EDTA Scientific Study Data -- 100 Studies

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Table Of Contents

EDTA Scientific Study Data -- 100 Studies

Compiled by
Karl Loren

Results for your Medlines query:: Words in title only: EDTA; Published in 1977 through 1999; Only select references with abstracts available; Show references published in English only; Show references pertaining to humans

Documents: 1 to 100 of 309

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Number	Title	Comments
...1...	Sodium iron EDTA [NaFe(III)EDTA] as a food fortificant does not influence absorption and urinary excretion of manganese in healthy adults.
...2...	Sodium iron EDTA [NaFe(III)EDTA] as a food fortificant: the effect on the absorption and retention of zinc and calcium in women.
...3...	Platelet release reaction during EDTA-induced platelet agglutinations and inhibition of EDTA-induced platelet agglutination by anti-glycoprotein II b/III a complex monoclonal antibody.
...4...	A scanning electron microscopical study of normal and fluorotic enamel demineralized by EDTA.
...5...	A scanning electron microscopical study of normal and fluorotic enamel demineralized by EDTA.
...6...	Hydrogen cyanide poisoning: treatment with cobalt EDTA.
...7...	Effects of hyaluronidase, trypsin, and EDTA on surface composition and topography during detachment of cells in culture.
...8...	EDTA soluble protein of human mature normal enamel.
...9...	Complement components detected on normal red blood cells taken into EDTA and CPD.
...10...	A convenient method of DNA extraction from blood anticoagulated with EDTA.
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...11...	Fe(III)-EDTA complex as iron fortification. Further studies.
...12...	Morphological and cytochemical study of Chlamydia with EDTA regressive technique and Gautier staining in ultrathin frozen sections of infected cell cultures: a comparison with embedded material.
...13...	[51Cr]EDTA plasma clearance and endogenous creatinine clearance in advanced renal insufficiency.
...14...	Effects of Ca, Mg, and EDTA on creatine kinase activity in cerebrospinal fluid.
...15...	Compensation of dietary induced reduction of tetracycline absorption by simultaneous administration of EDTA.
...16...	Isolation and characterization of outer and inner membranes from Pseudomonas aeruginosa and effect of EDTA on the membranes.
...17...	Single injection (51Cr)EDTA plasma clearance determination in children using capillary blood samples.
...18...	Precision of single injection (51Cr)EDTA plasma clearance and endogenous creatinine clearance determinations in children.
...19...	IgG platelet antibodies in EDTA-dependent pseudothrombocytopenia bind to platelet membrane glycoprotein IIb.
...20...	Metrizamide in urography. II. A comparison of 51Cr-EDTA clearance and metrizamide clearance in man.
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...21...	Fe(III)-EDTA complex as iron fortification.
...22...	Restoration of hemoglobin function in stored EDTA blood. Application in identification of hemoglobin variants with abnormal oxygen affinity.
...23...	GFR measurement with iohexol and 51Cr-EDTA. A comparison of the two favoured GFR markers in Europe.
...24...	Tissue inhibitor of metalloproteinase-2 protection of matrix metalloproteinase-2 from degradation by plasmin is reversed by divalent cation chelator EDTA and the bisphosphonate alendronate.
...25...	Effects of smoking on the urine excretion of oral 51Cr EDTA in ulcerative colitis.
...26...	Ultrasonic subgingival root planing and EDTA etching in a one-step procedure.
...27...	Lead exposure and accumulation in healthy Thais: assessed by lead levels, EDTA mobilization and heme synthesis-related parameters.
...28...	Defining the orientation of the human U1A RBD1 on its UTR by tethered-EDTA(Fe) cleavage.
...29...	Essential monoclonal gammopathy with an IgM paraprotein that is a cryoglobulin with cold agglutinin and EDTA-dependent platelet antibody properties.
...30...	The role of EDTA in provoking allergic reactions to subcutaneous infusion of apomorphine in patients with Parkinson's disease: a histologic study.
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...31...	EDTA chelation therapy should be more commonly used in the treatment of vascular disease.	EDTA chelation therapy is safe, effective, and more economical than commonly used surgical treatments for vascular disease. This article includes evidence of effectiveness, mechanisms of action of EDTA, a discussion of studies that have been done regarding the therapy, and some brief case reports. The conclusion is that EDTA chelation therapy should be a therapeutic option for vascular disease, either by itself or in conjunction with standard protocols.
...32...	Fibrinogen present in EDTA--anticoagulated plasma stimulates the tissue-type plasminogen activator-catalysed conversion of plasminogen to plasmin.
...33...	Freeze-dried fibrinogen or fibrinogen in EDTA stimulate the tissue-type plasminogen activator-catalysed conversion of plasminogen to plasmin.
...34...	An EDTA-associated anti-B agglutinin: the role of ionized calcium.
...35...	Aminoglycosides prevent and dissociate the aggregation of platelets in patients with EDTA-dependent pseudothrombocytopenia.
...36...	Iron release from recombinant N-lobe and single point Asp63 mutants of human transferrin by EDTA.
...37...	Assessment of glomerular filtration rate utilizing subcutaneously injected 51Cr-EDTA.
...38...	The analysis of EDTA in dried bloodstains by electrospray LC-MS-MS and ion chromatography.
...39...	Human nasal absorption of 51Cr-EDTA in smokers and control subjects.
...40...	Screening for EDTA-dependent deviations in platelet counts and abnormalities in platelet distribution histograms in pseudothrombocytopenia.
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...41...	Comparison of manual and automated cell counts in EDTA preserved synovial fluids. Storage has little influence on the results.
...42...	Comparison of EDTA and acid-citrate-dextrose collection tubes for detection of cytomegalovirus antigenemia and infectivity in leukocytes before and after storage.
...43...	Synergistic effect of gramicidin and EDTA in inhibiting sperm motility and cervical mucus penetration in vitro.
...44...	End-stage renal failure due to analgesic nephropathy, its changing pattern and cardiovascular mortality. EDTA-ERA Registry Committee.
...45...	Comparison of heparin and EDTA transport tubes for detection of cytomegalovirus in leukocytes by shell vial assay, pp65 antigenemia assay, and PCR.
...46...	A new type of pseudothrombocytopenia: EDTA-mediated agglutination of platelets bearing Fab fragments of a chimaeric antibody.
...47...	EDTA-plasma vs serum differences in cholesterol, high-density-lipoprotein cholesterol, and triglyceride as measured by several methods.	We conclude that P/S ratios vary by analytical methods, and that HDLC ratios tend to be larger in magnitude and in the opposite direction from TC and TG. Both effects lead to significant biases in computed disease risk.
...48...	Random locomotion and chemotaxis of human blood polymorphonuclear leukocytes (PMN) in the presence of EDTA: PMN in close quarters require neither leukocyte integrins nor external divalent cations.
...49...	Long-term precision of glomerular filtration rate measurements using 51Cr-EDTA plasma clearance.
...50...	Technetium (99mTc)-labelled white cell scanning, 51Cr-EDTA and 14C-mannitol-labelled intestinal permeability studies: non-invasive methods of diagnosing acute intestinal graft-versus-host disease [see comments]
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...51...	EDTA dependent pseudothrombocytopenia caused by antibodies against the cytoadhesive receptor of platelet gpIIB-IIIA.
...52...	Dynamic positron emission tomography for study of cerebral hemodynamics in a cross section of the head using positron-emitting 68Ga-EDTA and 77Kr.
...53...	Sequential preparation of highly purified microvillous and basal syncytiotrophoblast membranes in substantial yield from a single term human placenta: inhibition of microvillous alkaline phosphatase activity by EDTA.
...54...	Simultaneous urography and determination of glomerular filtration rate. A comparison of total plasma clearances of iohexol and 51Cr-EDTA in plegic patients.
...55...	Oxidation of human insulin-like growth factor I in formulation studies. 3. Factorial experiments of the effects of ferric ions, EDTA, and visible light on methionine oxidation and covalent aggregation in aqueous solution.
...56...	In vivo inhibition of serum dopamine-beta-hydroxylase by CaNa2 EDTA injection.
...57...	Effect of different concentrations of EDTA on smear removal and collagen exposure in periodontitis-affected root surfaces.
...58...	Inhibition of paraoxonase activity in human liver microsomes by exposure to EDTA, metals and mercurials.
...59...	90Y-labeled antibody uptake by human tumor xenografts and the effect of systemic administration of EDTA.
...60...	Quantitative capillary electrophoresis/ion spray tandem mass spectrometry determination of EDTA in human plasma and urine.
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...61...	EDTA and the absorption of iron from food.
...62...	Back pain following epidural anesthesia with 2-chloroprocaine (EDTA-free) or lidocaine [see comments]
...63...	Clinical evaluation of the efficacy of EDTA solution as an endodontic irrigant.	These results suggest that 15% EDTA solution is more effective than saline solution as a root canal irrigant.
...64...	Renal and extrarenal clearance of 99mTc-MAG3: a comparison with 125I-OIH and 51Cr-EDTA in patients representing all levels of glomerular filtration rate.
...65...	Analysis of 15 cases with platelet EDTA-dependent antibodies.
...66...	A novel method for isolation of Chlamydia pneumoniae by treatment with trypsin or EDTA.
...67...	Reproducibility of simplified techniques for the measurement of 51Cr-EDTA clearance.
...68...	How good is the slope of the second exponential for estimating 51Cr-EDTA renal clearance?
...69...	The cytotoxic interaction of inorganic trace elements with EDTA and cisplatin in sensitive and resistant human ovarian cancer cells.
...70...	Removing IgG antibodies from intact red cells: comparison of acid and EDTA, heat, and chloroquine elution methods.
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...71...	Flow cytometric evaluation of platelet activation in blood collected into EDTA vs. Diatube-H, a sodium citrate solution supplemented with theophylline, adenosine, and dipyridamole [see comments]
...72...	Report on management of renale failure in Europe, XXVI, 1995. Rare diseases in renal replacement therapy in the ERA-EDTA Registry.
...73...	Metal excretion and magnesium retention in patients with intermittent claudication treated with intravenous disodium EDTA.	Sixty patients with intermittent claudication participated in a double-blind placebo-controlled trial of 20 courses of intravenous chelation therapy with 3 g of disodium EDTA vs placebo during 5-9 weeks. After the first infusion, the 24-h urinary excretion of lead and zinc was approximately 25-fold higher in the EDTA-treated group; relative differences for copper and calcium were smaller. Urinary magnesium excretion in the EDTA-treated group was one-third less than in the control group. After the treatment period, the blood lead concentration had decreased by approximately 73% and the serum zinc concentration by approximately 34%; other changes in blood concentrations were negligible. The loss of essential minerals and the possible redistribution of lead in the body may constitute a disadvantage that should be taken into account in repeated intravenous EDTA treatment.
...74...	Smear removal and collagen exposure after non-surgical root planing followed by etching with an EDTA gel preparation.
...75...	EDTA-dependent pseudothrombocytopenia: a clinical and epidemiological study of 112 cases, with 10-year follow-up.
...76...	Measurement of glomerular filtration rate by single-injection, single-sample techniques, using 51Cr-EDTA or iohexol.
...77...	More on false thrombocytopenias: EDTA-dependent pseudothrombocytopenia associated with a congenital platelet release defect.
...78...	C-reactive protein: the difference between quantitation is serum and EDTA plasma.
...79...	Production and characterization of monoclonal antibodies to the EDTA extract of Leptospira interrogans, serovar icterohaemorrhagiae.
...80...	EDTA chelation therapy in the treatment of vascular disease [see comments]	Ethylenediamine tetraacetic acid (EDTA) chelation therapy has been used for decades for the treatment of vascular disease, alone or in combination with other treatments. This article includes a historic review of the research literature, current evidence of effectiveness, potential mechanisms of action of EDTA, and some brief case reports. The authors conclude that EDTA chelation therapy is a valuable therapeutic option for vascular disease, either alone or in conjunction with standard treatment protocols.
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...81...	Plasma clearance rate of 51Cr-EDTA provides a precise and convenient technique for measurement of glomerular filtration rate in diabetic humans.
...82...	Survival modelling in kidney transplantation: hazard rates of graft loss. Transplant Working Group of the Registry Committee of the European Dialysis and Transplantation Association-European Renal Association (EDTA-ERA).
...83...	Malignancies after renal transplantation: the EDTA-ERA registry experience. European Dialysis and Transplantation Association-European Renal Association.
...84...	Transplantation Report. 2: Pre-emptive renal transplantation in adults aged over 15 years. The EDTA-ERA Registry. European Dialysis and Transplant Association-European Renal Association.
...85...	Absorption-enhancing mechanism of EDTA, caprate, and decanoylcarnitine in Caco-2 cells.
...86...	Repeated successful pregnancies after kidney transplantation in 102 women (Report by the EDTA Registry).
...87...	LPS induced release of IL-1 beta, IL-6, IL-8 and TNF-alpha in EDTA or heparin anticoagulated whole blood from persons with high or low levels of serum HDL.
...88...	Incidence and diagnosis of EDTA-dependent pseudothrombocytopenia in a consecutive outpatient population referred for isolated thrombocytopenia.
...89...	Provocative chelation with DMSA and EDTA: evidence for differential access to lead storage sites.	Both intravenously administered EDTA and an orally administered alternative proved to be effective in removing lead from the body.
...90...	EDTA-dependent pseudothrombocytopenia. Association with antiplatelet and antiphospholipid antibodies.
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...91...	Transverse relaxation of saline and plasma using Mn(II), HSA-EDTA-Mn, and HSA-EDTA-Gd: application to erythrocyte water exchange.
...92...	Toxicological profile, current use, and regulatory issues on EDTA compounds for assessing use of sodium iron EDTA for food fortification.
...93...	Agglutination of an EDTA blood sample caused by an EDTA-dependent panagglutinin.
...94...	Combined treatment of medullary sponge kidney by EDTA potassium citrate and extracorporeal shock wave lithotripsy.
...95...	Evaluation of the EDTA-washed diet for use in the experimental production of zinc deficiency in human subjects.	Ethylenediaminetetraacetate (EDTA) is known to bind zinc (Zn) and other metals. [It will bind these metals IN THE GUT as well as in the blood. Thus, when the EDTA is taken orally, it WILL bind metals, thus its use here. There is no inference that this EDTA was to bind metals in the blood.] EDTA-washed soy protein-based diet has been extensively used as a dietary model for the production of Zn-deficiency in human subjects as well as in experimental animals.
...96...	Injurious effect of EDTA contamination on colorimetry of serum iron.
...97...	EDTA clearance in monitoring cisplatin dose escalation in patients with bulky metastatic germ cell tumors of the testis.
...98...	Studies on EDTA extracts and collagenase digests from osteoporotic cancellous bone of the femoral head.
...99...	Determination of cyclic 3'-5'-adenosine monophosphate in plasma by RIA methods in the presence of EDTA.
...100..	The blood tumour barrier in intracranial tumours studied with X-ray computed tomography and positron emission tomography using 68-Ga-EDTA.

