relatively smaller than extrarenal clearance of 51Cr-EDTA. Thus, plasma clearance of 99mTc-MAG3 can be used as a measure of renal tubular function.
Language of Publication: English
Unique Identifier: 96118877

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MeSH Heading (Major): Chromium Radioisotopes|DU/*PK; Edetic Acid|DU/*PK; Glomerular Filtration Rate|*; Iodine Radioisotopes|DU/*PK; Iodohippuric Acid|DU/*PK; Technetium Tc 99m Mertiatide|DU/*PK
MeSH Heading: Adult; Aged; Comparative Study; Female; Human; Kidney|ME/RI; Male; Middle Age; Support, Non-U.S. Gov't

Publication Type: JOURNAL ARTICLE
ISSN: 0340-6997
Country of Publication: GERMANY

Record 65 from database: MEDLINE
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Title: Analysis of 15 cases with platelet EDTA-dependent antibodies.
Author: Zupanska B; Maslanka K
Address: Department of Serology, Institute of Haematology & Blood Transfusion, Warsaw.
Source: Acta Haematol Pol, 1995, 26:4, 361-5
Abstract: In 15 patients with thrombocytopenia EDTA-dependent platelet antibodies (IgM agglutinins active in 20 degrees C) were detected. These antibodies were found in patients with autoimmune diseases, infections and neoplasma as well as in healthy persons (including pregnant woman). In 10 persons pseudothrombocytopenia (PTCP) was diagnosed since the low platelet counts were found only in EDTA-blood, and the patients did not have bleeding symptoms. In 5 other cases EDTA-dependent antibodies were not the only cause of thrombocytopenia since the low platelet counts were also observed in the citrate-blood and occassionally the bleeding symptoms occurred; in 3 of them in addition autoantibodies were suspected. Due to the proper diagnosis of PTCP, in five patients unnecessary corticotherapy was discontinued, one person avoided splenectomy, in two patients previously postponed surgery was performed and one person could be passed for employment.
Language of Publication: English
Unique Identifier: 96151572

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MeSH Heading (Major): Autoantibodies|*AN; Blood Platelets|DE/*IM; Edetic Acid|*PD; Thrombocytopenia|*CI/IM
MeSH Heading: Adult; Female; Human; Immunoglobulins|IM; Platelet Aggregation|DE; Platelet Count|DE; Pregnancy

Publication Type: JOURNAL ARTICLE
ISSN: 0001-5814
Country of Publication: POLAND

Record 66 from database: MEDLINE
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Title: A novel method for isolation of Chlamydia pneumoniae by treatment with trypsin or EDTA.
Author: Kazuyama Y; Lee SM; Amamiya K; Taguchi F
Address: Research and Development Center of Hygienic Science, Kitasato University, Minatoku, Tokyo, Japan.
Source: J Clin Microbiol, 1997 Jun, 35:6, 1624-6
Abstract: To establish a novel method for the efficient isolation of Chlamydia pneumoniae, experiments were performed to determine the effects of EDTA or trypsin treatment of C. pneumoniae on its adsorption and inclusion body formation. Treatment of C. pneumoniae with 0.1% trypsin or 1 mM EDTA significantly increased inclusion body-forming activity from 8,000- to 10,000-fold higher than that of the control. C. pneumoniae was successfully isolated in cultured cells which were inoculated with clinical specimens after treatment with 0.1% trypsin.
Language of Publication: English
Unique Identifier: 97306033

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MeSH Heading (Major): Bacteriological Techniques|*; Chlamydia pneumoniae|DE/GD/*IP; Edetic Acid|*/PD; Trypsin|*/PD
MeSH Heading: Cell Line; Chelating Agents|PD; DNA, Bacterial|AN; Ear, Middle|MI; Human; Inclusion Bodies|DE; Lung; Pharynx|MI; Polymerase Chain Reaction|MT; Sputum|MI

Publication Type: JOURNAL ARTICLE
ISSN: 0095-1137
Country of Publication: UNITED STATES

Record 67 from database: MEDLINE
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Title: Reproducibility of simplified techniques for the measurement of 51Cr-EDTA clearance.
Author: Piepsz A; Tondeur M; Ham HR
Address: Department of Radioisotopes, HÈopital Saint-Pierre, Brussels, Belgium.
Source: Nucl Med Commun, 1996 Dec, 17:12, 1065-7
Abstract: The aim of this study was to assess the reproducibility of a number of simplified clearance methods using chromium-51 ethylenediamine tetraacetic acid (51Cr-EDTA) and to compare these with the multiple blood sample technique. Twenty-four healthy volunteers were enrolled into the study. The test was performed twice, 1 week apart and under similar physiological conditions. After intravenous injection of the tracer, 13 blood samples were taken between 5 and 240 min. The reference clearance was calculated after adapting a bi-exponential fit on the whole plasma disappearance curve. Simplified methods included the slope intercept method using different combinations of blood samples (2 and 4 h; 2, 3 and 4 h; 1.5, 2, 3 and 4 h; 1.5, 2 and 3 h) and several single clearance methods (Constable 3 h; Groth 3 h; Groth 4 h). The standard deviation of the differences between the first and second measurements were similar for all methods, confirming the comparable reproducibility of these methods.
Language of Publication: English
Unique Identifier: 97158025

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MeSH Heading (Major): Chromium Radioisotopes|BL/*DU/PK; Edetic Acid|*DU/PK
MeSH Heading: Comparative Study; Evaluation Studies; Human; Metabolic Clearance Rate; Reference Values; Reproducibility of Results

Publication Type: JOURNAL ARTICLE
ISSN: 0143-3636
Country of Publication: ENGLAND

Record 68 from database: MEDLINE
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Title: How good is the slope of the second exponential for estimating 51Cr-EDTA renal clearance?
Author: Piepsz A; Ham HR
Address: HÈopital Saint Pierre, Brussels, Belgium.
Source: Nucl Med Commun, 1997 Feb, 18:2, 139-41
Abstract: The aim of this study was to test the reproducibility of the second exponential of the plasma disappearance curve of 51Cr-EDTA and to compare it with the single slope-intercept method. The test was performed twice among 24 young healthy volunteers at an interval of 8 days and under similar physiological conditions. After the intravenous injection of 3.7 MBq of 51Cr-EDTA, four blood samples were taken between 1.5 and 4 h and combinations of two, three or all four of these were used to calculate the slope of the plasma clearance curve. The clearances rates were calculated using these slopes and the corresponding intercepts of the slopes with the y-axis. The standard deviation of the differences between the first and the second measurements was between 8 and 10% for the different clearance estimates, and between 11 and 17% for the different slope estimates, reflecting the wider variability of the slope estimate. Using the first series of measurements, the change of timing for blood sampling introduced a wider variability for the slope estimate than for the clearance estimate.
Language of Publication: English
Unique Identifier: 97231374

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MeSH Heading (Major): Chromium Radioisotopes|BL/*PK; Edetic Acid|AN/*PK; Kidney|*PH; Kidney Function Tests|*MT
MeSH Heading: Adult; Comparative Study; Human; Metabolic Clearance Rate; Reproducibility of Results; Time Factors

Publication Type: JOURNAL ARTICLE
ISSN: 0143-3636
Country of Publication: ENGLAND

Record 69 from database: MEDLINE
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Title: The cytotoxic interaction of inorganic trace elements with EDTA and cisplatin in sensitive and resistant human ovarian cancer cells.
Author: Maier RH; Purser SM; Nicholson DL; Pories WJ
Address: Department of Surgery, School of Medicine, East Carolina University, Greenville, North Carolina 27858-4354, USA.
Source: In Vitro Cell Dev Biol Anim, 1997 Mar, 33:3, 218-21
Abstract: Cisplatin (CDDP)-sensitive and -resistant human ovarian cells were studied in vitro with the objective of enhancing CDDP cytotoxicity by the addition of a metal and the chelate ethylenediaminetetraacetic acid (EDTA), to the CDDP. Chelateable elements, such as bismuth, calcium, cadmium, copper, iron, magnesium, selenium, vanadium, and zinc, when added to CDDP and in the presence of EDTA increased the cytotoxicity of the CDDP as compared to CDDP treatment alone.
Language of Publication: English
Unique Identifier: 97266338

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MeSH Heading (Major): Antineoplastic Agents|*PD; Cell Death|*; Cisplatin|*PD; Edetic Acid|*PD; Ovarian Neoplasms|*PA; Trace Elements|*PD
MeSH Heading: Calcium|PD; Chelating Agents|PD; Drug Resistance, Neoplasm; Female; Human; Magnesium|PD; Tumor Cells, Cultured

Publication Type: JOURNAL ARTICLE
ISSN: 1071-2690
Country of Publication: UNITED STATES

Record 70 from database: MEDLINE
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Title: Removing IgG antibodies from intact red cells: comparison of acid and EDTA, heat, and chloroquine elution methods.
Author: Burin des Roziers N; Squalli S
Address: Etablissement de Transfusion Sanguine de Languedoc-Roussillon, Nimes, France.
Source: Transfusion, 1997 May, 37:5, 497-501
Abstract: BACKGROUND: To accurately phenotype red cell from patients with a positive direct antiglobulin test (DAT), nonlytic elution procedures were assessed for their ability to dissociate IgG from antibody-coated red cells without altering red cell antigen expression. STUDY DESIGN AND METHODS: Antibodies coating red cells that were sensitized in vivo (warm-reactive autoantibodies: 8 patients) or in vitro (42 alloantibodies) were eluted by using glycine-HCl and EDTA (acid/ EDTA), heat (56 degrees C, 10 min), or chloroquine method. RESULTS: Acid/EDTA elution gave the best results, reducing DAT positivity to microscopic levels or rendering the DAT negative in 48 of 50 instances, whereas 4 samples remained resistant to heat elution and 24 to chloroquine. Standard DAT agglutination scores demonstrated that both acid/EDTA and heat elution were superior to the chloroquine method (p < 0.0001). With the gel low-ionic-strength saline indirect antiglobulin test, acid/ EDTA was superior to heat (p < 0.001). Overall, acid/ EDTA elution dissociated more antibodies than heat (p < 0.0001), especially for Kell system (K, k, Kpa, Kpb) alloantibodies. Common red cell antigens, other than Kell system antigens, were unaffected by acid/EDTA elution. In contrast, the expression of most blood group antigens was diminished after heat elution. However, it was possible to type red cell antigens by using gel low-ionic-strength saline indirect antiglobulin tests or tube agglutination methods. CONCLUSION: Although heat elution may be used on a limited basis, the acid/EDTA method appears to be the procedure of choice for typing red cell coated with warm-reactive IgG alloantibodies or autoantibodies.
Language of Publication: English
Unique Identifier: 97293880

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MeSH Heading (Major): Antibodies|*IP; Erythrocytes|*IM
MeSH Heading: Acids|PD; Autoantibodies|IP; Chloroquine|PD; Comparative Study; Coombs' Test|MT; Edetic Acid|PD; Heat; Human; IgG|IM; Methods

Publication Type: JOURNAL ARTICLE
ISSN: 0041-1132
Country of Publication: UNITED STATES

Record 71 from database: MEDLINE
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Title: Flow cytometric evaluation of platelet activation in blood collected into EDTA vs. Diatube-H, a sodium citrate solution supplemented with theophylline, adenosine, and dipyridamole [see comments]
Author: Kühne T; Hornstein A; Semple J; Chang W; Blanchette V; Freedman J
Address: Department of Immunohaematology, St. Michael's Hospital, Toronto, Canada.
Source: Am J Hematol, 1995 Sep, 50:1, 40-5
Abstract: With platelet activation, there is modulation of platelet surface molecule expression. In flow cytometric analyses of in vivo platelet activation, results are often confounded by activation induced in vitro by the preparative procedures. It is particularly important therefore to prevent or retard platelet activation as soon as possible after withdrawal of the blood sample. Taking blood into paraformaldehyde, or fixing the cells with paraformaldehyde as soon as possible after withdrawal, has been employed to prevent platelet activation in vitro, but paraformaldehyde-fixed platelets cannot be further used in functional studies. We investigated the efficacy of Diatube-H, a commercially available combination of platelet antagonists (theophylline, adenosine, and dipyridamole), in preventing or retarding platelet activation in vitro, along with its effects on modulation of platelet membrane glycoproteins (GP) and adhesion molecules. In contrast to blood taken into EDTA, blood taken into Diatube-H vacutainer tubes could be stored at room temperature for up to 4 hr prior to paraformaldehyde fixation without significant in vitro platelet activation, as measured by CD62P, CD63 and modulation of GPIb and GPIIbIIIa surface expression. Hence, paraformaldehyde fixation could be deferred for several hours, permitting transport of samples from distant sites. Studies of thrombin-induced platelet activation indicated that platelets taken into Diatube-H remained functional i.e. were able to be activated. Expression of the CD29, CD49b and CD31 adhesion molecules on the platelet surface was unaffected by storage in Diatube-H. The results suggest that Diatube-H may be a useful reagent for flow cytometric studies of platelets when the samples cannot be processed immediately.
Language of Publication: English
Unique Identifier: 95397778

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MeSH Heading (Major): Anticoagulants|*; Citrates|*; Flow Cytometry|*MT; Platelet Activation|*
MeSH Heading: Adenosine; Adult; Comparative Study; Dipyridamole; Edetic Acid; Female; Human; Male; Solutions; Theophylline

Publication Type: JOURNAL ARTICLE
ISSN: 0361-8609
Country of Publication: UNITED STATES

Record 72 from database: MEDLINE
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Title: Report on management of renale failure in Europe, XXVI, 1995. Rare diseases in renal replacement therapy in the ERA-EDTA Registry.
Author: Tsakiris D; Simpson HK; Jones EH; Briggs JD; Elinder CG; Mendel S; Piccoli G; dos Santos JP; Tognoni G; Vanrenterghem Y; Valderrabano F
Address
Source: Nephrol Dial Transplant, 1996, 11 Suppl 7:, 4-20
Abstract: The proportion of centres returning the ERA-EDTA Registry questionnaires has decreased considerably in recent years. Demographic information, based on the response rate of centres in 1994 (44%), does not allow reasonable projections for management of renal failure in Europe. To encourage the participation of non-responding centres, the timing was right to show the powerful impact of the ERA-EDTA Registry as a supra-national registry, by studying patients in renal replacement therapy (RRT) suffering from rare diseases. Four such diseases, Fabry's disease, nephropathy due to cyclosporin (CsA), nephropathy due to cisplatin and scleroderma, were studied using the records of 440665 patients on file up to 31 December 1993. There were 83 patients with Fabry's disease (0.0188%), 85 patients with CsA nephropathy (0.0193%), 120 patients with cisplatin nephropathy (0.0272%) and 625 patients with scleroderma (0.142%). Scleroderma was introduced as a primary renal disease (PRD) in the ERA-EDTA Registry in 1977. Seven patients were accepted for RRT in that year, whereas the number increased to over 50 new patients per year after 1986. More than half of the patients were aged over 55 years, and 68% of them were women. Survival rate of dialysis patients suffering from scleroderma was 22% at 5 years, compared to 51% in patients with standard primary renal diseases. The main causes of death were cardiovascular complications (41%), cachexia (15%) and infection (10%). Survival of first graft in a small number of 28 patients was 44% at 3 years, compared to 60% in standard PRD. Patient survival after first transplant, however, was higher by 32% at 3 years compared to that of dialysis patients. Cisplatin nephropathy was introduced as a PRD in the ERA-EDTA Registry in 1985, and since then six to 19 new patients have been accepted for RRT each year. The main reason for undergoing cisplatin treatment was ovarian (32%) and testicular cancer (21%), and the mean interval from treatment to RRT was 21.5 months, ranging widely from 0.1 to 131 months. Patient survival on dialysis was 22% at 5 years, compared to 51% in patients with standard PRD. Malignancy and cachexia accounted for over 60% of the total number of deaths. CsA nephropathy was introduced as a PRD in the ERA-EDTA Registry in 1985 and, despite its rarity, is of particular interest as a new iatrogenic entity resulting from CsA administration, mainly in solid organ transplantation. In 1985, two new patients commenced RRT in Europe, and the number increased to 59 in 1991-93. The main reason for undergoing CsA treatment was heart (68%) and liver transplant (22%), and the mean interval from treatment to RRT was 50.2 months, ranging from 5 to 90 months. Patient survival on dialysis was 46% at 4 years, compared to 58% in patients with standard primary nephropathies. Cardiovascular causes (48%) and infection (17%) were the main causes of death. Fabry's disease was introduced as a PRD in the ERA-EDTA Registry in 1985, and since the four to 13 new patients per year have commenced RRT in Europe. It is a sex-linked recessive disorder primarily affecting males (87%), and the mean age at start of RRT was 38 years. Proteinuria, skin lesions and painful paresthesiae were the most common presenting symptoms, and over 70% of the patients were hypertensive and had significant cardiovascular problems at RRT. Patient survival on dialysis was 41% at 5 years, compared to 68% in patients with standard primary nephropathies. Cardiovascular complications (48%) and cachexia (17%) were the main causes of death. Graft survival at 3 years in 33 patients was not inferior to that of patients with standard nephropathies (72% vs 69%), and patient survival after transplantation was comparable to that of patients under 55 years of age with standard PRD. (ABSTRACT TRUNCATED)
Language of Publication: English
Unique Identifier: 97220821

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MeSH Heading (Major): Kidney Diseases|CI/*EP/*TH; Registries|*; Renal Replacement Therapy|*/MO/SN
MeSH Heading: Adolescence; Adult; Aged; Cisplatin|AE; Cyclosporine|AE; Europe|EP; Fabry's Disease|EP/MO/TH; Female; Human; Male; Middle Age; Scleroderma, Systemic|EP/MO/TH; Survival Rate

Publication Type: JOURNAL ARTICLE
ISSN: 0931-0509
Country of Publication: ENGLAND

Record 73 from database: MEDLINE
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Title: Metal excretion and magnesium retention in patients with intermittent claudication treated with intravenous disodium EDTA.
Author: Guldager B; J‡rgensen PJ; Grandjean P
Address: Department of Surgery, Hiller‡d Hospital, Denmark.
Source: Clin Chem, 1996 Dec, 42:12, 1938-42
Abstract: Sixty patients with intermittent claudication participated in a double-blind placebo-controlled trial of 20 courses of intravenous chelation therapy with 3 g of disodium EDTA vs placebo during 5-9 weeks. After the first infusion, the 24-h urinary excretion of lead and zinc was approximately 25-fold higher in the EDTA-treated group; relative differences for copper and calcium were smaller. Urinary magnesium excretion in the EDTA-treated group was one-third less than in the control group. After the treatment period, the blood lead concentration had decreased by approximately 73% and the serum zinc concentration by approximately 34%; other changes in blood concentrations were negligible. The loss of essential minerals and the possible redistribution of lead in the body may constitute a disadvantage that should be taken into account in repeated intravenous EDTA treatment.
Language of Publication: English
Unique Identifier: 97124459