HealthGate Documents

Record 1 from database: MEDLINE
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Title: Sodium iron EDTA [NaFe(III)EDTA] as a food fortificant does not influence absorption and urinary excretion of manganese in healthy adults.
Author: Davidsson L; Almgren A; Hurrell RF
Address: Laboratory for Human Nutrition, Swiss Federal Institute of Technology, ETH ZÂurich, CH-8803 RÂuschlikon, Switzerland.
Source: J Nutr, 1998 Jul, 128:7, 1139-43
Abstract: NaFe(III)EDTA is a promising iron (Fe) compound for food fortification programs because of its high Fe bioavailability from meals containing dietary inhibitors of Fe absorption such as phytic acid. However, this Fe compound is not currently used in any large-scale fortification program because of concern over its possible negative influence on the metabolism of other essential minerals or its possible influence on the absorption of potentially toxic elements, such as manganese (Mn). In this study, Mn absorption and urinary excretion were studied in adults after intake of an Fe-fortified weaning cereal labeled with 54Mn. In a crossover design, the fortification of the weaning cereal with Fe as NaFeEDTA was compared with ferrous sulfate. Manganese absorption was measured by extrapolation from whole-body retention data 10-30 d after intake, and urinary excretion of 54Mn was measured over 7 d. No significant differences in 54Mn absorption or urinary excretion were found; 1.1 +/- 0.15 and 0.91 +/- 0.35% of the ingested dose was absorbed from the cereal fortified with NaFe(III)EDTA and FeSO4, respectively. Urinary excretion of 54Mn was very low; the total radioactivity in urine represented 1.1 +/- 0.55% of the absorbed dose with NaFe(III)EDTA and 0.72 +/- 0.53% of the absorbed dose with FeSO4. Until now, Fe-fortification programs have met with only limited success. The introduction of NaFeEDTA as a food fortificant could be a useful tool to provide bioavailable Fe to vulnerable groups in the population and thus aid in combating Fe deficiency.
Language of Publication: English
Unique Identifier: 98315238