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MeSH Heading (Major): Edetic Acid|*TU; Intermittent Claudication|*DT/ME; Magnesium|BL/*ME/UR; Metals|*UR
MeSH Heading: Adult; Aged; Aged, 80 and over; Calcium|UR; Copper|BL/UR; Double-Blind Method; Human; Lead|BL/UR; Mercury|BL; Middle Age; Placebos; Support, Non-U.S. Gov't; Zinc|BL/UR

Publication Type: CLINICAL TRIAL; JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
ISSN: 0009-9147
Country of Publication: UNITED STATES

Record 74 from database: MEDLINE
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Title: Smear removal and collagen exposure after non-surgical root planing followed by etching with an EDTA gel preparation.
Author: Blomlöf JP; Blomlöf LB; Lindskog SF
Address: Department of Oral Histology and Cell Biology, School of Dentistry, Karolinska Institutet, Stockholm, Sweden.
Source: J Periodontol, 1996 Sep, 67:9, 841-5
Abstract: Low pH aqueous solutions of citric acid have been used in surgical periodontal therapy mainly for two reasons. It dissolves smear after a relatively short exposure time and it has been claimed to selectively remove root surface-associated mineral exposing collagen to varying degrees. The use of low pH etching agents has recently been challenged in both experimental in vitro and in vivo studies based on their necrotizing effect on the surrounding periodontal tissues. The purpose of the present study was to assess the efficacy of subgingival application of an EDTA gel preparation in removing smear and exposing collagen fibers in root surfaces following non-surgical periodontal therapy. Root surfaces which were root planed did not reveal any patent dentinal tubuli or collagen fibers. This was in contrast to the findings seen after root planing followed by etching, where intertubular surfaces were covered by a dense fibrillar network extending into patent dentinal tubuli. In high magnification these intertubular fibers displayed the cross-striated texture typical for collagen fibers. Etching of root planed surfaces with EDTA may thus enhance the effect of non-surgical root debridement in the same way as has been shown during experimental surgical procedures.
Language of Publication: English
Unique Identifier: 97039058

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MeSH Heading (Major): Acid Etching, Dental|*MT; Edetic Acid|*PD; Periodontal Diseases|*TH; Root Planing|*; Smear Layer|*; Tooth Root|*DE
MeSH Heading: Collagen|CH/PH; Dentin|DE; Erythrocytes|DE; Human; Hydrogen-Ion Concentration; Protein Conformation|DE; Surface Properties; Trypsin|PD; Wound Healing

Publication Type: JOURNAL ARTICLE
ISSN: 0022-3492
Country of Publication: UNITED STATES

Record 75 from database: MEDLINE
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Title: EDTA-dependent pseudothrombocytopenia: a clinical and epidemiological study of 112 cases, with 10-year follow-up.
Author: Bizzaro N
Address: Laboratory of Clinical Pathology, Ospedale Civile, Venice, Italy.
Source: Am J Hematol, 1995 Oct, 50:2, 103-9
Abstract: In the past 10 years, we have observed 112 cases of EDTA-dependent pseudothrombocytopenia (PTCP) due to in vitro platelet clumping at room temperature. 93 patients had antiplatelet antibodies (48 IgM, 30 IgG, 3 IgA, and 12 had two different isotypes concomitantly). In 20% of patients, the presence of IgM antibodies characteristically accompanied platelet agglutination also at 37 degrees C, and in citrated blood. The phenomenon was not age or sex related, nor was it associated with any particular pathology or use of specific drugs, and was present in both healthy subjects and patients with various diseases. Flow cytofluorimetric analysis of CD5-positive B cells, which are responsible for autoantibody production, did not demonstrate any changes in the percentage and absolute number of this lymphocyte subset. Average follow-up was 5 years (6 months-10 years); however, previous clinical records disclosed that PTCP was present for more than 15 years in four cases, and more than 20 years in three others, with no clinical manifestation of disease. This study confirms that EDTA-dependent PTCP is a phenomenon related to the presence of natural autoantibodies with antiplatelet activity, devoid of pathological significance. Its clinical interest resides in the need for its prompt and certain recognition in order to avoid unnecessary examinations and therapeutic interventions. The best and most rapid technique for obtaining accurate platelet counts in PTCP subjects is to collect and examine EDTA blood at 37 degrees C; however, clumping will still be present in about 20% of these cases, and even in citrated blood. To obviate this phenomenon, blood should be collected in ammonium oxalate, and platelets counted in a Burker chamber.
Language of Publication: English
Unique Identifier: 96016357

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MeSH Heading (Major): Autoantibodies|*BL; Blood Platelets|*IM; Edetic Acid|*PD; Platelet Aggregation|*IM; Platelet Count|*; Thrombocytopenia|*IM
MeSH Heading: Adolescence; Adult; Aged; Aged, 80 and over; Antigens, CD5|AN; Autoimmune Diseases|BL; B-Lymphocytes|IM; Child; False Negative Reactions; Female; Follow-Up Studies; Human; IgA|BL; IgG|BL; IgM|BL; Lymphoproliferative Disorders|BL; Male; Middle Age; Oxalates

Publication Type: JOURNAL ARTICLE
ISSN: 0361-8609
Country of Publication: UNITED STATES

Record 76 from database: MEDLINE
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Title: Measurement of glomerular filtration rate by single-injection, single-sample techniques, using 51Cr-EDTA or iohexol.
Author: Rydström M; Tengström B; Cederquist I; Ahlmén J
Address: Department of Clinical Chemistry, County Central Hospital, SkÂovde, Sweden.
Source: Scand J Urol Nephrol, 1995 Jun, 29:2, 135-9
Abstract: Clearance calculations based on single sample determinations from 51Cr-EDTA samples 180 min after giving radioisotope showed a correlation coefficient of 0.992 to clearance calculations after multiple sampling of 51Cr-EDTA. The range of clearance determinations in 108 patients varied from 4 to 141 ml/min, 1.73 m2 BA. Twenty patients had clearance values below 20 ml/min, 1.73 m2 BA. Clearance calculations from single samples of iohexol from 180 min values showed a correlation coefficient of 0.986 to clearance calculations after multiple sampling of 51Cr-EDTA. These calculations were based on samples from 122 patients with clearance values varying from 4 to 139 ml/min, 1.73 m2 BA. No adverse reactions were registered during the study. This study confirms the reliability of single sample methods for clearance calculations at all levels of renal function.
Language of Publication: English
Unique Identifier: 96023721

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MeSH Heading (Major): Chromium Radioisotopes|*DU; Contrast Media|*; Edetic Acid|*DU; Glomerular Filtration Rate|*PH; Iohexol|*DU; Kidney Failure, Chronic|BL/*DI
MeSH Heading: Adult; Comparative Study; Female; Gamma Cameras; Human; Male; Reference Values; Reproducibility of Results

Publication Type: JOURNAL ARTICLE
ISSN: 0036-5599
Country of Publication: SWEDEN

Record 77 from database: MEDLINE
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Title: More on false thrombocytopenias: EDTA-dependent pseudothrombocytopenia associated with a congenital platelet release defect.
Author: Silvestri F; Masotti A; Pradella P; Zaja F; Barillari G; De Marco L
Address: Division of Haematology, University Hospital, Udine, Italy.
Source: Vox Sang, 1996, 71:1, 27-9
Abstract: We report herein the case of a 54-year-old woman with a moderate bleeding tendency, diagnosed as an EDTA-dependent pseudothrombocytopenia associated with a congenital platelet release defect. The patient, at the age of 12, had a misleading diagnosis of idiopathic thrombocytopenic purpura and all the recurrent bleeding problems she had during her life were referred to that disease. The recent correct diagnosis of a false thrombocytopenia stimulated further laboratory investigation on the cause of the patient's bleeding tendency with the consequent identification of a congenital platelet deficiency of the arachidonic acid pathway. This finding is of relevant importance for the management of the patient in case of elective surgery or hemorrhagic emergency.
Language of Publication: English
Unique Identifier: 96434386

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MeSH Heading (Major): Blood Coagulation Disorders|*DI; Blood Platelets|*ME; Platelet Aggregation|*; Thrombocytopenia|*DI
MeSH Heading: Arachidonic Acid|BL; Case Report; Edetic Acid; Female; Human; Middle Age

Publication Type: JOURNAL ARTICLE
ISSN: 0042-9007
Country of Publication: SWITZERLAND

Record 78 from database: MEDLINE
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Title: C-reactive protein: the difference between quantitation is serum and EDTA plasma.
Author: Nordin G; Samuelsson I; Andersson B; Börjeson J
Address: Department of Clinical Chemistry, Lasarettet, Helsingborg, Sweden.
Source: Scand J Clin Lab Invest, 1996 Apr, 56:2, 123-7
Abstract: We report the differences between using either EDTA plasma or serum in a turbidimetric assay for quantitation of C-reactive protein (CRP). A systematic discrepancy was found for these two sample materials. This was most pronounced in the low concentration range (below 20 mg1(-1)) at which lower values were found in serum than in EDTA plasma. Conversely, in the high concentration range, serum showed slightly higher values. Addition of K3-EDTA to the reaction buffer improved the kinetics for sera with low concentrations of CRP, thus increasing the sensitivity of the assay. We found an overall constant discrepancy of approximately 8% lower values in plasma than in serum (equally for low and high levels of CRP) after the addition of K3-EDTA. The most probable explanation for this effect seems to be the differing water content of serum and EDTA plasma. We discuss the role and function of EDTA in the CRP assay and suggest some hypothetical mechanisms.
Language of Publication: English
Unique Identifier: 96295062