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MeSH Heading (Major): Ferric Compounds|AD/*AE; Food, Fortified|*; Iron Chelating Agents|*; Manganese|AN/*PK/UR
MeSH Heading: Absorption; Adult; Cereals|CH; Cross-Over Studies; Edetic Acid|AD/AE; Female; Ferrous Compounds|AD; Human; Infant Food|AN; Iron|AN; Male; Middle Age; Nutritional Status; Phytic Acid|AN; Radioisotopes; Weaning

Publication Type: JOURNAL ARTICLE
ISSN: 0022-3166
Country of Publication: UNITED STATES

Record 2 from database: MEDLINE
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Title: Sodium iron EDTA [NaFe(III)EDTA] as a food fortificant: the effect on the absorption and retention of zinc and calcium in women.
Author: Davidsson L; Kastenmayer P; Hurrell RF
Address: NestlÆe Research Centre, Nestec Ltd, Lausanne, Switzerland.
Source: Am J Clin Nutr, 1994 Aug, 60:2, 231-7
Abstract: The iron fortificant NaFeEDTA could have a potential negative effect on the metabolism of other minerals. We have used stable isotopes to monitor zinc and calcium metabolism in 10 women consuming a single meal of high-extraction wheat rolls (100 g flour) fortified with 5 mg Fe as either FeSO4 or NaFeEDTA. Six-day chemical balances were made simultaneously to study apparent zinc and calcium retention from the complete diet containing the differently iron-fortified breads (200 g flour; 10 mg added Fe/d). Mean 70Zn absorption from the bread meal increased from 20.9% with FeSO4 to 33.5% with NaFeEDTA (P < 0.05) whereas mean 44Ca absorption was 53.3% from both breads. When NaFeEDTA-fortified bread was consumed, there was a small but significant increase in urinary excretion of 70Zn and 44Ca. There was a similar small increase in urinary zinc excretion during the 6-d balance, although the apparent retention of zinc and calcium was not different. Thus, we found no negative overall effect of NaFeEDTA consumption on the metabolism of zinc and calcium. In contrast, the results suggest that NaFeEDTA added to low-bioavailability diets might increase zinc absorption as well as provide iron with high bioavailability.
Language of Publication: English
Unique Identifier: 94303631

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MeSH Heading (Major): Calcium, Dietary|AD/*PK; Edetic Acid|*AD; Ferric Compounds|*AD; Food, Fortified|*; Iron Chelating Agents|*AD; Zinc|AD/*PK
MeSH Heading: Absorption; Adult; Biological Availability; Bread; Comparative Study; Diet; Female; Ferrous Compounds|AD; Human; Iron|BL; Middle Age

Publication Type: CLINICAL TRIAL; JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
ISSN: 0002-9165
Country of Publication: UNITED STATES

Record 3 from database: MEDLINE
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Title: Platelet release reaction during EDTA-induced platelet agglutinations and inhibition of EDTA-induced platelet agglutination by anti-glycoprotein II b/III a complex monoclonal antibody.
Author: Ryo R; Sugano W; Goto M; Takada M; Saigo K; Hashimoto M; Yamaguchi N
Address: Blood Transfusion Service, Kobe University Hospital, Japan.
Source: Thromb Res, 1994 May, 74:3, 265-72
Abstract: To characterize the nature of EDTA-induced platelet agglutination, the spontaneous release of beta-thromboglobulin (beta-TG) and platelet factor 4 (PF4) was examined during EDTA-induced platelet agglutinations. A slight release of beta-TG and PF4 was observed when EDTA-anticoagulated whole blood from cases with EDTA-induced platelet agglutination was kept for 60 minutes, whereas a high spontaneous release of these proteins was found from normal blood anticoagulated with EDTA. These findings imply that EDTA-dependent platelet agglutinin may stabilize the platelet membrane surfaces. Secondly, we found that pretreatment of fresh blood with anti-glycoprotein (GP) II b/III a complex monoclonal antibody dramatically reduced EDTA-induced platelet agglutinations. This study indicated that the binding sites of EDTA-dependent antibody might be GP II b/III a complex. The use of an anti-GP II b/III a complex monoclonal antibody may be useful in avoiding analytical errors in some cases with EDTA-induced pseudothrombocytopenia.
Language of Publication: English
Unique Identifier: 94317160

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MeSH Heading (Major): beta-Thromboglobulin|*ME; Blood Platelets|*ME; Platelet Factor 4|*ME; Platelet Membrane Glycoproteins|*IM
MeSH Heading: Adenosine Diphosphate; Antibodies, Monoclonal; Edetic Acid; Hemagglutination|DE; Human

Publication Type: JOURNAL ARTICLE
ISSN: 0049-3848
Country of Publication: UNITED STATES

Record 4 from database: MEDLINE
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Title: A scanning electron microscopical study of normal and fluorotic enamel demineralized by EDTA.
Author: Thylstrup A
Address
Source: Acta Odontol Scand, 1979, 37:3, 127-35
Abstract: Normal and fluorotic primary and permanent teeth were demineralized in 10% EDTA at pH 7 for varying periods of time up to 4 weeks. The fluorotic teeth initially dissolved at a much slower rate than non-fluorotic specimens and appeared to contain more organic material. After almost complete removal of the outer enamel a soft organic layer remained on the dentin surface. This consisted of a fine fibrillar mesh and some more dense material. After four weeks much of the organic material had disappeared leaving only a thin membrane on the dentin surface.
Language of Publication: English
Unique Identifier: 80016719

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MeSH Heading (Major): Dental Enamel|ME/*PA/UL; Dental Enamel Proteins|*ME; Edetic Acid|*PD; Fluorosis, Dental|ME/*PA
MeSH Heading: Human; Microscopy, Electron, Scanning; Minerals|ME

Publication Type: JOURNAL ARTICLE
ISSN: 0001-6357
Country of Publication: FINLAND

Record 5 from database: MEDLINE
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Title: A scanning electron microscopical study of normal and fluorotic enamel demineralized by EDTA.
Author: Thylstrup A
Address
Source: Acta Odontol Scand, 1979, 37:2, 127-35
Abstract: Normal and fluorotic primary and permanent teeth were demineralized in 10% EDTA at pH 7 for varying periods of time up to 4 weeks. The fluorotic teeth initially dissolved at a much slower rate than non-fluorotic specimens and appeared to contain more organic material. After almost complete removal of the outer enamel a soft organic layer remained on the dentin surface. This consisted of a fine fibrillar mesh and some more dense material. After four weeks much of the organic material had disappeared leaving only a thin membrane on the dentin surface.
Language of Publication: English
Unique Identifier: 79184364

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MeSH Heading (Major): Dental Enamel|DE/*UL; Dental Enamel Proteins|*ME; Edetic Acid|*PD; Fluorosis, Dental|ME/*PA
MeSH Heading: Human; Microscopy, Electron, Scanning; Minerals|ME

Publication Type: JOURNAL ARTICLE
ISSN: 0001-6357
Country of Publication: FINLAND

Record 6 from database: MEDLINE
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Title: Hydrogen cyanide poisoning: treatment with cobalt EDTA.
Author: Nagler J; Provoost RA; Parizel G
Address
Source: J Occup Med, 1978 Jun, 20:6, 414-6
Abstract: Three case reports are presented of employees who suffered varying degrees of exposure to hydrogen cyanide and their subsequent clinical courses following treatment with cobalt EDTA. A review of treatment modalities for CN- toxicity is given. It is concluded that, because of the degree of patient symptomatology associated from the use of cobalt EDTA, this therapy be reserved only for patients with the most severe degress of exposure to CN(-), and that in all other cases combined sodium nitrite and sodium thiosulphate therapy should be employed.
Language of Publication: English
Unique Identifier: 78219996

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MeSH Heading (Major): Cobalt|*TU; Edetic Acid|AE/*TU; Hydrogen Cyanide|*PO; Occupational Diseases|*CI/DT
MeSH Heading: Accidents, Occupational; Adult; Case Report; Environmental Exposure; Human; Male; Middle Age; Nitrites|TU; Sodium|TU; Thiosulfates|TU

Publication Type: JOURNAL ARTICLE
ISSN: 0096-1736
Country of Publication: UNITED STATES

Record 7 from database: MEDLINE
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Title: Effects of hyaluronidase, trypsin, and EDTA on surface composition and topography during detachment of cells in culture.
Author: Vogel KG
Address: Department of Anatomy, University of New Mexico, School of Medicine, Albuquerque 87131.
Source: Exp Cell Res, 1978 May, 113:2, 345-57
Abstract: Cultured human embryo fibroblasts (HLM18) were labeled with [3H]glucosamine and Na35SO4, and then treated with testicular hyaluronidase, trypsin, or EDTA. Macromolecular material from the surface of these cells was characterized by DEAE-cellulose chromatography and cetylpyridinium chloride precipitation while the associated morphology of cell detachment was studied by phase contrast and scanning electron microscopy. Release of surface glycosaminoglycans by testicular hyaluronidase did not cause cell rounding or detachment. EDTA did not release cell-surface components, but caused cell contraction and detachment morphologically similar to that caused by trypsin. Large amounts of cell-surface glycoproteins and glycosaminoglycans were released by trypsin. From these observations it is concluded that hyaluronic acid is not a principal adhesive agent in the attachment of cells to a substrate. It is suggested that both EDTA and trypsin may have their primary effect upon the cytoskeleton.
Language of Publication: English
Unique Identifier: 88329276

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MeSH Heading (Major): Cell Membrane|*DE/PH/UL; Edetic Acid|*PD; Hyaluronidase|*PD; Trypsin|*PD
MeSH Heading: Cell Adhesion|DE; Cell Line; Cytoskeleton|DE/PH; Glycosaminoglycans|PH; Human; Support, U.S. Gov't, P.H.S.

Publication Type: JOURNAL ARTICLE
ISSN: 0014-4827
Country of Publication: UNITED STATES
CAS Registry/EC Number: EC 3.2.1.35 (Hyaluronidase); EC 3.4.21.4 (Trypsin); 0 (Glycosaminoglycans); 60-00-4 (Edetic Acid)

Record 8 from database: MEDLINE
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Title: EDTA soluble protein of human mature normal enamel.
Author: Belcourt A; Gillmeth S
Address
Source: Calcif Tissue Int, 1979 Nov 6, 28:3, 227-31
Abstract: Pure human mature enamel was prepared using a careful microdissection technique. After EDTA dissolution, the soluble proteins were recovered representing a concentration of 0.035% in the initial enamel. When the samples were analyzed with polyacrylamide gel electrophoresis, Coomassie Brilliant Blue staining revealed only one sharp fast migrating band, whereas o-toluidine blue, methylene blue, Amido Black 10B, and pyronine red G showed a thin double band at the same migration distance. Ultracentrifugation studies suggested that the proteins were of low molecular weight or of weak density. Absorption spectra showed a strong absorbance at 260 nm. After hydrolysis, amino acid analyses yielded a composition of 25% Gly, 13.5% Glu, 11% Ser, and 11% Pro. Cysteine measured as cysteic acid was present at 2%, and 2% hydroxyproline was found. A carbohydrate content of 15% was estimated by the anthrone method. Glucose, galactose, mannose, and fucose, identified through gas chromatography, were in a molar ratio of 9:4:3:1. Thus the organic matrix of adult human enamel consists of one or possibly two acidic glycoproteins.
Language of Publication: English
Unique Identifier: 80066760

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MeSH Heading (Major): Dental Enamel Proteins|*AN
MeSH Heading: Amino Acids|AN; Carbohydrates|AN; Edetic Acid; Electrophoresis, Polyacrylamide Gel; Glycoproteins|AN; Human; Molecular Weight; Solubility

Publication Type: JOURNAL ARTICLE
ISSN: 0171-967X
Country of Publication: UNITED STATES

Record 9 from database: MEDLINE
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Title: Complement components detected on normal red blood cells taken into EDTA and CPD.
Author: Freedman J; Massey A
Address
Source: Vox Sang, 1979, 37:1, 1-8
Abstract: Normal red blood cells (RBC) from fresh EDTA and CPD blood and from stored CPD blood were examined for the presence of bound subcomponents of C3 and C4. By serologic agglutination tests, only C3d was detectable on the cells. Incubation in compatible fresh normal serum (FNS) at 37 degrees C appeared to increase the amount of 3Cd on the RBC. C3b was serologically detectable only on stored CPD cells and only after incubation in compatible FNS. No. C4 components were detected on the cell surfaces in agglutination tests. Using an indirect labeling technique, small, but significant, amounts of C3d and C4d were found on all three types of untreated cells. C3b was present on stored CPD cells only. The indirect labeling technique showed a significant increase in C3d and C4d on all cells following incubation i- compatible FNS, whereas bound C3b was significantly increased only with stored CPD cells. There was no increase in bound C4b following serum incubation. The average number of C3d molecules per cell on normal EDTA cells was 557 and average Ko was 3.6 x 10(7) l/mol.
Language of Publication: English
Unique Identifier: 80037735

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MeSH Heading (Major): Citrates|*PD; Complement 3|*IM; Complement 4|*IM; Edetic Acid|*PD; Erythrocytes|*IM
MeSH Heading: Animal; Binding, Competitive; Coombs' Test; Haplorhini; Hemagglutination Tests; Human; Immune Sera|PD; Iodine Radioisotopes; Rabbits

Publication Type: JOURNAL ARTICLE
ISSN: 0042-9007
Country of Publication: SWITZERLAND

Record 10 from database: MEDLINE
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Title: A convenient method of DNA extraction from blood anticoagulated with EDTA.
Author: Yokota M; Sindo K; Hiyoshi M; Tsuda I; Tatsumi N
Address: Department of Clinical and Laboratory Medicine, Osaka City University Medical School, Japan.
Source: Biochem Mol Biol Int, 1998 Jul, 45:3, 617-22
Abstract: Surplus blood often remains after routine clinical tests using EDTA-anticoagulated samples. To use this blood for DNA analysis, we isolated white cells by adherence to polyethylene terephthalate fibers, which could be stored for several weeks transported by mail after methanol fixation. DNA yield was sufficient and correlated with white cell count. Extracted DNA was free of hemoglobin contamination and durable to polymerase chain reaction and enzyme digestion, which yielded products visualized as well-separated bands on electrophoresis. We found our method to be practical for the routine clinical laboratory.
Language of Publication: English
Unique Identifier: 98344606

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MeSH Heading (Major): Cell Separation|*MT; DNA|*BL/IP; Leukocytes|*
MeSH Heading: Adult; Anticoagulants|PD; Edetic Acid|PD; Filtration|MT; Gene Amplification; Genotype; Human; HLA-DQ Antigens|CL/GE; Methanol; Polyethylene Terephthalates; Polymerase Chain Reaction

Publication Type: JOURNAL ARTICLE
ISSN: 1039-9712
Country of Publication: AUSTRALIA
CAS Registry/EC Number: 0 (Anticoagulants); 0 (HLA-DQ Antigens); 0 (HLA-DQA1); 0 (Polyethylene Terephthalates); 60-00-4 (Edetic Acid); 67-56-1 (Methanol); 9007-49-2 (DNA)