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MeSH Heading (Major): Blood Chemical Analysis|*MT/ST; C-Reactive Protein|*AN/CH/ST; Edetic Acid|*
MeSH Heading: Buffers; Comparative Study; Human; Nephelometry and Turbidimetry; Reference Standards; Reference Values; Specimen Handling

Publication Type: JOURNAL ARTICLE
ISSN: 0036-5513
Country of Publication: NORWAY

Record 79 from database: MEDLINE
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Title: Production and characterization of monoclonal antibodies to the EDTA extract of Leptospira interrogans, serovar icterohaemorrhagiae.
Author: Leite LT; Resende M; de Souza W; Camargos ER; Koury MC
Address: Departmento de Microbiologia e Morfologia do Instituto de Ciencias BiolÆgicas, Universidade Federal de Minas Gerais, Belo Horizonte.
Source: Rev Soc Bras Med Trop, 1996 Sep, 29:5, 483-9
Abstract: Monoclonal antibodies (MABs) were produced against an ethylenediaminetetraacetate (EDTA) extract of Leptospira interrogans serovar icterohaemorrhagiae being characterized by gel precipitation as IgM and IgG (IgG1 and IgG2b). The EDTA extract was detected as several bands by silver staining in SDS-PAGE. In the Western blot the bands around 20 KDa reacted with a monoclonal antibody, 47B4D6, and was oxidized by periodate and was not digested by pronase, suggesting that the determinant is of carbohydrate nature. Immunocytochemistry, using colloidal gold labeling, showed that an EDTA extract determinant recognized by monoclonal antibody 47B4D6, is localized under the outer envelope of serovar icterohaemorrhagiae. The MAB raised against the EDTA extract was not able to protect hamsters from lethal challenge with virulent homologous leptospires.
Language of Publication: English
Unique Identifier: 97040290

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MeSH Heading (Major): Antibodies, Monoclonal|*IM; Bacterial Outer Membrane Proteins|*IM/IP; Leptospira interrogans|*IM; Lipopolysaccharides|*IM/IP
MeSH Heading: Animal; Edetic Acid; Female; Hamsters; Human; IgG|IM; IgM|IM; Immunization, Passive; Mesocricetus; Mice; Mice, Inbred BALB C; Support, Non-U.S. Gov't

Publication Type: JOURNAL ARTICLE
ISSN: 0037-8682
Country of Publication: BRAZIL

Record 80 from database: MEDLINE
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Title: EDTA chelation therapy in the treatment of vascular disease [see comments]
Author: Chappell LT; Janson M
Address: Wright State School of Medicine, Dayton, Ohio, USA.
Source: J Cardiovasc Nurs, 1996 Apr, 10:3, 78-86
Abstract: Ethylenediamine tetraacetic acid (EDTA) chelation therapy has been used for decades for the treatment of vascular disease, alone or in combination with other treatments. This article includes a historic review of the research literature, current evidence of effectiveness, potential mechanisms of action of EDTA, and some brief case reports. The authors conclude that EDTA chelation therapy is a valuable therapeutic option for vascular disease, either alone or in conjunction with standard treatment protocols.
Language of Publication: English
Unique Identifier: 96417531

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MeSH Heading (Major): Chelating Agents|*TU; Chelation Therapy|*; Edetic Acid|*TU; Vascular Diseases|*DT
MeSH Heading: Alternative Medicine; Human

Publication Type: JOURNAL ARTICLE; REVIEW; REVIEW, TUTORIAL
ISSN: 0889-4655
Country of Publication: UNITED STATES

Record 81 from database: MEDLINE
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Title: Plasma clearance rate of 51Cr-EDTA provides a precise and convenient technique for measurement of glomerular filtration rate in diabetic humans.
Author: Sambataro M; Thomaseth K; Pacini G; Robaudo C; Carraro A; Bruseghin M; Brocco E; Abaterusso C; DeFerrari G; Fioretto P; Maioli M; Tonolo GC; Crepaldi G; Nosadini R
Address: Institute of Internal Medicine, University of Padova, Italy.
Source: J Am Soc Nephrol, 1996 Jan, 7:1, 118-27
Abstract: It has not yet been fully clarified whether the plasma or renal clearance approach is the most reliable to investigate GFR in humans. The study presented here aimed to compare plasma decay with renal clearance of 51Cr-EDTA in 27 diabetic patients with patterns of renal function broadly dispersed in a wide range of values. Moreover, the comparison was also performed with renal clearance of nonlabeled iothalamate in a subgroup of 17 patients. A biexponential function was found to fulfill statistical and heuristic criteria for the modeling analysis of plasma 51Cr-EDTA decay with 19 samples after bolus intravenous 51Cr-EDTA injection. Individual GFR values from 51Cr-EDTA plasma clearance highly correlated with those from renal clearance (r2 = 0.977, P < 0.0001), but resulted on average about 2.5 mL.min-1.1.73 m-2 higher (66.8 +/- 6.5 mL.min-1.1.73 m-2 (mean +/- SE) versus 64.3 +/- 6.4, P < 0.02). This difference remained relatively constant from patients with normal renal function to those with impaired renal function, suggesting that the plasma clearance is slightly less accurate than renal clearance approach because of a constant extrarenal clearance rate. In the subgroup studied, a similar difference was found between GFR values from 51Cr-EDTA plasma clearance (84.7 +/- 7.3) and renal clearance of iothalamate (82.8 +/- 7.3), although not statistically significant (P = 0.4). Individual GFR values well correlated (r2 = 0.913, P < 0.0001). The precision and reproducibility of the experimental approaches were assessed by comparing three coefficients of variation: (1) CVb of the bolus injection, because of measurement errors; (2) CVc of the continuous infusion, which additionally includes errors of urine volume measurement and physiological variability in the same day; and (3) CVr of repeated measurements by using bolus injection, which also accounts for physiological variability in different days. CVc of iothalamate and 51Cr-EDTA infusions were 7.5 +/- 1.9% and 7.4 +/- 1.2% respectively. CVb and CVr of bolus injection of 51Cr-EDTA were 2.6 +/- 0.3% and 3.5 +/- 0.8% respectively. CVb and CVr of bolus injection of 51Cr-EDTA, but not CVc of iothalamate and 51Cr-EDTA infusions were twofold to tenfold lower than the percent yearly change reported in IDDM and NIDDM patients. More particularly, CVr was significantly less than CVc. In order to make the test less cumbersome, a reduced sampling schedule with seven samples was designed and validated. GFR measured with seven samples was 66.1 +/- 6.4 (P = 0.1 when compared with the full 19-sample schedule) with a CVb of 3.5 +/- 0.5%. This seven-sample protocol was not different from that obtained with the previously described simplified method of Br‡chner-Mortensen (63.9 +/- 6.8, P = 0.16), yet yielding a statistically more accurate estimate (coefficient of variation for Br‡chner-Mortensen method = 12.1 +/- 2.9, P = 0.004). Moreover, only bolus injection, along with modeling analysis of plasma clearance rate, allows the accurate measurement of the extracellular fluid volume, an important parameter in diabetic patients. It was concluded that the reduced seven-plasma sample protocol is able to detect as small as 4 to 5% changes per year in a single patient. Moreover, it provides precise and accurate estimate of GFR in diabetic patients with hyperfiltration, who are postulated to be at higher risk to develop renal damage.
Language of Publication: English
Unique Identifier: 96403840

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MeSH Heading (Major): Diabetes Mellitus|BL/*PP; Edetic Acid|*PK; Glomerular Filtration Rate|*PH
MeSH Heading: Chromium Radioisotopes|ME; Female; Human; Infusions, Intravenous; Iothalamic Acid|PK; Male; Middle Age; Reproducibility of Results

Publication Type: JOURNAL ARTICLE
ISSN: 1046-6673
Country of Publication: UNITED STATES

Record 82 from database: MEDLINE
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Title: Survival modelling in kidney transplantation: hazard rates of graft loss. Transplant Working Group of the Registry Committee of the European Dialysis and Transplantation Association-European Renal Association (EDTA-ERA).
Author: Landais P; Jais JP; Margreiter R; Salmela K; Brunner F; Selwood N; Short C
Address: Laboratoire de Biostatistique et d'Informatique MÆedicale, HÈopital Necker-Enfants Malades, Paris, France.
Source: Nephrol Dial Transplant, 1995, 10 Suppl 1:, 90-4
Abstract: Mathematical modelling of survival data provides long-term projection of graft survival and allows evaluation of the impact of several variables on graft outcome. We analysed 52,315 first cadaveric grafts performed between 1971 and 1985 and reported to the EDTA Registry. We quantified the risk of graft loss using the hazard rates. The hazard function provides the magnitude of the risk of graft loss at a given time post-transplantation. For the 1971 and the 1985 cohorts, the risk of graft loss at 1 month posttransplantation was 143 and 53 per 1000 patient-months, respectively. At 1 year it was 5 and 2.4 per 1,000, respectively. The hazard function thus allowed quantification of the magnitude of the risk of graft loss and its evolution along with time. At 5 years posttransplantation, the risk of graft loss was close to 1 per 1,000, whatever the cohort considered, quantifying the lack of improvement of graft losses in the long term since the early 1970s. It was also possible to evaluate the composition of the risk and to explore the respective influence of graft failure and of patient death.
Language of Publication: English
Unique Identifier: 95342509

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MeSH Heading (Major): Graft Rejection|*MO; Kidney Transplantation|*MO; Models, Theoretical|*
MeSH Heading: Aged; Female; Graft Survival; Human; Incidence; Kidney Diseases|SU; Male; Retrospective Studies; Risk Factors; Sex Factors; Survival Analysis