Record 11 from database: MEDLINE
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Title: Fe(III)-EDTA complex as iron fortification. Further studies.
Author: Martínez-Torres C; Romano EL; Renzi M; Layrisse M
Address
Source: Am J Clin Nutr, 1979 Apr, 32:4, 809-16
Abstract: The data presented confirm the advantages of Fe(III)-EDTA as a salt for iron fortification. This iron compound exchanges completely with intrinsic wheat iron in the lumen of the gut. The iron absorption data from this salt tested with six different food vehicles compared with the absorption of ferrous sulfate administered with the same vehicles indicate that while the mean absorption from ferrous sulfate varies from 2 to 30% according to the food vehicle mixed with the salt, the absorption from Fe(III)-EDTA remains practically the same. Apparently, the iron absorption from Fe(III)-EDTA complex is slightly or not affected by the presence of vegetable foods or milk. All these data suggest that only a small amount of iron from this salt, about 10 mg/day, would be necessary to prevent iron deficiency anemia even in those populations relying for their subsistence on vegetable food only.
Language of Publication: English
Unique Identifier: 79162501

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MeSH Heading (Major): Edetic Acid|*; Food, Fortified|*; Iron Chelates|*ME
MeSH Heading: Absorption; Anemia, Hypochromic|PC; Comparative Study; Dietary Carbohydrates; Female; Ferric Compounds|ME; Ferritin|BL; Ferrous Compounds|ME; Hemoglobins|ME; Human; Male; Structure-Activity Relationship; Sulfates; Transferrin|ME; Wheat

Publication Type: JOURNAL ARTICLE
ISSN: 0002-9165
Country of Publication: UNITED STATES

Record 12 from database: MEDLINE
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Title: Morphological and cytochemical study of Chlamydia with EDTA regressive technique and Gautier staining in ultrathin frozen sections of infected cell cultures: a comparison with embedded material.
Author: Popov V; Eb F; Lefebvre JF; Orfila J; Viron A
Address
Source: Ann Microbiol (Paris), 1978 Oct, 129 B:3, 313-37
Abstract: The cryo-ultramicrotomy technique was applied to study the ultrastructure of Chlamydia using two strains: one of C. psittaci and one of C. trachomatis. It clearly appeared that in both strains reticulate bodies show a high degree of plasticity, contrasting with the rigid spherical appearance of elementary bodies. Ultrastructural cytochemical study shows DNA fibrils dispersed throughout the cytoplasm in reticulate bodies whereas DNA is condensed in a nucleoid in elementary and intermediate bodies. The EDTA regressive technique reveals ribonucleoproteins in reticulate and elementary bodies of both studied strains.
Language of Publication: English
Unique Identifier: 79143304

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MeSH Heading (Major): Chlamydia psittaci|*UL; Chlamydia trachomatis|*UL; Edetic Acid|*; Frozen Sections|*; Microtomy|*
MeSH Heading: Animal; Bacteriological Techniques; Cattle; Cell Wall|AN/UL; Chick Embryo; Comparative Study; Culture Media; DNA, Bacterial|IP; Female; Histocytochemistry; Human; Microscopy, Electron; Ribonucleoproteins|IP

Publication Type: JOURNAL ARTICLE
ISSN: 0300-5410
Country of Publication: FRANCE

Record 13 from database: MEDLINE
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Title: [51Cr]EDTA plasma clearance and endogenous creatinine clearance in advanced renal insufficiency.
Author: Svendsen UG; Munck O; Czartoryski A; Stafanger G
Address
Source: Scand J Clin Lab Invest, 1978 Dec, 38:8, 777-9
Abstract: Comparison of [51Cr]EDTA plasma clearance corrected for extrarenal elimination with 24 h endogenous creatinine clearance in patients with advanced renal failure showed that the corrected [51Cr]EDTA clearance was lower than creatinine clearance, and thus might be a better approximation to the glomerular filtration rate in uraemic patients. The corrections cannot be used on [51cr]EDTA clearance values below the mean extrarenal clearance, averaging 3.7 ml/min.
Language of Publication: English
Unique Identifier: 79117847

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MeSH Heading (Major): Creatinine|*ME; Edetic Acid|*BL; Kidney Failure, Chronic|*ME
MeSH Heading: Adult; Aged; Chromium Radioisotopes; Comparative Study; Female; Human; Male; Middle Age

Publication Type: JOURNAL ARTICLE
ISSN: 0036-5513
Country of Publication: NORWAY

Record 14 from database: MEDLINE
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Title: Effects of Ca, Mg, and EDTA on creatine kinase activity in cerebrospinal fluid.
Author: Urdal P; Str‡mme JH
Address
Source: Clin Chem, 1979 Jan, 25:1, 147-50
Abstract: For one to obtain a precise estimate of creatine kinase (CK) activity in cerebrospinal fluid, the sample fraction is increased by about 10-fold over that used for serum. This increases the concentration of interfering substances, Ca being especially important. Therefore, the relationship between Ca, Mg, and EDTA was examined. Enzyme activity was maximal with 15 mmol of Mg per liter in the presence of 3 mmol of EDTA per liter, otherwise according to the (Scandinavian) recommended conditions for determination of CK activity in serum. These modifications increased the activity of CK by 35% for CK-MM and by 60% for CK-BB. Counteraction of Ca-induced inhibition was the main reason to this increase. We describe a practical and sensitive method for determining CK in cerebrospinal fluid.
Language of Publication: English
Unique Identifier: 79105833

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MeSH Heading (Major): Calcium|*PD; Creatine Kinase|*CF; Edetic Acid|*PD; Magnesium|*PD
MeSH Heading: Buffers; Enzyme Activation; Human; Kinetics

Publication Type: JOURNAL ARTICLE
ISSN: 0009-9147
Country of Publication: UNITED STATES

Record 15 from database: MEDLINE
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Title: Compensation of dietary induced reduction of tetracycline absorption by simultaneous administration of EDTA.
Author: Poiger H; Schlatter C
Address
Source: Eur J Clin Pharmacol, 1978 Nov 16, 14:2, 129-31
Abstract: The absorption of tetracycline in man under the influence of concomitantly administered EDTA, milk and a combination of EDTA and milk has been investigated. Urinary excretion of the drug was measured for 30 h. The inhibitory effect of milk could be counteracted by simultaneous ingestion of EDTA, which resulted in almost equivalent urinary excretion of tetracycline compared to experiments done in the fasting state. Administration of EDTA alone, in a neutral dosage form, did not significantly change absorption of the drug, which contradicted previous findings. The possible use of EDTA during tetracycline therapy is discussed.
Language of Publication: English
Unique Identifier: 79065107

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MeSH Heading (Major): Edetic Acid|*PD; Food|*; Intestinal Absorption|*DE; Tetracycline|*ME/UR
MeSH Heading: Adult; Female; Human; Male; Time Factors

Publication Type: JOURNAL ARTICLE
ISSN: 0031-6970
Country of Publication: GERMANY, WEST

Record 16 from database: MEDLINE
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Title: Isolation and characterization of outer and inner membranes from Pseudomonas aeruginosa and effect of EDTA on the membranes.
Author: Matsushita K; Adachi O; Shinagawa E; Ameyama M
Address
Source: J Biochem (Tokyo), 1978 Jan, 83:1, 171-81
Abstract: The outer and inner cytoplasmic membranes of Pseudomonas aeruginosa were separated as small and large membranes, respectively, from the cell envelope of this organism treated with lysozyme in Tris-chloride buffer containing sucrose and MgCl2 by differential centrifugation. The small membrane fraction contained predominantly 2-keto-3-deoxyoctonate (KDO), and little cytochromes or oxidase activities. The small membrane was composed of only 9 polypeptides and showed homogeneous small vesicles electron-microscopically. On the other hand, the large membrane fraction had high cytochrome contents and oxidase activities, and little KDO. The large membrane was composed of a number of polypeptides and showed large fragments or vesicles electron-microscopically. These results indicate that the small and large membranes are the outer and inner cytoplasmic membranes of P. aeruginosa, respectively. The isolated outer membrane showed a symmetrical protein peak with a density of 1.23 on sucrose density gradient centrifugation and the isolated inner membrane showed an unusually high density, probably due to association with ribosomes and extrinsic or loosely bound proteins. EDTA lowered the density of both membranes and caused lethal damage to the outer membrane, causing disintegration with the release of lipopolysaccharide (LPS), proteins and phospholipid.
Language of Publication: English
Unique Identifier: 78109407

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MeSH Heading (Major): Cell Membrane|*/AN/UL; Edetic Acid|*PD; Pseudomonas aeruginosa|*
MeSH Heading: Carbohydrate Dehydrogenases|AN; Cytochromes|AN; Female; Gluconates; Human; Ketoses|AN; Membrane Proteins|AN; NADH, NADPH Oxidoreductases|AN; Phospholipids|AN; Sugar Acids|AN