Publication Type: JOURNAL ARTICLE
ISSN: 0931-0509
Country of Publication: ENGLAND

Record 83 from database: MEDLINE
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Title: Malignancies after renal transplantation: the EDTA-ERA registry experience. European Dialysis and Transplantation Association-European Renal Association.
Author: Brunner FP; Landais P; Selwood NH
Address: St. Thomas Hospital.
Source: Nephrol Dial Transplant, 1995, 10 Suppl 1:, 74-80
Abstract: The incidence of malignancies in recipients of renal transplants was compared to that in non-grafted patients on maintenance dialysis as reported to the EDTA-ERA Registry and in the general population as recorded by the cancer registries of England and Wales, of Sweden, of the (former) German Democratic Republic, and of Lombardy and Varese in Northern Italy. For tumours known to be associated with immunosuppression, namely Kaposi's sarcoma, non-Hodgkin lymphoma and the common malignancies of the skin (except melanoma), an increased incidence was confirmed for the transplanted population. Thyroid carcinoma and hepatoma were found to be more frequent in non-grafted patients on dialysis as well as after renal transplantation. An increased incidence of cancer of the cervix and of the body of the uterus was recorded only for young cohorts with a functioning graft but not for women after menopause. Most of the other malignancies had similar incidences in grafted and non-grafted populations which did not differ from those in the general populations of the cancer registries except cancer of the colon which was slightly more frequent, particularly at 10-20 years after the first transplant operation. Survival after diagnosis of cancer at the most frequent sites, such as bronchopulmonary, breast, oesophagogastric and colorectal cancer, did not differ between non-grafted patient groups on dialysis and those who developed the tumour while carrying a functioning renal transplant.(ABSTRACT TRUNCATED AT 250 WORDS)
Language of Publication: English
Unique Identifier: 95342506

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MeSH Heading (Major): Kidney Transplantation|*; Neoplasms|*EP; Postoperative Complications|*EP
MeSH Heading: Age Distribution; Female; Human; Immunosuppressive Agents|TU; Incidence; Kidney Failure, Chronic|SU; Male; Middle Age; Registries; Retrospective Studies; Sex Distribution; Survival Rate

Publication Type: JOURNAL ARTICLE
ISSN: 0931-0509
Country of Publication: ENGLAND

Record 84 from database: MEDLINE
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Title: Transplantation Report. 2: Pre-emptive renal transplantation in adults aged over 15 years. The EDTA-ERA Registry. European Dialysis and Transplant Association-European Renal Association.
Author: Berthoux FC; Jones EH; Mehls O; Valderrábano F
Address
Source: Nephrol Dial Transplant, 1996, 11 Suppl 1:, 41-3
Abstract: Between 1985 to 1992, 2545 renal transplantation (RTx) were performed as pre-emptive grafts in adults. This procedure represented 7.2% of first RTx for patients starting first renal replacement therapy (RRT) during this period, 6.1% of RTx performed in 1992 and 5.6% of all RTx ever performed and reported to the EDTA Registry. The procedure is more frequent in cases of live donor grafts, representing one third of pre-emptive RTx. Both 5 year patient and graft survivals are unaffected by dialysis duration prior to the first RTx: none in pre-emptive, < 1 years, 1-5 years or > 5 years. In our personal view, this procedure should be developed.
Language of Publication: English
Unique Identifier: 96328917

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MeSH Heading (Major): Kidney Transplantation|*
MeSH Heading: Adolescence; Adult; Aged; Graft Survival; Human; Middle Age

Publication Type: JOURNAL ARTICLE
ISSN: 0931-0509
Country of Publication: ENGLAND

Record 85 from database: MEDLINE
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Title: Absorption-enhancing mechanism of EDTA, caprate, and decanoylcarnitine in Caco-2 cells.
Author: Tomita M; Hayashi M; Awazu S
Address: Department of Biopharmaceutics, Tokyo University of Pharmacy and Life Science, Japan.
Source: J Pharm Sci, 1996 Jun, 85:6, 608-11
Abstract: The mechanism of paracellular expansion by absorption enhancers, e.g., EDTA, sodium caprate (C10), and decanoylcarnitine (DC), was studied, the focus being on the process of actin microfilament contraction in the tight junction. The effects of various inhibitors such as KN-62 (a specific inhibitor of Ca2+/calmodulin dependent protein kinase), H7 (a protein kinase C (PKC) inhibitor), and W7 (a calmodulin antagonist) were examined on the paracellular expansion by the enhancers in Caco-2 cells. From the experimental results, the following mechanisms were suggested. EDTA activates PKC by depletion of extracellular calcium via chelation resulting in expansion of the paracellular route. C10 increases the intracellular calcium level by an interaction with the cell membrane independent of cell polarity resulting in contraction with actin microfilament. DC interacts specifically with the apical membrane to increase the intracellular calcium level, but the mechanistic details subsequent to the increase of calcium are not clear.
Language of Publication: English
Unique Identifier: 96370018

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MeSH Heading (Major): Caco-2 Cells|*DE/*ME; Carnitine|*AA/PD; Decanoic Acids|*PD; Egtazic Acid|*PD; Intestinal Absorption|*DE
MeSH Heading: Calcium|ME/PD; Cell Membrane Permeability|DE; Dextrans|PK; Electric Impedance; Epithelium|DE/ME/PH; Fluorescein-5-isothiocyanate|AA/PK; Human; Intestinal Mucosa|DE/ME; Intracellular Fluid|ME; Support, Non-U.S. Gov't

Publication Type: JOURNAL ARTICLE
ISSN: 0022-3549
Country of Publication: UNITED STATES

Record 86 from database: MEDLINE
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Title: Repeated successful pregnancies after kidney transplantation in 102 women (Report by the EDTA Registry).
Author: Ehrich JH; Loirat C; Davison JM; Rizzoni G; Wittkop B; Selwood NH; Mallick NP
Address: Charite Children's Hospital, Humboldt University, Berlin, Germany.
Source: Nephrol Dial Transplant, 1996 Jul, 11:7, 1314-7
Abstract: BACKGROUND. Between 1967 and 1990, 820 successful pregnancies in 718 women on renal replacement therapy (RRT) were reported to the EDTA Registry. METHODS. This study analyses data on repeated successful pregnancies in 102 of these women, of whom 99 had two and three had three pregnancies. RESULTS. Primary renal diseases were mainly glomerulonephritis (41%), pyelonephritis (32%), and congenital malformations such as cystic diseases and hypoplasia or dysplasia (3%). Mean age at start of RRT was 21 years +/-5 SD. Ninety-four per cent of the women had the same transplant during the first and second pregnancies; 85% of these were alive with their first graft and 9% with a second graft; 4% were retransplanted after the first pregnancy and 2% were back on dialysis during the second pregnancy. Of the mothers with two successful pregnancies, two-thirds had a serum creatinine below 121 micromol/l after the first or after the second pregnancy. Six mothers lost their first graft after the first pregnancy. None of the mothers had died after delivery of the second or third baby. Several features of the first and the second pregnancy in these mothers were quite similar. Mean gestational age was 36 weeks+/-3SD during first and second pregnancy. Mean birth weight (height) of the first child was 2490 g+/-660 SD (48 cm+/-4 SD) and 2587 g+/-639 SD (50 cm+/-3 SD) of the second child (NS). Neonatal mortality was 4% after the first and second delivery; congenital abnormalities were found in five and three children respectively. CONCLUSIONS. Fourteen per cent of mothers who had a successful pregnancy on RRT subsequently had a second baby. Repeated pregnancies should not adversely affect graft function and/or fetal development provided that graft function was well preserved at the time of conception.
Language of Publication: English
Unique Identifier: 96325349

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MeSH Heading (Major): Kidney Diseases|*CO/*SU; Kidney Transplantation|*/AE/PH; Pregnancy Complications|*
MeSH Heading: Adult; Female; Human; Infant, Newborn; Pregnancy; Pregnancy Outcome; Registries

Publication Type: JOURNAL ARTICLE
ISSN: 0931-0509
Country of Publication: ENGLAND

Record 87 from database: MEDLINE
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Title: LPS induced release of IL-1 beta, IL-6, IL-8 and TNF-alpha in EDTA or heparin anticoagulated whole blood from persons with high or low levels of serum HDL.
Author: Eggesb‡ JB; Hjermann I; H‡stmark AT; Kierulf P
Address: Department of Clinical Chemistry, UllevÁal University Hospital, Oslo, Norway.
Source: Cytokine, 1996 Feb, 8:2, 152-60
Abstract: We have examined the lipopolysaccharide (LPS) induced release of interleukin 1 beta (IL-1 beta), IL-6, IL-8 and tumour necrosis factor alpha (TNF-alpha), in EDTA or heparin anticoagulated whole blood, from persons with high or low levels of high density lipoprotein (HDL), incubated at 37 degrees C for up to 20 h. In general, 100 ng LPS/ml gave two to twenty times higher responses than 1 ng LPS/ml, and heparin ten to hundred times higher than EDTA. The release of IL-8 was significantly higher in persons with high HDL, and was correlated with HDL, and inversely correlated with triglycerides and sCD14. The release of IL-1 beta, IL-6 and TNF-alpha was correlated with total cholesterol at medium doses of LPS (100 ng/ml), and inversely correlated with lipopolysaccharide-binding protein (LBP) at low doses of LPS (1 ng/ml). Serum levels of LBP were higher in persons with HDL, although not significantly. These results show LPS responsiveness of pro-inflammatory cytokines in whole blood from persons with high and low levels of HDL to be different, presumably of importance in inflammation and atherogenesis.
Language of Publication: English
Unique Identifier: 96232824