Publication Type: JOURNAL ARTICLE
ISSN: 0021-924X
Country of Publication: JAPAN

Record 17 from database: MEDLINE
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Title: Single injection (51Cr)EDTA plasma clearance determination in children using capillary blood samples.
Author: Brochner-Mortensen J; Christoffersen J
Address
Source: Scand J Clin Lab Invest, 1977 Nov, 37:7, 631-3
Abstract: The reliability of a determination of the total [51Cr]EDTA plasma clearance (E) (and with it the glomerular filtration rate), by a simplified single injection method (injected dose: 4.5 muCi per kg b.w.) using capillary blood samples (0.2 ml), was investigated in twenty children. Clearance values determined from capillary blood samples did not differ significantly from those measured simultaneously from venous blood samples, the mean ratio +/-SD being 1.02 +/- 0.06 (n = 10). The reproducibility (total day-to-day variation) of E determined from capillary blood samples was 6.7% in children with decreased renal function (n = 3) and 6.9% in children with normal renal function (n = 7). The present data indicate that the use of capillary blood samples is an accurate and very precise approach for determination of E in children.
Language of Publication: English
Unique Identifier: 78074717

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MeSH Heading (Major): Edetic Acid|*DU; Glomerular Filtration Rate|*
MeSH Heading: Child, Preschool; Chromium Radioisotopes|DU; Human; Infant; Methods

Publication Type: JOURNAL ARTICLE
ISSN: 0036-5513
Country of Publication: NORWAY

Record 18 from database: MEDLINE
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Title: Precision of single injection (51Cr)EDTA plasma clearance and endogenous creatinine clearance determinations in children.
Author: Brochner-Mortensen J; Rohbrandt K; Lauritzen RB
Address
Source: Scand J Clin Lab Invest, 1977 Nov, 37:7, 625-9
Abstract: The precision of two different clearance methods as used for routine assessment of the glomerular filtration rate (GFR) was investigated in thirty-one children aged 0.6-14 years: total [51Cr]EDTA plasma clearance (E) determined by a simplified single injection method; and 24 h endogenous creatinine clearance (C). Determination of C twice only succeeded in twenty children because of problems in collecting 24 h urine accurately. The precision (determined from the total day-to-day variation) for single determinations in patients with E greater than or equal to 80 ml/min/1.73 m2 was 5.5% for E and 13.8% for C. The corresponding figures for E less than 80 ml/min/1.73 m2 was 7.8% for E and 20.8% for C. Data in the literature on the inaccuracies of C and E versus GFR suggest that the degree of inaccuracy in predicting GFR from C is much higher than that from E, a feature which together with the present findings on precision indicates that E is much more reliable than C for routine determination of GFR in children.
Language of Publication: English
Unique Identifier: 78074716

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MeSH Heading (Major): Creatinine|*ME; Edetic Acid|*DU; Glomerular Filtration Rate|*
MeSH Heading: Adolescence; Child; Child, Preschool; Chromium Radioisotopes|DU; Comparative Study; Human; Infant; Methods

Publication Type: JOURNAL ARTICLE
ISSN: 0036-5513
Country of Publication: NORWAY

Record 19 from database: MEDLINE
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Title: IgG platelet antibodies in EDTA-dependent pseudothrombocytopenia bind to platelet membrane glycoprotein IIb.
Author: Fiorin F; Steffan A; Pradella P; Bizzaro N; Potenza R; De Angelis V
Address: Servizio Immunotrasfusionale, Ospedale Civile, San Donà di Piave, Italy.
Source: Am J Clin Pathol, 1998 Aug, 110:2, 178-83
Abstract: EDTA-dependent pseudothrombocytopenia (PTCP) consists of an inappropriate low platelet count caused by autoantibodies present in the serum samples reacting with platelets only in EDTA-anticoagulated blood. By using immunoprecipitation and Western blot techniques, we studied the immunochemical specificity of platelet agglutinating autoantibodies in the serum samples of 10 patients with PTCP. Furthermore, to evaluate a possible role of PTCP-associated IgG autoantibodies in increased platelet turnover, we assayed the plasma glycocalicin (GC) level and calculated the GC index for every patient. Our results provide direct evidence that an epitope located on platelet membrane glycoprotein IIb is recognized by PTCP-associated IgG antibodies; moreover GC levels in patients with EDTA-dependent PTCP were similar to control levels, thus excluding an increased platelet turnover. We conclude that antiplatelet antibodies directed against platelet cryptantigens are unlikely to have a major role in the increased removal of cells from circulation.
Language of Publication: English
Unique Identifier: 98368314

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MeSH Heading (Major): Autoantibodies|*IM; Blood Platelets|*IM; Edetic Acid|*; IgG|*IM; Platelet Glycoprotein GPIIb-IIIa Complex|*ME; Thrombocytopenia|*BL/*CI
MeSH Heading: Antibodies, Monoclonal|IM; Blotting, Western; Fluorescent Antibody Technique; Human; Platelet Count; Platelet Glycoprotein GPIb-IX Complex|AN; Precipitin Tests

Publication Type: JOURNAL ARTICLE
ISSN: 0002-9173
Country of Publication: UNITED STATES
CAS Registry/EC Number: 0 (glycocalicin); 0 (Antibodies, Monoclonal); 0 (Autoantibodies); 0 (IgG); 0 (Platelet Glycoprotein GPIb-IX Complex); 0 (Platelet Glycoprotein GPIIb-IIIa Complex); 60-00-4 (Edetic Acid)

Record 20 from database: MEDLINE
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Title: Metrizamide in urography. II. A comparison of 51Cr-EDTA clearance and metrizamide clearance in man.
Author: Golman K; Almen T; Denneberg T; Nosslin B
Address
Source: Invest Radiol, 1977 Jul-Aug, 12:4, 353-6
Abstract: In nine subjects undergoing urography with metrizamide measurements of total serum clearance of 51Cr-EDTA have been made before, during, and after the urography. During the urography both total serum clearances and renal clearances were determined for 51Cr-EDTA and metrizamide. The present study in man confirms the previous results from investigations in rabbits, that most of the intravenously injected metrizamide is excreted through the kidneys, that tubular reabsorption of metrizamide occurs and suggests that metrizamide might be used with advantage for urography.
Language of Publication: English
Unique Identifier: 77227155

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MeSH Heading (Major): Contrast Media|*ME; Edetic Acid|*ME; Iodobenzoates|*ME; Metrizamide|*ME; Urography|*
MeSH Heading: Chromium Radioisotopes; Comparative Study; Human

Publication Type: JOURNAL ARTICLE
ISSN: 0020-9996
Country of Publication: UNITED STATES

Record 21 from database: MEDLINE
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Title: Fe(III)-EDTA complex as iron fortification.
Author: Layrisse M; Martínez-Torres C
Address
Source: Am J Clin Nutr, 1977 Jul, 30:7, 1166-74
Abstract: Fe(III)-EDTA as iron fortification presents several advantages over the other iron salts previously used including ferrous sulfate. This iron compound exchange completely with vegetable food iron in the lumen of the gut but with the characteristics that the absorption from both, extrinsic and intrinsic food iron, is higher than that expected from other iron salfs. The comparison between the iron absorption from Fe(III)-EDTA and ferrous sulfate as iron fortification indicates that the absorption form EDTA is about twice as high than that observed from ferrous sulfate. The data indicates that only 10 to 15 mg of iron as Fe(III)-EDTA as iron fortification would be necessary to prevent iron deficiency anemia in population relying their subsistence of vegetable food only and free of parastic infection producing blood loss.
Language of Publication: English
Unique Identifier: 77219060

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MeSH Heading (Major): Edetic Acid|*AA/ME; Iron|*/ME
MeSH Heading: Absorption; Animal; Ascorbic Acid; Comparative Study; Corn; Female; Ferric Compounds; Ferrous Compounds; Food, Fortified; Hemoglobins|ME; Human; Male; Milk; Transferrin|ME

Publication Type: JOURNAL ARTICLE
ISSN: 0002-9165
Country of Publication: UNITED STATES