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MeSH Heading (Major): Anticoagulants|*PD; Interleukins|*BL; Lipopolysaccharides|*PD; Lipoproteins, LDL|*BL; Tumor Necrosis Factor|*ME
MeSH Heading: Antigens, CD14|BL; Edetic Acid|PD; Heparin|PD; Human; Interleukin-1|BL; Interleukin-6|BL; Interleukin-8|BL; Support, Non-U.S. Gov't

Publication Type: CLINICAL TRIAL; JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
ISSN: 1043-4666
Country of Publication: UNITED STATES

Record 88 from database: MEDLINE
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Title: Incidence and diagnosis of EDTA-dependent pseudothrombocytopenia in a consecutive outpatient population referred for isolated thrombocytopenia.
Author: Silvestri F; Virgolini L; Savignano C; Zaja F; Velisig M; Baccarani M
Address: Department of Medical and Morphological Research, Udine University School of Medicine, Italy.
Source: Vox Sang, 1995, 68:1, 35-9
Abstract: Among 111 patients referred to our outpatient clinic for isolated thrombocytopenia during a 24-month period, 17 (15.3%) cases of EDTA-dependent pseudothrombocytopenia (EDTA-PTCP) were identified. EDTA-PTCP represented the second most frequent cause of thrombocytopenia in this population. The diagnosis was confirmed by the following findings: (a) normal platelet numbers immediately after blood withdrawal; (b) progressive fall of platelet counts and evident platelet clumping over time, only in EDTA-anticoagulated blood. A simple, inexpensive and quick diagnostic method was devised, that consists in evaluating the platelet number in a blood sample anticoagulated with EDTA immediately after blood withdrawal and 4 h later.
Language of Publication: English
Unique Identifier: 95242697

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MeSH Heading (Major): Edetic Acid|*AE; Thrombocytopenia|CI/DI/*EP
MeSH Heading: Adult; Aged; Ambulatory Care Facilities; Comparative Study; Female; Human; Incidence; Male; Middle Age; Platelet Count|DE

Publication Type: JOURNAL ARTICLE
ISSN: 0042-9007
Country of Publication: SWITZERLAND

Record 89 from database: MEDLINE
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Title: Provocative chelation with DMSA and EDTA: evidence for differential access to lead storage sites.
Author: Lee BK; Schwartz BS; Stewart W; Ahn KD
Address: Institute of Industrial Medicine, Soonchunhyang University, Chunan, Republic of Korea.
Source: Occup Environ Med, 1995 Jan, 52:1, 13-9
Abstract: OBJECTIVES--To validate a provocative chelation test with 2,3-dimercaptosuccinic acid (DMSA) by direct comparison with the standard ethylene diamine tetraacetic acid (EDTA) test in the same subjects; and to compare and contrast the predictors of lead excretion after DMSA with those after EDTA. A metal chelating agent given orally, DMSA may mobilise and enhance the excretion of lead from the storage sites in the body that are most directly relevant to the health effects of lead. A provocative chelation test with DMSA could thus have wide potential application in clinical care and epidemiological studies. METHODS--34 male lead workers in the Republic of Korea were given a single oral dose of 10 mg/kg DMSA, urine was collected over the next eight to 24 hours, and urine volume and urinary lead concentration determined at 0, 2, 4, 6, 8, and 24 hours. Either two weeks before or two weeks after the dose of DMSA 17 of these workers also received 1 g intravenous EDTA followed by an eight hour urine collection with fractionation at 0, 2, 4, 6, and 8 hours. RESULTS--Urinary lead concentration peaked at two hours after DMSA and four hours after EDTA. Lead excretion after DMSA was less than after EDTA, and cumulative excretion after DMSA plateaued at six to eight hours. The two hour and four hour cumulative lead excretions after DMSA were highly correlated with the eight hour total (r = 0.76 and 0.95). In multiple linear regression analyses, blood lead was found to be an important predictor of EDTA-chelatable lead, whereas urinary aminolevulinic acid (ALAU) was associated with DMSA-chelatable lead. Notably, lead excretion after DMSA was greatly increased if EDTA was given first. An earlier dose of EDTA also modified the relation between ALAU and DMSA-chelatable lead in that workers who received EDTA before DMSA showed a much steeper dose-response relation between these two measures. CONCLUSIONS--The predictors of lead excretion after DMSA and EDTA are different and an earlier dose of EDTA may increase lead excretion after a subsequent dose of DMSA. The results suggest that two hour or four hour cumulative lead excretion after DMSA may provide an estimate of lead in storage sites that are most directly relevant to the health effects of lead.
Language of Publication: English
Unique Identifier: 95211216

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MeSH Heading (Major): Chelation Therapy|*; Edetic Acid|*TU; Lead|*; Occupational Exposure|*; Succimer|*TU
MeSH Heading: Adult; Comparative Study; Dose-Response Relationship, Drug; Environmental Monitoring; Human; Korea; Male; Middle Age; Time Factors

Publication Type: CLINICAL TRIAL; JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
ISSN: 1351-0711
Country of Publication: ENGLAND

Record 90 from database: MEDLINE
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Title: EDTA-dependent pseudothrombocytopenia. Association with antiplatelet and antiphospholipid antibodies.
Author: Bizzaro N; Brandalise M
Address: Laboratorio di Patologia Clinica, Ospedale Civile, S.DonÄa di Piave (Venice), Italy.
Source: Am J Clin Pathol, 1995 Jan, 103:1, 103-7
Abstract: In a study of 88 patients with EDTA-dependent pseudothrombocytopenia (PTCP), EDTA-dependent antiplatelet antibodies were seen in the sera of 72 (81.8%) patients (44 IgM, 25 IgG, and 3 IgA). The same sera also were tested for anticardiolipin antibodies (aCL), and 56 (63.6%) patients had sera that also were reactive for aCL (33 IgM, 21 IgG, and 2 IgA). The 16 patients who were negative for antiplatelet antibodies also were negative for aCL antibody. Overall concordance between antiplatelet and aCL antibodies was 82.9%; the correlation between antiplatelet and aCL antibody isotype distribution was 82.1%. Following cardiolipin absorption, most of the PTCP-sera were negative for antiplatelet activity, and no longer reproduced platelet clumping when incubated with normal blood. This finding showed that the antiplatelet antibodies cross-reacted with negatively charged phospholipids. However, after absorption on normal platelets, complete inhibition of aCL activity was observed in 34 (60.7%), and partial inhibition in 14 of the 56 patients who were aCL positive. These findings support the hypothesis that antibody subpopulations (naturally occurring autoantibodies) directed against negatively charged phospholipids can bind to antigens modified by EDTA on the platelet membrane, and may be responsible for PTCP genesis.
Language of Publication: English
Unique Identifier: 95117693

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MeSH Heading (Major): Antibodies, Antiphospholipid|*AN; Autoantibodies|*AN; Blood Platelets|*DE/*IM; Edetic Acid|*PD; Thrombocytopenia|*CI
MeSH Heading: Absorption; Adolescence; Adult; Aged; Aged, 80 and over; Cardiolipins|PD; Cells, Cultured; Child; Female; Human; Male; Middle Age; Platelet Aggregation

Publication Type: JOURNAL ARTICLE
ISSN: 0002-9173
Country of Publication: UNITED STATES

Record 91 from database: MEDLINE
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Title: Transverse relaxation of saline and plasma using Mn(II), HSA-EDTA-Mn, and HSA-EDTA-Gd: application to erythrocyte water exchange.
Author: Caines GH; Morgan CF; Long RC Jr; Goldstein JH
Address: Department of Chemistry, Emory University, Atlanta, Georgia 30322.
Source: Magn Reson Med, 1987 Sep, 5:3, 269-77
Abstract: Enhancements of 1H nuclear magnetic transverse relaxation rates by Mn(II)- and Gd(III)-ethylenediaminetetraacetic acid (EDTA)-human serum albumin (HSA) complexes are compared with MnCl2 in human plasma and isotonic saline. HSA-EDTA-Mn and HSA-EDTA-Gd in saline, at physiological concentrations of HSA, afforded T2's comparable to 2 mM MnCl2 in 4% HSA saline. The above reagents are new and potentially useful relaxation agents for the measurement of H2O exchange rates in erythrocyte suspensions. The exchange rate of water across the red cell membrane was determined using these new reagents over the temperature range from 20 to 37 degrees C.
Language of Publication: English
Unique Identifier: 88121377

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MeSH Heading (Major): Edetic Acid|*DU; Erythrocytes|*ME; Gadolinium|*DU; Manganese|*DU; Nuclear Magnetic Resonance|*; Serum Albumin|*DU; Water|*ME
MeSH Heading: Biological Transport; Comparative Study; Human; Indicators and Reagents; Ions; Isotonic Solutions; Plasma; Sodium Chloride; Support, U.S. Gov't, P.H.S.