Record 22 from database: MEDLINE
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Title: Restoration of hemoglobin function in stored EDTA blood. Application in identification of hemoglobin variants with abnormal oxygen affinity.
Author: Sumoza A; Fairbanks VF; Pineda AA
Address
Source: Am J Clin Pathol, 1977 Jul, 68:1, 53-6
Abstract: The incubation of stored blood in a mixture of inosine, pyruvate, glucose, and phosphate restores the O2 affinity of hemoglobin to physiologic levels, as measured by the configuration of the dissociation curve and the P50. This regeneration of normal hemoglobin function not only is consistent for samples anticoagulated with EDTA and stored eight days at 4 C but also is demonstrable for at least 19 days of 4 C storage of EDTA-anticoagulated whole blood. This regeneration procedure is simple to perform and makes it possible to measure reliably O2 affinity in blood samples transmitted by mail.
Language of Publication: English
Unique Identifier: 77199151

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MeSH Heading (Major): Blood Preservation|*; Edetic Acid|*; Hemoglobins|*PH
MeSH Heading: Diphosphoglyceric Acids|BL; Female; Hemoglobins, Abnormal; Human; Male; Oxygen|BL; Oxygen Consumption

Publication Type: JOURNAL ARTICLE
ISSN: 0002-9173
Country of Publication: UNITED STATES

Record 23 from database: MEDLINE
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Title: GFR measurement with iohexol and 51Cr-EDTA. A comparison of the two favoured GFR markers in Europe.
Author: Brändström E; Grzegorczyk A; Jacobsson L; Friberg P; Lindahl A; Aurell M
Address: Department of Clinical Physiology, Sahlgrenska University Hospital, Göteborg, Sweden.
Source: Nephrol Dial Transplant, 1998 May, 13:5, 1176-82
Abstract: BACKGROUND: The aim of the present study was to compare the most commonly used GFR markers for clearance measurements, 51Cr-EDTA and iohexol, using two different methods for iohexol analysis, HPLC and X-ray fluorescence, referring both to the multiple-sample and single-sample calculations, using 51Cr-EDTA as the reference method. METHODS: Forty-nine patients with an estimated GFR >40 ml/min were included. 51Cr-EDTA and iohexol were injected simultaneously and blood samples were taken 150, 195 and 240 min after injection of the respective marker. RESULTS: The multiple-point clearances, determined from HPLC and X-ray fluorescence, compared to 51Cr-EDTA correlated highly (r=0.92 and 0.95 respectively). The results from single-point clearance comparison, iohexol measured by HPLC vs 51Cr-EDTA, yielded a correlation of r=0.91, while single-point clearance from iohexol, analysed by X-ray fluorescence, obtained a correlation of 0.93 and an intercept statistically different from origo. CONCLUSIONS: Iohexol and 51Cr-EDTA are comparable as GFR markers for multiple-point clearance measurements. The single-sample method for GFR >40 ml/min can be used with a high accuracy. The precision and accuracy of X-ray fluorescence analysis of low concentrations of iohexol were less than those of HPLC. Care should therefore be taken when using X-ray fluorescence that the injected dose of iohexol should result in a plasma concentration level of iodine of at least 0.06 mg/ml at the time of blood sampling.
Language of Publication: English
Unique Identifier: 98285008

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MeSH Heading (Major): Edetic Acid|*DU; Glomerular Filtration Rate|*/PH; Iohexol|*DU
MeSH Heading: Adult; Aged; Aged, 80 and over; Biological Markers|AN; Chromatography, High Pressure Liquid; Chromium Radioisotopes|DU; Comparative Study; Europe; Evaluation Studies; Female; Human; Male; Middle Age; Spectrometry, X-Ray Emission

Publication Type: JOURNAL ARTICLE
ISSN: 0931-0509
Country of Publication: ENGLAND
CAS Registry/EC Number: 0 (Biological Markers); 0 (Chromium Radioisotopes); 60-00-4 (Edetic Acid); 66108-95-0 (Iohexol)

Record 24 from database: MEDLINE
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Title: Tissue inhibitor of metalloproteinase-2 protection of matrix metalloproteinase-2 from degradation by plasmin is reversed by divalent cation chelator EDTA and the bisphosphonate alendronate.
Author: Farina AR; Tacconelli A; Teti A; Gulino A; Mackay AR
Address: Department of Experimental Medicine, University of L'Aquila, Italy. farina@aquila.infn.it
Source: Cancer Res, 1998 Jul 15, 58:14, 2957-60
Abstract: The degradation of tissue inhibitor of metalloproteinase (TIMP)-free matrix metalloproteinase (MMP)-2 to proteolytically inactive fragments by plasmin was inhibited in equimolar mixtures of purified TIMP-2 and TIMP-free MMP-2 and was not observed in purified MMP-2-TIMP-2 complexes. Divalent cation chelators EDTA and sodium Alendronate did not inhibit plasmin degradation of TIMP-free MMP-2 but reversed the ability of TIMP-2 to protect MMP-2 from degradation by plasmin. Our data confirm a role for plasmin in the clearance of TIMP-free MMP-2, identify a pivotal role for TIMP-2 in regulating MMP-2 longevity in plasmin-containing environments, and highlight a novel therapeutic use for chelators of divalent cations, including the bisphosphonate Alendronate, in the reversal of TIMP-2 protection of MMP-2 from degradation by plasmin. We propose that these observations are relevant to pathologies that are dependent upon plasmin and MMP-2 activity (e.g., tumor invasion and metastasis).
Language of Publication: English
Unique Identifier: 98343547

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MeSH Heading (Major): Gelatinases|*DE/ME; Metalloproteinases|*DE/ME; Plasmin|*AI/ME; Protease Inhibitors|*PD; Tissue Inhibitor-of Metalloproteinase-2|*PD
MeSH Heading: Alendronate|PD; Cations; Chelating Agents|PD; Diphosphonates|PD; Edetic Acid|PD; Human; Support, Non-U.S. Gov't; Tumor Cells, Cultured|DE

Publication Type: JOURNAL ARTICLE
ISSN: 0008-5472
Country of Publication: UNITED STATES
CAS Registry/EC Number: EC 3.4.21.7 (Plasmin); EC 3.4.24 (Metalloproteinases); EC 3.4.24.24 (gelatinase A); EC 3.4.99.- (Gelatinases); 0 (Cations); 0 (Chelating Agents); 0 (Diphosphonates); 0 (Protease Inhibitors); 127497-59-0 (Tissue Inhibitor-of Metalloproteinase-2); 60-00-4 (Edetic Acid); 66376-36-1 (Alendronate)

Record 25 from database: MEDLINE
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Title: Effects of smoking on the urine excretion of oral 51Cr EDTA in ulcerative colitis.
Author: Benoni C; Prytz H
Address: Department of Medicine, University Hospital of MalmÂo, Sweden.
Source: Gut, 1998 May, 42:5, 656-8
Abstract: BACKGROUND: Smokers have a reduced risk and ex-smokers an increased risk of ulcerative colitis (UC). Stopping smoking often precedes onset and relapses. Smoking reduces the 24 hour urine excretion of oral chromium-51 labelled EDTA in healthy individuals. AIMS: To estimate the effects of smoking on the urine excretion of oral 51Cr EDTA in well characterised patients with UC. SUBJECTS: Sixteen smoking and 16 non-smoking patients with UC in remission were studied. The non-smokers had never smoked. Most were taking 5-aminosalicylic acid. No patient took steroids or immunosuppressants. The control group comprised 25 smoking healthy volunteers and 25 who had never smoked. The median cigarette consumption was equal in the patients and volunteers. METHODS: The 24 hour urine excretion of oral 51Cr EDTA was measured and the results were correlated with smoking habits, number of cigarettes, and disease extent. RESULTS: Patients with UC had significantly higher 24 hour urine recoveries than healthy controls (p = 0.04). This difference was more pronounced when patients who smoked were compared with healthy smokers (p = 0.005) No significant differences were found when comparing non-smoking patients with non-smoking controls or when comparing smoking and non-smoking patients. Urine recoveries did not correlate with number of cigarettes or disease extent. Smoking was more prevalent in patients with a more limited disease extent (p = 0.033). CONCLUSIONS: Effects of smoking on the urine excretion of 51Cr EDTA in health were abolished by the presence of UC. The protective effects of smoking in established UC are not due to a moderating effect of smoking on intestinal permeability.
Language of Publication: English
Unique Identifier: 98323295