Publication Type: JOURNAL ARTICLE
ISSN: 0740-3194
Country of Publication: UNITED STATES
CAS Registry/EC Number: 0 (serum albumin-EDTA-gadolinium complex); 0 (serum albumin-EDTA-manganese complex); 0 (Indicators and Reagents); 0 (Ions); 0 (Isotonic Solutions); 0 (Serum Albumin); 60-00-4 (Edetic Acid); 7439-96-5 (Manganese); 7440-54-2 (Gadolinium); 7647-14-5 (Sodium Chloride); 7732-18-5 (Water)

Record 92 from database: MEDLINE
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Title: Toxicological profile, current use, and regulatory issues on EDTA compounds for assessing use of sodium iron EDTA for food fortification.
Author: Whittaker P; Vanderveen JE; Dinovi MJ; Kuznesof PM; Dunkel VC
Address: Center for Food Safety and Applied Nutrition, Food and Drug Administration, Washington, DC 20204.
Source: Regul Toxicol Pharmacol, 1993 Dec, 18:3, 419-27
Abstract: The U.S. Food and Drug Administration (FDA) has approved the use of disodium and calcium disodium ethylenediaminetetraac etate (EDTA) for direct addition to food. The international nutrition community is interested in obtaining approval for the use of sodium iron(III) EDTA (NaFeEDTA) for dietary iron fortification because of its high iron bioavailability, its enhancement of intrinsic food iron uptake, and its stability under storage and food preparation conditions. A major concern in the United States has been the presumed extensive use of EDTA in the American food supply. Recently, an update of the estimated exposure to EDTA suggests that the exposure is much lower than previously assumed. This reduction may allow new uses of NaFeEDTA in food. The following issues are discussed in relation to the possible use of NaFeEDTA: toxicological profile of EDTA compounds, acceptable daily intake of EDTA, and estimated daily intake of EDTA in the United States.
Language of Publication: English
Unique Identifier: 94174038

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MeSH Heading (Major): Edetic Acid|AA/*AD/*TO
MeSH Heading: Ferric Compounds|AD; Food Analysis; Food, Fortified; Human; Statistics; United States; United States Food and Drug Administration

Publication Type: JOURNAL ARTICLE; REVIEW; REVIEW, TUTORIAL
ISSN: 0273-2300
Country of Publication: UNITED STATES
CAS Registry/EC Number: 0 (Ferric Compounds); 15275-07-7 (Fe(III)-EDTA); 60-00-4 (Edetic Acid)

Record 93 from database: MEDLINE
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Title: Agglutination of an EDTA blood sample caused by an EDTA-dependent panagglutinin.
Author: Reid ME; Bottenfield LK; Toy PT; Ellisor SS; Hart CA
Address
Source: Am J Clin Pathol, 1985 Apr, 83:4, 534-5
Abstract: A novel example of an EDTA-dependent panagglutinin is described. A blood sample drawn into EDTA for a routine hematologic workup demonstrated strong agglutination due to EDTA-dependent panagglutinins. Previous examples have been detected because of discrepant ABO results. This is the first report of an EDTA-dependent panagglutinin that caused agglutination in the EDTA sample collection tube and a false positive direct antiglobulin test.
Language of Publication: English
Unique Identifier: 85171844

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MeSH Heading (Major): Agglutinins|AN/*PH; Edetic Acid|*/PD; Hemagglutination|*/DE
MeSH Heading: Blood Grouping and Crossmatching; Case Report; Coombs' Test; Female; Human; Middle Age

Publication Type: JOURNAL ARTICLE
ISSN: 0002-9173
Country of Publication: UNITED STATES
CAS Registry/EC Number: 0 (Agglutinins); 60-00-4 (Edetic Acid)

Record 94 from database: MEDLINE
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Title: Combined treatment of medullary sponge kidney by EDTA potassium citrate and extracorporeal shock wave lithotripsy.
Author: Nakatsuka S; Kinoshita H; Ueda H; Araki T; Tanaka H
Address: Department of Urology, Kawasaki Medical School, Japan.
Source: Eur Urol, 1988, 14:4, 339-42
Abstract: A case of successful renal calculus dissolution by the combined treatment which consists of irrigation with ethylenediaminetetraacetic acid (EDTA), potassium citrate, and extracorporeal shock-wave lithotripsy (ESWL) is described here. Renal irrigation via nephrostomy, which was the main treatment, was attempted on a 34-year-old Japanese male who had bilateral nephrocalcinosis caused by type 1 renal tubular acidosis associated with an impacted calculus in the right ureter. Finally, most of the calculi have been dissolved within 1 year.
Language of Publication: English
Unique Identifier: 89005241

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MeSH Heading (Major): Citrates|*TU; Edetic Acid|*TU; Kidney Calculi|ET/*TH; Kidney, Sponge|CO/*TH; Lithotripsy|*; Nephrocalcinosis|ET/*TH
MeSH Heading: Adult; Case Report; Human; Irrigation; Male

Publication Type: JOURNAL ARTICLE
ISSN: 0302-2838
Country of Publication: SWITZERLAND
CAS Registry/EC Number: 0 (Citrates); 60-00-4 (Edetic Acid); 77-92-9 (Citric Acid)

Record 95 from database: MEDLINE
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Title: Evaluation of the EDTA-washed diet for use in the experimental production of zinc deficiency in human subjects.
Author: Cossack ZT; van den Hamer CJ
Address
Source: Int J Vitam Nutr Res, 1987, 57:1, 99-102
Abstract: Ethylenediaminetetraacetate (EDTA) is known to bind zinc (Zn) and other metals. EDTA-washed soy protein-based diet has been extensively used as a dietary model for the production of Zn-deficiency in human subjects as well as in experimental animals. The present study was conducted to test this diet for possible contamination with EDTA (inhereted by washing procedure) and also to evaluate it for its acceptability and palatibility by human subjects. In the first experiment, EDTA-washed soy protein-based diets were prepared as previously described (repeated washing with EDTA-sodium salt followed by washing with deionized water and filteration), but 14C-EDTA was used. Scintillation counter analysis showed no traces of 14C in the prepared diet (100% of the 14C activity was recovered in the filtrate). In the second experiment, the diets were prepared using inactive EDTA, then fed to 10 volunteers for sensory evaluation. Results of this trial showed that the palatibility and acceptability of these diets are generally very poor. In conclusion, the use of EDTA-washing procedure did not result in any contamination of the soy assay protein with the EDTA. However, the present dietary model may not be used to induce zinc deficiency in human volunteers on a long term basis since the palatibility of the washed product is poor.
Language of Publication: English
Unique Identifier: 87221316

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MeSH Heading (Major): Diet|*; Edetic Acid|*/AN; Zinc|*DF
MeSH Heading: Dietary Proteins|AN; Female; Food Contamination; Human; Male; Models, Biological; Vegetable Proteins

Publication Type: JOURNAL ARTICLE
ISSN: 0300-9831
Country of Publication: SWITZERLAND
CAS Registry/EC Number: 0 (Dietary Proteins); 0 (Soy Proteins); 0 (Vegetable Proteins); 60-00-4 (Edetic Acid); 7440-66-6 (Zinc)

Record 96 from database: MEDLINE
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Title: Injurious effect of EDTA contamination on colorimetry of serum iron.
Author: Koopman BJ; Hindriks FR; Lokerse YG; Wolthers BG; Orverdijk JF
Address
Source: Clin Chem, 1985 Dec, 31:12, 2030-2
Abstract: Colorimetry of iron in serum with Ferrozine (as used in the Technicon SMAC) or with bathophenanthroline (as used in the Du Pont aca) is influenced by EDTA, in contrast to such measurements with atomic absorption spectroscopy. Therefore EDTA contamination should be avoided with these colorimetric methods. If, however, contamination with EDTA is suspected, addition of zinc sulfate to serum or to the SMAC "ascorbic acid reagent" will cancel the influence of EDTA on measurements of iron in the SMAC.
Language of Publication: English
Unique Identifier: 86053961

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MeSH Heading (Major): Edetic Acid|*BL; Iron|*BL
MeSH Heading: Ascorbic Acid; Autoanalysis; Colorimetry; Human; Spectrophotometry, Atomic Absorption; Sulfates; Zinc

Publication Type: JOURNAL ARTICLE
ISSN: 0009-9147
Country of Publication: UNITED STATES
CAS Registry/EC Number: 0 (Sulfates); 50-81-7 (Ascorbic Acid); 60-00-4 (Edetic Acid); 7439-89-6 (Iron); 7440-66-6 (Zinc); 7733-02-0 (Zinc Sulfate)

Record 97 from database: MEDLINE
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Title: EDTA clearance in monitoring cisplatin dose escalation in patients with bulky metastatic germ cell tumors of the testis.
Author: Jeli0 S; Kovcin V; Babovi N; Vuleti L
Address: Institut za Onkologiju i Radiologiju, Beograd, Yugoslavia.
Source: Acta Oncol, 1988, 27:5, 541-3
Abstract: A fast cisplatin clearance may reduce exposure time of tumor cells to the drug, and thus impair the expected effects of dose escalation. This hypothesis was tested in 23 patients with bulky metastatic germ cell tumors of the testis, treated with etoposide, bleomycin and high-dose cisplatin (60 mg/m2/24 h x 4). The daily dose was retrospectively calculated in mg/l EDTA clearance/24 h. A daily dose of 60 mg/m2 of cisplatin in a person with a body surface of 1.7 m2 and EDTA clearance of 100 ml/min was equivalent to 0.69 mg cisplatin/l EDTA clearance/24 h. In the whole group, 10 patients had complete remission (CR), 10 partial response (PR) and 3 progressive disease (PD). The mean daily cisplatin dose (mean) in the whole group was 0.86 mg/l EDTA clearance/24 h (range 0.35-2.00). For patients with CR, mean +/- SD was 1.00 +/- 0.46, for those with PR 0.80 +/- 0.44, and for those with PD only 0.61 +/- 0.07. A cisplatin dose over 0.86 mg/l EDTA clearance/24 h x 4 was obtained in 6/10 patients with CR versus 2/13 patients with PR + PD. Patients with PD received a significantly lower cisplatin dose than the whole group (0.61 versus 0.86 mg cisplatin/l EDTA clearance/24 h x 4.) The difference between the average toxicity grade after cisplatin dose over and below 0.69 mg/l EDTA clearance/24 h x 4 was significant only for leukocytes (WHO grade 2.17 versus 1.36). Thus, the effective escalated dose of cisplatin should preferably be calculated not per m2 body surface but per 1 liter EDTA clearance. The 'ideal' escalated dose might be about 0.86-1.0 mg cisplatin/l EDTA clearance 24 h x 4.
Language of Publication: English
Unique Identifier: 89076620