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MeSH Heading (Major): Colitis, Ulcerative|*UR; Edetic Acid|*ME; Smoking|*UR
MeSH Heading: Adult; Aged; Chromium Radioisotopes|ME; Female; Human; Intestinal Absorption; Male; Middle Age; Support, Non-U.S. Gov't

Publication Type: JOURNAL ARTICLE
ISSN: 0017-5749
Country of Publication: ENGLAND

Record 26 from database: MEDLINE
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Title: Ultrasonic subgingival root planing and EDTA etching in a one-step procedure.
Author: Blomlöf J; Blomlöf L; Lindskog S
Address: Department of Basic Oral Sciences, School of Dentistry, Karolinska Institutet, Huddinge, Stockholm, Sweden.
Source: Swed Dent J, 1997, 21:6, 213-9
Abstract: The purpose of the present study was to investigate if ultrasonic debridement and EDTA etching could be combined in a ore-step procedure. Tap water was exchanged for a 24% EDTA solution as irrigation agent during root planing with a piezo scaler. 24 human teeth extracted due to severe periodontal disease were used and the results were evaluated with scanning electron microscopy. Results indicate that synergistic effects (smear removal and exposure of collagen fibers) were negligible with conventional application of the irrigation fluid to the working area of the scaling tip while a marked effect was evident when the EDTA solution was applied directly to the working area of the piezoelectric scaler through a customized tip.
Language of Publication: English
Unique Identifier: 98166232

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MeSH Heading (Major): Acid Etching, Dental|*MT; Chelating Agents|AD/*TU; Edetic Acid|AD/*TU; Root Planing|*/IS/MT; Subgingival Curettage|*/IS/MT; Ultrasonic Therapy|*/IS/MT
MeSH Heading: Collagen|UL; Dental Cementum|UL; Dental Scaling|IS/MT; Dentin|UL; Human; Irrigation; Microscopy, Electron, Scanning; Periodontitis|TH; Water

Publication Type: CLINICAL TRIAL; JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
ISSN: 0347-9994
Country of Publication: SWEDEN

Record 27 from database: MEDLINE
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Title: Lead exposure and accumulation in healthy Thais: assessed by lead levels, EDTA mobilization and heme synthesis-related parameters.
Author: Wananukul W; Sirivarasai J; Sriapha C; Chanatara V; Chunvimaluang N; Keanpoompuang A; Boriboon W; Pumala K; Kaojarern S
Address: Department of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok Thailand.
Source: J Med Assoc Thai, 1998 Feb, 81:2, 110-6
Abstract: Lead is one of the pollutants which is of public concern. The magnitude of lead contamination in Thai people is of interest. The objective of this study was to evaluate the lead status in normal healthy volunteers. Normal volunteers were included. The blood for lead level, Zinc protoporphyrin (ZPP), delta-aminolevulinic acid dehydratase (ALA-D) activity, and baseline urine for lead, delta-aminolevulinic acid (ALA) and coproporphyrinogen III (CP3) were collected. The EDTA mobilization test was done. 24 hour urine after administration of the drug was collected for lead analysis. Thirty volunteers were included in the study. All were men whose average age was 32.5 +/- 6.9 years. The mean lead level was 5.95 +/- 2.01 micrograms/dL and 5.83 +/- 2.32 micrograms/L in urine. The 24 hour urine lead contents before and after EDTA administration were significantly different (11.11 +/- 6.72 and 16.05 +/- 9.51 micrograms respectively). Blood ALA-D activity was 251.6 +/- 80.4 unit/ml of RBC. Urine ALA and CP3 were 0.56 +/- 1.2 mg/L and 22.17 +/- 23.9 micrograms/L respectively. All were in the normal ranges. All parameters suggested that the healthy Thai volunteers had an acceptable magnitude of lead exposure and accumulation.
Language of Publication: English
Unique Identifier: 98190745

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MeSH Heading (Major): Edetic Acid|DU/*ME; Environmental Exposure|AE/*AN; Environmental Monitoring|*; Heme|*BI/ME; Lead|AN/*BL/*UR
MeSH Heading: Adult; Creatinine|AN/UR; Human; Male; Middle Age; Reference Values; Support, Non-U.S. Gov't; Thailand; Urinalysis

Publication Type: CLINICAL TRIAL; JOURNAL ARTICLE
ISSN: 0125-2208
Country of Publication: THAILAND

Record 28 from database: MEDLINE
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Title: Defining the orientation of the human U1A RBD1 on its UTR by tethered-EDTA(Fe) cleavage.
Author: Beck DL; Stump WT; Hall KB
Address: Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Source: RNA, 1998 Mar, 4:3, 331-9
Abstract: The N-terminal RNA binding domain of the human U1A protein (RBD1) specifically binds an RNA hairpin of U1 snRNA as well as two internal loops in the 3' UTR of its own mRNA. Here, a single cysteine has been introduced into Loop 1 of RBD1, which is subsequently used to attach (EDTA-2-aminoethyl) 2-pyridyl disulfide-Fe3+ (EPD-Fe). This EDTA-Fe derivative is used to generate hydroxyl radicals to cleave the proximal RNA sugar-phosphate backbone in the RNA-RBD complexes. RBD1(K20C)-EPD-Fe cleaves the 5' strand of the RNA hairpin stem, centered four base pairs away from the base of the loop, and cleaves the UTR in two places, again centered on the 5' side of the fourth base pair from each internal loop. These data, extrapolated to the position of Lys 20 in RBD1, orient the two proteins bound to the UTR, and provide direct biochemical evidence for the proposed model of the RBD1:UTR complex.
Language of Publication: English
Unique Identifier: 98169154

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MeSH Heading (Major): Ribonucleoproteins, Small, U1|CH/*GE/*ME; RNA|CH/*ME; Translation, Genetic|*
MeSH Heading: Base Sequence; Binding Sites; Edetic Acid|AA/CH/ME; Human; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Nucleic Acid Conformation; Protein Conformation; RNA-Binding Proteins|CH/GE/ME; RNA, Small Nuclear|ME; Support, Non-U.S. Gov't; Support, U.S. Gov't, P.H.S.

Publication Type: JOURNAL ARTICLE
ISSN: 1355-8382
Country of Publication: UNITED STATES

Record 29 from database: MEDLINE
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Title: Essential monoclonal gammopathy with an IgM paraprotein that is a cryoglobulin with cold agglutinin and EDTA-dependent platelet antibody properties.
Author: Pujol M; Ribera JM; Jimenez C; Ribera A; Abad E; Feliu E
Address: Servicio de HematologÆia y Hemoterapia del Hospital Universitario Germans Trias y Pujol, Badalona, Spain.
Source: Br J Haematol, 1998 Mar, 100:3, 603-4
Abstract: A patient with apparent anaemia and thrombocytopenia caused by a monoclonal paraprotein is described. The patient's serum contained a monoclonal IgM kappa, a cryoglobulin and a cold agglutinin. The cryoglobulin, similar to the serum paraprotein, was a monoclonal IgM kappa. Serum was studied to determine the relationship of the cryoglobulin with the cold agglutinin. The cryoglobulin and cold agglutinin were found to be the same paraprotein. Moreover, with absorption and elution techniques the reactivity of the autoantibody with both erythrocytes and platelets was demonstrated. Reports of cryoprecipitable cold agglutinins are rare and therefore this case is exceptional given that the IgM kappa paraprotein was found to be a cold agglutinin which was also reactive with platelets.
Language of Publication: English
Unique Identifier: 98163178

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MeSH Heading (Major): Cryoglobulins|*IM; IgM|*BL; Paraproteinemias|*IM
MeSH Heading: Aged; Anemia, Hemolytic, Autoimmune|IM; Autoantibodies|IM; Blood Platelets|IM; Case Report; Edetic Acid; Erythrocytes|IM; Female; Human; Thrombocytopenia|IM

Publication Type: JOURNAL ARTICLE
ISSN: 0007-1048
Country of Publication: ENGLAND

Record 30 from database: MEDLINE
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Title: The role of EDTA in provoking allergic reactions to subcutaneous infusion of apomorphine in patients with Parkinson's di