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MeSH Heading (Major): Cisplatin|*AD/AE/PK; Edetic Acid|*PK; Neoplasms, Germ Cell and Embryonal|*DT; Testicular Neoplasms|*DT
MeSH Heading: Human; Male; Metabolic Clearance Rate; Monitoring, Physiologic; Neoplasm Metastasis

Publication Type: JOURNAL ARTICLE
ISSN: 0284-186X
Country of Publication: SWEDEN
CAS Registry/EC Number: 15663-27-1 (Cisplatin); 60-00-4 (Edetic Acid)

Record 98 from database: MEDLINE
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Title: Studies on EDTA extracts and collagenase digests from osteoporotic cancellous bone of the femoral head.
Author: Mbuyi-Muamba JM; Gevers G; Dequeker J
Address: Arthritis and Metabolic Bone Disease Research Unit, K. U. Leuven, Universitair Ziekenhuis, Pellenberg, Belgium.
Source: Clin Biochem, 1987 Jun, 20:3, 221-4
Abstract: Using EDTA extraction and collagenase digestion, cancellous bone of the femoral heads from 10 normal and 9 osteoporotic subjects were analyzed for their contents of collagen, sialoprotein, proteoglycan and carbohydrate. The percentage of extracted matrix proteins of the osteoporotic bone in EDTA was significantly decreased, as was the collagenase-resistant fraction (p less than 0.05). The sialic acid level in osteoporotic bone matrix was lower than in controls (p less than 0.05). The alterations found in bone matrix constituents in osteoporotic bone relative to controls suggest that in osteoporosis and fractures, not only bone mass changes, but also bone quality changes play a role in bone strength.
Language of Publication: English
Unique Identifier: 88002793

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MeSH Heading (Major): Bone and Bones|*AN; Microbial Collagenase|*AN; Tissue Extracts|*AN
MeSH Heading: Aged; Dialysis; Edetic Acid; Female; Femur Head|AN; Human; Hydrolysis; Male; Middle Age; Osteoporosis|ME

Publication Type: JOURNAL ARTICLE
ISSN: 0009-9120
Country of Publication: CANADA
CAS Registry/EC Number: EC 3.4.24.3 (Microbial Collagenase); 0 (Tissue Extracts); 60-00-4 (Edetic Acid)

Record 99 from database: MEDLINE
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Title: Determination of cyclic 3'-5'-adenosine monophosphate in plasma by RIA methods in the presence of EDTA.
Author: Penttilä IM; Heikkinen H; Herranen J; Mononen I; Castren O; Tuomisto L
Address
Source: J Clin Chem Clin Biochem, 1986 Oct, 24:10, 747-50
Abstract: Calcium ions definitively increase the ability of cyclic 3',5'-adenosine monophosphate (cAMP) to bind to its antibody. In contrast, ethylenedinitrolotetra-acetic acid as its disodium salt (EDTA) shows a dose-dependent inhibition of the binding of cAMP to its antibody. The less sensitive protein binding methods are not affected by EDTA. This is inconvenient, because the EDTA-plasmas can be stored frozen without breakdown of cAMP, but are unsuitable for sensitive radioimmunoassays. The aim of this investigation was to determine how calcium ions and EDTA affect the binding of cAMP to its antibody. Based on these results, we describe an alternative procedure for commercial RIA methods for the determination of cAMP in EDTA-plasma. The almost complete inhibition of the hapten-antibody reaction by EDTA can be abolished by adding an equivalent concentration of calcium ions to the reaction medium together with trichloroacetic acid. Thus a simple and rapid procedure was found for the storage of plasma and for the determination of plasma cAMP.
Language of Publication: English
Unique Identifier: 87059671

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MeSH Heading (Major): Cyclic AMP|*BL; Radioimmunoassay|*MT
MeSH Heading: Calcium; Edetic Acid; Female; Human; Pregnancy

Publication Type: JOURNAL ARTICLE
ISSN: 0340-076X
Country of Publication: GERMANY, WEST
CAS Registry/EC Number: 60-00-4 (Edetic Acid); 60-92-4 (Cyclic AMP); 7440-70-2 (Calcium)

Record 100 from database: MEDLINE
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Title: The blood tumour barrier in intracranial tumours studied with X-ray computed tomography and positron emission tomography using 68-Ga-EDTA.
Author: Mosskin M; von Holst H; Ericson K; Norén G
Address
Source: Neuroradiology, 1986, 28:3, 259-63
Abstract: Disruption of the blood brain barrier or rather blood tumour barrier in cerebral tumours was studied with CT after intravenous injection of contrast medium and with PET after intravenous administration of 68-Ga-EDTA. Histology from stereotactic biopsies or open surgery is compared with the radiologic findings and advantages of the respective methods are discussed. The material consisted of 47 patients mainly with supratentorial gliomas and a few miscellaneous tumours. Astrocytomas (Kernohan grade II) were found to have no disruption of blood tumour barrier while anaplastic astrocytomas and glioblastomas (Kernohan grade III and IV) had. PET is somewhat superior to CT in detection of disruption of the blood tumour barrier. It is concluded that the combination of CT and PET is of value in the assessment of intracranial tumours.
Language of Publication: English
Unique Identifier: 86257773

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MeSH Heading (Major): Astrocytoma|*PP; Blood-Brain Barrier|*; Brain Neoplasms|*PP; Glioblastoma|*PP; Meningeal Neoplasms|*PP; Meningioma|*PP
MeSH Heading: Adolescence; Adult; Aged; Child; Edetic Acid|DU; Female; Gallium Radioisotopes|DU; Human; Male; Middle Age; Support, Non-U.S. Gov't; Tomography, Emission-Computed; Tomography, X-Ray Computed

Publication Type: JOURNAL ARTICLE
ISSN: 0028-3940
Country of Publication: GERMANY, WEST
CAS Registry/EC Number: 0 (Gallium Radioisotopes); 60-00-4 (Edetic Acid)

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SUBSCRIBE: The Wednesday Letter is a free electronic monthly newsletter written and published by Karl Loren. You can view more than 50 back issues of this publication by clicking here. The Wednesday Letter subscription list is maintained on a secure server, no name is ever given or sold to anyone, and it is never used except for this Newsletter. It is automatically published on the Tuesday night just before the first Wednesday of every month. You can subscribe to this free monthly electronic letter by entering your eMail address and name below. You will then automatically receive a request for confirmation, sent to whatever address you have entered. If you do NOT receive this confirmation request, then you will not be subscribed. There may have been an error with your address and you should resubmit. The letter is never sent twice to the same address -- so you do not have to worry about a duplicate subscription. When you receive this confirmation request you must reply to it, or your subscription will not become active. No one can subscribe your name, and address, without you being notified, and if you get an unwanted notice of subscription you only need to DO NOTHING and the subscription will NOT be active.

REMOVAL: You can remove yourself from the subscription list in several different ways. Click here to read about this entire newsletter system. Every edition of The Wednesday Letter is delivered to your address with YOUR name and address in view on the letter, with a link that allows you to remove THAT name from the subscription list. If you try to send this removal message from an address different from the one you used to send in your original confirmation, then you will get a warning notice first, sent to the subscription address, asking you to confirm that you want to be removed from the list -- by replying to THAT request for confirmation, you will then be automatically removed. Thus, no one else can unsubscribe you, from some other computer, without your knowledge. But, if you send in the unsubscribe notice from the same machine used to receive the Letter, then the removal from the subscription list is automatic.

Personal Message: When you send a personal message to Karl Loren, you will receive a personal reply as per his instructions. Karl pledges that every personal message will get a personal answer. When you provide your mail address, we will send you free information including our free catalog and a cassette tape lecture by Karl Loren about heart disease, no charge, by mail, even if outside the US. You can select particular information you would like to receive, along with the free cassette tape and catalog.

You can reach Vibrant Life in many ways, including by mail to Vibrant Life, 2808 N. Naomi St., Burbank, CA 91504. Within the US and Canada, use the toll free number: (800) 523-4521, the local number: (818) 558-1799, the FAX: (818) 558-7299, eMail to kimberly@oralchelation.com or any one of the hundreds of message forms throughout the 50 web sites. Vibrant Life normally ships the same day we get an order. There are message forms on each of the 100,000+ pages on this and other sites where you can communicate with Vibrant Life. Check out our companion site, at: http://www.oralchelation.net where Karl's 2000 page book is published. Karl Loren is the author and webmaster for this BOOK, as well as for another web site about ORAL CHELATION. His personal philosophical articles are at PHILOSOPHY.

Copyright © May 20, 2008 6:24 AM by Karl Loren on behalf of Vibrant Life, ALL RIGHTS RESERVED. Permission is granted for non-commercial downloading, copying, distribution or redistribution on two conditions: One, that some form of copyright notice is included in every copy distributed or copied, showing the copyright belonging to Vibrant Life, Burbank, CA, at www.oralchelation.com . The second condition is that the material is not to be used for any purpose contrary to the purposes and objectives of this site. This permission does not extend to materials on this site which are copyrighted by others.